Determination of urinary norandrosterone excretion in females during one menstrual cycle by gas chromatography/mass spectrometry

Biomedical Chromatography

Volumn 20, Issue 8, 2006, Pages 710-717

  Hemmersbach, Peter ^a   Hagensen Jetne A H ^a   Lund, H S ^a  

^a AKER UNIVERSITY HOSPITAL   (Norway)

Author keywords

Aromatization; Doping control; Nandrolone; Norandrosterone

Indexed keywords

BODY FLUIDS; PHYSIOLOGY;

ANABOLIC STEROID; DOPING CONTROL; FOLLICULAR FLUID; GASCHROMATOGRAPHY-MASS SPECTROMETRY; MENSTRUAL CYCLE; NANDROLONE; NORANDROSTERONE; PEAK CONCENTRATIONS; URINARY EXCRETION; URINARY METABOLITES;

GAS CHROMATOGRAPHY;

ANDROSTERONE DERIVATIVE; LUTEINIZING HORMONE; NORANDROSTERONE; UNCLASSIFIED DRUG;

AROMATIZATION; ARTICLE; CONTROLLED STUDY; DOPING; FEMALE; FOLLICULAR PHASE; GAS CHROMATOGRAPHY; HORMONE DETERMINATION; HORMONE SYNTHESIS; HORMONE URINE LEVEL; HUMAN; HUMAN EXPERIMENT; LUTEAL PHASE; MASS SPECTROMETRY; MENSTRUAL CYCLE; NORMAL HUMAN; QUANTITATIVE ANALYSIS; SCHOOL CHILD; URINALYSIS; URINARY EXCRETION; VOLUNTEER;

EID: 33745826456     PISSN: 02693879     EISSN: 10990801     Source Type: Journal    
DOI: 10.1002/bmc.586     Document Type: Article

References (39)

1. Ayotte C, Levesque JF, Cleroux M, Lajeunesse A, Goudreault D and Fakirian A. Sport nutritional supplements: quality and doping controls. Canadian Journal of Applied Physiology 2001; 26(suppl.): S120.
2. Bagchus WM, Smeets JM, Verheul HA, De Jager-Van Der Veen SM, Port A and Geurts TB. Pharmacokinetic evaluation of three different intramuscular doses of nandrolone decanoate: analysis of serum and urine samples in healthy men. Journal of Clinical Endocrinology and Metabolism 2005; 90: 2624.
3. 13C]Nandrolone excretion in trained athletes: interindividual variability in metabolism. Clinical Chemistry 2004; 50: 355.
4. Callicott R, Kicman AT and Expert Committee on Nandrolone UKSC. Nandrolone Progress Report to the UK Sports Council from the Expert Committee on Nandrolone February 2003. International Journal of Sports Medicine 2003; 24: 620.
5. Catlin DH, Kammerer RC, Hatton CK, Sekera MH and Merdink JL. Analytical Chemistry at the Games of the XXIIIrd Olympiad in Los Angeles, 1984. Clinical Chemistry 1987; 33: 319
6. Catlin DH, Leder BZ, Ahrens B, Starcevic B, Hatton CK, Green GA and Finkelstein JS. Trace contamination of over-the-counter androstenedione and positive urine test results for a nandrolone metabolite. JAMA 2000; 284: 2618.
7. de Boer D, de Jong E, Maes RA and van Rossum JM. The problems of oral contraceptives in dope control of anabolic steroids. Biomedical and Environmental Mass Spectrometry 1988; 17: 127.
8. de Boer D, Bensink SN, Borggreve AR, van Ooijen RD and Maes RA. Profiling 19-norsteroids. III. GC/MS/MS analysis of 19-norsteroids during pregnancy. In 10th Cologne Workshop on Dope Analysis, Donike M, Geyer H, Gotzmann A, Mareck-Engelke U and Rauth S (eds). Sport und Buch Strauß: Köln, 1993: 121.
9. Debruyckere G, Van Peteghem CH and de Sagher R. Influence of the consumption of meat contaminated with anabolic steroids on doping tests. Analytica Chimica Acta 1993; 275: 49
10. de Geus B, Delbeke F, Meeusen R, Van Eenoo P, De Meirleir K and Busschaert B. Norandrosterone and noretiocholanolone concentration before and after submaximal standardized exercise. International Journal of Sports Medicine 2004; 25: 528.
11. Dehennin L, Silberzahn P, Reiffsteck A and Zwain I. Presence de 19-norandrostendione et de 19-nortestosterone dans les fluides folliculaires humain et equin. Pathological Biology 1984; 32: 828.
12. Dehennin L, Jondet M and Schöller R. Androgen and 19-norsteroid profiles in human preovulatory follicles from stimulated cycles: an isotope dilution-mass spectrometric study. Journal of Steroid Biochemistry 1987; 26: 399.
13. Dehennin L, Bonnaire Y and Plou P. Urinary excretion of 19-norandrosterone of endogenous origin in man: quantitative analysis by gas chromatography-mass spectrometry. Journal of Chromatography B 1999; 721: 301.
14. De Wasch K, Le Bizec B, De Brabander H, Andre F and Impens S. Consequence of boar edible tissue consumption on urinary profiles of nandrolone metabolites. II. Identification and quantification of 19-norsteroids responsible for 19-norandrosterone and 19-noretiocholanolone excretion in human urine. Rapid Communications in Mass Spectrometry 2001; 15: 1442.
15. Donike M, Zimmermann J, Bärwald KR, Schänzer W, Christ V, Klostermann K and Opfermann G. Routinebestimmung von Anabolika im Harn. Deutsche Zeitschrift für Sportmedizin 1984; 14.
16. Donike M, Geyer H, Gotzmann A, Kraft M, Mandel F, Nolteernsting E, Opfermann G, Sgmund G and Schänzer W. Dope analysis. In International Athletic Foundation: First World Symposium on Doping in Sport, Belotti P, Benzi G and Ljungqvist A (eds). International Athletic Foundation: Florence, 1987; 53.
17. Engel LL, Alexander L and Wheeler M. Urinary metabolites of administered 19-nortestosterone. Journal of Biological Chemistry 1958; 231: 159.
18. Galan Martin AM, Marino JI, Garcia de Tiedra MP, Marabe JJ, Caballero Loscos MJ and Marino MM. Determination of nandrolone and metabolites in urine samples from sedentary persons and sportsmen. Journal of Chromatography B: Biomedical Science Applications 2001; 761: 229.
19. Gambelunghe C, Sommavilla M and Rossi R. Testing for nandrolone metabolites in urine samples of professional athletes and sedentary subjects by GC/MS/MS analysis. Biomedical Chromatography 2002; 16: 508.
20. Geyer H, Mareck-Engelke U, Reinhart U, Thevis M and Schänzer W. Positive doping cases with norandrosterone after application of contaminated nutritional supplements. Deutsche Zeitschrift für Sportmedizin 2000; 51: 378.
21. Grosse J, Anielski P, Hemmersbach P, Lund H, Müller KR, Rautenberg C and Thieme D. Formation of 19-norsteroids by in situ demethylation of endogenous steroids in stored urine samples. Steroids 2005; 70: 499.
22. Hemmersbach P, Bjerke B, Birkeland KI and Haug E. Short report from the doping analysis during the XVII Olympic Winter Games. In Advances in Doping Analysis - Proceedings from the 12th Cologne Workshop on Doping Analysis, Donike M, Geyer H, Gotzmann A and Mareck-Engelke U (eds). Sport und Buch Strauß: Köln, 1995; 393.
23. Jeanneau T, Kintz P, Cirimele V and Ludes B. Détermination des concentrations physiologiques de la norandrosterone et de la noretiocholanolone, métabolites urinaires de la nandrolone par CPG/ SM. Toxicorama 1999; 11: 25.
24. Kintz P, Cirimele V and Ludes B. Norandrosterone et noretiocholanolone: les métabolites révélateurs. Acta Clinica Belgica Supplements 1999; 68.
25. Kupfer D, Forchielli E and Dorfmann RI. 3a-Hydroxy-19-nor-5a-androstan-17-on and 19-nor-5a-androstane-3a.17b-diol. Journal of Organic Chemistry 1960; 25: 1674.
26. Le Bizec B, Monteau F, Gaudin I and André F. Evidence for the presence of endogenous 19-norandrosterone in human urine. Journal of Chromatography B 1999; 723: 157.
27. Le Bizec B, Gaudin I, Monteau F, Andre F, Impens S, De Wasch K and De Brabander H. Consequence of boar edible tissue consumption on urinary profiles of nandrolone metabolites. I. Mass spectrometric detection and quantification of 19-norandrosterone and 19-noretiocholanolone in human urine. Rapid Communications in Mass Spectrometry 2000; 14: 1058.
28. Le Bizec B, Bryand F, Gaudin I, Monteau F, Poulain F and Andre F. Endogenous nandrolone metabolites in human urine. Two-year monitoring of male professional soccer players. Journal of Analytical Toxicology 2002a; 26: 43.
29. Le Bizec B, Bryand F, Gaudin I, Monteau F, Poulain F and Andre F. Endogenous nandrolone metabolites in human urine: preliminary results to discriminate between endogenous and exogenous origin. Steroids 2002b; 67: 105.
30. Lund HS, Jåthun S, Fedorcsak P, Storeng R, Torjesen P and Hemmersbach P. Synthesis of nandrolone in the human ovary. In Recent Advances in Doping Analysis (10), Schänzer W, Geyer H, Gotzmann A, and Mareck U (eds). Sport & Buch Strauß: Köln, 2002; 23.
31. Mareck-Engelke U, Schultze G, Geyer H and Schänzer W. 19-Norandrosterone in pregnant women. In Recent Advances in Doping Analysis (8), Schänzer W, Geyer H, Gotzmann A, and Mareck-Engelke U (eds). Sport & Buch Strauß: Köln, 2000; 145.
32. Reznik Y, Herrou M, Dehenin L, Lamaire M and Leymarie P. Rising plasma levels of 19-nortestosterone throughout pregnancy: determination by radioimmunoassay and validation by gas chromatography-mass spectrometry. Journal of Clinical Endocrinology Metabolism 1987; 64: 1086.
33. Reznik Y, Dehennin L, Coffin C, Mahoudeau J and Leymarie P. Urinary nandrolone metabolites of endogenous origin in man: A confirmation by output regulation under human chorionic gonadotropin stimulation. Journal of Clinical Endocrinology and Metabolism 2001; 86: 146.
34. Robinson N, Taroni F, Saugy M, Ayotte C, Mangin P and Dvorak J. Detection of nandrolone metabolites in urine after a football game in professional and amateur players: a Bayesian comparison. Forensic Science International 2001; 122: 130.
35. Schänzer W. Metabolism of anabolic androgenic steroids. Clinical Chemistry 1996; 42: 1001.
36. Schänzer W, Horning S, Opfermann G and Donike M. Gas chromatography/ mass spectrometry identification of long-term excreted metabolites of the anabolic steroid 4-chloro-1,2-dehydro-17-alpha-methyltestosterone in humans. Journal of Steroid Biochemistry and Molecular Biology 1996; 57: 363.
37. UK Sport. Nandrolone Review - Report to the UK Sports Council from the Expert Committee. UK Sports Council: London, 2000; 20.
38. Van Eenoo P, Delbeke FT, de Jong FH and De Backer P. Endogenous origin of norandrosterone in female urine: indirect evidence for the production of 19-norsteroids as by-products in the conversion from androgen to estrogen. Journal of Steroid Biochemistry and Molecular Biology 2001; 78: 351.
39. World Anti-Doping Agency. Technical Document TD 2003TDCR, 2003. Available at http://www.wada-ama.org/rtecontent/document/criteria_1_2.pdf (access date 3 October 2005).