Introduction of HPLC/orbitrap mass spectrometry as screening method for doping control

Journal of Mass Spectrometry

Volumn 43, Issue 7, 2008, Pages 949-957

  Virus, E D ^a   Sobolevsky, T G ^a   Rodchenkov, G M ^a  

^a Federal Antidoping Center   (Russian Federation)

Author keywords

2 agonists; Doping control; Exogenous anabolic steroids; High resolution mass spectrometry; Orbitrap

Indexed keywords

ARSENIC COMPOUNDS; ATMOSPHERIC PRESSURE; ATMOSPHERICS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY;

ATMOSPHERIC PRESSURE CHEMICAL IONIZATION (APCI); COLLISION INDUCED DISSOCIATION (CID); DOPING AGENTS; DOPING CONTROL; HUMAN URINE; SCREENING METHODS;

ATMOSPHERIC IONIZATION;

ANABOLIC AGENT; ANDROSTERONE DERIVATIVE; ANTIESTROGEN; BETA 2 ADRENERGIC RECEPTOR STIMULATING AGENT; BOLASTERONE; BOLDENONE; CALUSTERONE; CLENBUTEROL; CLOSTEBOL ACETATE; EFAPROXIRAL; FLUOXYMESTERONE; FORMESTANE; FURAZABOL; GESTRINONE; MESTEROLONE; METANDIENONE; METENOLONE; METHYLTESTOSTERONE; METRIBOLONE; NANDROLONE; OXANDROLONE; OXYMESTERONE; SALBUTAMOL; SALMETEROL; STANOZOLOL; TETRAHYDROGESTRINONE; TRENBOLONE; UNINDEXED DRUG; ZEARALENONE; ZERANOL;

ACCURACY; ARTICLE; DOPING; DRUG DETERMINATION; DRUG SCREENING; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; MASS SPECTROMETRY; PRIORITY JOURNAL; REPRODUCIBILITY; URINALYSIS;

ADRENERGIC BETA-AGONISTS; ANABOLIC AGENTS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; DOPING IN SPORTS; ESTROGEN RECEPTOR MODULATORS; HUMANS; MASS SCREENING; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; SUBSTANCE ABUSE DETECTION;

EID: 47749089748     PISSN: 10765174     EISSN: 10969888     Source Type: Journal    
DOI: 10.1002/jms.1447     Document Type: Article

References (18)

1. Ayotte C, Goudreault D, Charlebois A. Testing for natural and synthetic anabolic agents in human urine. Journal of Chromatography 1996; 687: 3, DOI:10.1016/S0378-4347(96)000 32-1.
2. Schänzer W, Delahaut P, Geyer H, Machnik M, Horning S. Long-term detection and identification of metandienone and stanozolol abuse in athletes by gas chromatography-high-resolution mass spectrometry. Journal of Chromatography. B, Biomedical Sciences and Applications 1996; 687: 93, DOI:10.1016/S0378-4347(96)00187-9.
3. Marcos J, Pascual JA, de la Torre X, Segura J. Fast screening of anabolic steroids and other banned doping substances in human urine by gas chromatography/tandem mass spectrometry. Journal of Mass Spectrometry 2002; 37: 1059, DOI:10.1002/jms.365.
4. Leinonen A, Kuuranne T, Kotiaho T, Kostiainen R. Screening of free 17-alkyl-substituted anabolic steroids in human urine by liquid chromatography-electrospray ionization tandem mass spectrometry. Steroids 2004; 69: 101, DOI:10.1016/j.steroids.2003.10.007.
5. Deventer K, Eenoo P, Delbeke FT. Screening for anabolic steroids in doping analysis by liquid chromatography/electrospray ion trap mass spectrometry. Biomedical Chromatography 2006; 20: 429, DOI: 10.1002/bmc.579.
6. Pozo OJ, Van Eenoo P, Deventer K, Delbeke FT. Development and validation of a qualitative screening method for the detection of exogenous anabolic steroids in urine by liquid chromatography-tandem mass spectrometry. Analytical and Bioanalytical Chemistry 2007; 389: 1209, DOI: 10.1007/s00216-007-1530-6.
7. Thevis M, Geyer H, Rodchenkov G, Maraceck U, Schanzer W. Detection of stanozolol and its major metabolites in human urine by liquid chromatography-tandem mass spectrometry. Chromatographic 2006; 64: 441, DOI: 10.1365/s10337-006-0043-3.
8. Thevis M, Schanzer W. Current role of LC-MS(/MS) in doping control. Journal of Analytical and Bioanalytical Chemistry 2007; 388: 1351, DOI: 10.1007/s00216-007-1131-4.
9. Georgakopoulos CG, Vonaparti A, Stamou M, Kiousi P, Lyris E, Angelis YS, Tsoupras G, Wuest B, Nielen MW, Panderi I, Koupparis M. Preventive doping control analysis: liquid and gas chromatography time-of-flight mass spectrometry for detection of designer steroids. Rapid Communications in Mass Spectrometry 2007; 21: 2439, DOI: 10.1002/rcm.3103.
10. Touber ME, van Engelen MC, Georgakopoulos C, van Rhijn JA, Nielen MWF. Multi-detection of corticosteroids in sports doping and veterinary control using high-resolution liquid chromatography/time-of-flight mass spectrometry. Analytica Chimica Acta 2007; 586: 137, DOI:10.1016/j.aca.2006.09. 058.
11. Kolmonen M, Leinonen A, Pelander A, Ojanperä I. A general screening method for doping agents in human urine by solid phase extraction and liquid chromatography/time-of-flight mass spectrometry. Analytica Chimica Acta 2007; 585: 94, DOI: 10.1016/j.aca.2006.12.028.
12. Thevis M, Makarov AA, Horning S, Schänzer W. Mass spectrometry of stanozolol and its analogues using electrospray ionization and collision-induced dissociation with quadrupolelinear ion trap and linear ion trap-orbitrap hybrid mass analyzers. Rapid Communications in Mass Spectrometry 2005; 19: 3369, DOI: 10.1002/rcm.2204.
13. Schänzer W, Geyer H, Fußhöller G, Halatcheva N, Kohler M, Parr M, Guddat S, Thomas A, Thevis M. Mass spectrometric identification and characterization of a new long-term metabolite of metandienone in human urine. Rapid Communications in Mass Spectrometry 2006; 20: 2252, DOI: 10.1002/rcm.2587.
14. Makarov A. Electrostatic axially harmonic orbital trapping: a high-performance technique of mass analysis. Analytical Chemistry 2000; 72: 1156, DOI: 10.1021/ac991131p.
15. Eurachem Working Group. A Laboratory Guide to Method Validation and Related Topics. Eurachem: Teddington, 1998.
16. Thevis M, Geyer H, Mareck U, Schänzer W. Screening for unknown synthetic steroids in human urine by liquid chromatography-tandem mass spectrometry. Journal of Mass Spectrometry 2005; 40: 955, DOI: 10.1002/jms.873.
17. Thevis M, Bommerich U, Opfermann G, Schänzer W. Characterization of chemically modified steroids for doping control purposes by electrospray ionization tandem mass spectrometry. Journal of Mass Spectrometry 2005; 40: 490, DOI: 10.1002/jms.820.
18. Guan F, Soma LR, Luo Y, Uboh CE, Peterman S. Collision-induced dissociation pathways of anabolic steroids by electrospray ionization tandem mass spectrometry. Journal of the American Society for Mass Spectrometry 2006; 17: 477, DOI:10.1016/j.jasms.2005.11.021.