Neurotoxic Effect of the Complex of the Ovine Prion Protein (OvPrP C) and RNA on the Cultured Rat Cortical Neurons

Neurochemical Research

Volumn 36, Issue 10, 2011, Pages 1863-1869

  Liu, Mei Li ^a   Wen, Jian Jun ^c   Xu, Xue Fang ^b   Zhao, De Ming ^b  

^a BEIHANG UNIVERSITY   (China)

^b CHINA AGRICULTURAL UNIVERSITY   (China)

^c Ganzhou People's Hospital   (China)

Author keywords

Bax; Neuronal injury neurotoxicity; OvPrP C RNA; Prion

Indexed keywords

PROTEIN BAX; RECOMBINANT PRION PROTEIN RNA NUCLEOPROTEIN COMPLEX; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; BETA SHEET; BRAIN CELL; CELL DEATH; CONTROLLED STUDY; CYTOTOXICITY TEST; IMMUNOPRECIPITATION; NERVE CELL DEGENERATION; NEUROTOXICITY; NONHUMAN; PRION DISEASE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN RNA BINDING; RAT; WESTERN BLOTTING;

ANIMALS; BCL-2-ASSOCIATED X PROTEIN; CELL DEATH; CELLS, CULTURED; CEREBRAL CORTEX; HUMANS; IN SITU NICK-END LABELING; NEURONS; NEUROTOXICITY SYNDROMES; PRION DISEASES; PRPC PROTEINS; RATS; RECOMBINANT PROTEINS; RNA; SHEEP;

OVIS; RATTUS;

EID: 80555126822     PISSN: 03643190     EISSN: 15736903     Source Type: Journal    
DOI: 10.1007/s11064-011-0506-2     Document Type: Article

References (34)

1. Prusiner SB (1998) Prions. Proc Natl Acad Sci USA 95:13363-13383 (Pubitemid 28525623)
2. Xiong LW, Raymond LD, Hayes SF, Raymond GJ, Caughey B (2001) Conformational change aggregation and fibril formation induced by detergent treatments of cellular prion protein. J Neurochem 79:669-678 (Pubitemid 33029940)
3. Adler V, Zeiler B, Kryukov V, Kascsak R, Rubenstein R, Grossman A (2003) Small, highly structured RNAs participate in the conversion of human recombinant PrP(Sen) to PrP(Res) in vitro. J Mol Biol 332:47-57 (Pubitemid 36999730)
4. Deleault NR, Lucassen RW, Supattapone S (2003) RNA molecules stimulate prion protein conversion. Nature 425:717-720 (Pubitemid 37314314)
5. Yang S, Thackray AM, Fitzmaurice TJ, Bujdoso R (2008) Copper- induced structural changes in the ovine prion protein are influenced by a polymorphism at codon 112. Biochim Biophys Acta 1784:683-692
6. Liang J, Pan Y, Zhang D, Guo C, Shi Y, Wang J, Chen Y, Wang X, Liu J, Guo X, Chen Z, Qiao T, Fan D (2007) Cellular prion protein promotes proliferation and G1/S transition of human gastric cancer cells SGC7901 and AGS. FASEB J 21:2247-2256 (Pubitemid 47026459)
7. Pokrovskii VI, Kiselev OI (1998) Molecular bases of prion diseases. Vestn Ross Akad Med Nauk 10:45-55
8. Liu ML, Li YX, Zhou XM, Zhao DM (2008) Copper(II) inhibits in vitro conformational conversion of ovine prion protein triggered by low pH. J Biochem 143:333-337
9. Weissmann C, Enari M, Klohn PC, Rossi D, Flechsig E (2002) Transmission of prions. J Infect Dis 186(Suppl 2):S157-S165 (Pubitemid 35370476)
10. Aguzzi A, Weissmann C (1997) Prion research: The next frontiers. Nature 389:795-798 (Pubitemid 27462961)
11. Chiesa R, Harris DA (2001) Prion diseases: What is the neurotoxic molecule? Neurobiol Dis 8:743-763
12. Saborio GP, Soto C, Kascsak RJ, Levy E, Kascsak R, Harris DA, Frangione B (1999) Cell-lysate conversion of prion protein into its protease-resistant isoform suggests the participation of a cellular chaperone. Biochem Biophys Res Commun 258:470-475 (Pubitemid 29287075)
13. Weiss S, Proske D, Neumann M, Groschup MH, Kretzschmar HA, Famulok M, Winnacker EL (1997) RNA aptamers specifically interact with the prion protein PrP. J Virol 71:8790-8797 (Pubitemid 27446463)
14. Zeiler B, Adler V, Kryukov V, Grossman A (2003) Concentration and removal of prion proteins from biological solutions. Biotechnol Appl Biochem 37:173-182 (Pubitemid 36399012)
15. Gomes MP, Millen TA, Ferreira PS, e Silva NL, Vieira TC, Almeida MS, Silva JL, Cordeiro Y (2008) Prion protein complexed to N2a cellular RNAs through its N-terminal domain forms aggregates and is toxic to murine neuroblastoma cells. J Biol Chem 283:19616-19625
16. Liu M, Yu S, Yang J, Yin X, Zhao D (2007) RNA and CuCl2 induced conformational changes of the recombinant ovine prion protein. Mol Cell Biochem 294:197-203 (Pubitemid 46188901)
17. Westphal D, Ledgerwood EC, Hibma MH, Fleming SB, Whelan EM, Mercer AA (2007) A novel Bcl-2-like inhibitor of apoptosis is encoded by the parapoxvirus ORF virus. J Virol 81:7178-7188 (Pubitemid 46986654)
18. Tomiyama A, Serizawa S, Tachibana K, Sakurada K, Samejima H, Kuchino Y, Kitanaka C (2006) Critical role for mitochondrial oxidative phosphorylation in the activation of tumor suppressors Bax and Bak. J Natl Cancer Inst 98:1462-1473 (Pubitemid 44605591)
19. Shibuta S, Kosaka J, Mashimo T, Fukuda Y, Yoshiya I (1998) Nitric oxide-induced cytotoxicity attenuation by thiopentone sodium but not pentobarbitone sodium in primary brain cultures. Br J Pharmacol 124:804-810 (Pubitemid 28276819)
20. Varathan S, Shibuta S, Varathan V, Takemura M, Yonehara N, Mashimo T (2003) Effects of deep hypothermia on nitric oxideinduced cytotoxicity in primary cultures of cortical neurons. J Neurosci Res 72:613-621 (Pubitemid 36578647)
21. Eghiaian F, Grosclaude J, Lesceu S, Debey P, Doublet B, Treguer E, Rezaei H, Knossow M (2004) Insight into the PrPC-[PrPSc conversion from the structures of antibody-bound ovine prion scrapie-susceptibility variants. Proc Natl Acad Sci USA 101: 10254-10259 (Pubitemid 38924912)
22. Wang C, Sadovova N, Ali HK, Duhart HM, Fu X, Zou X, Patterson TA, Binienda ZK, Virmani A, Paule MG, Slikker W Jr, Ali SF (2007) L-carnitine protects neurons from 1-methyl-4-phenylpyridinium- induced neuronal apoptosis in rat forebrain culture. Neuroscience 144:46-55 (Pubitemid 44848384)
23. Gupta R, Steward O (2003) Chronic nerve compression induces concurrent apoptosis and proliferation of Schwann cells. J Comp Neurol 461:174-186 (Pubitemid 36583461)
24. Sekiya T, Yagihashi A, Shimamura N, Asano K, Suzuki S, Matsubara A, Namba A, Shinkawa H (2003) Apoptosis of auditory neurons following central process injury. Exp Neurol 184:648-658 (Pubitemid 38182794)
25. Donne DG, Viles JH, Groth D, Mehlhorn I, James TL, Cohen FE, Prusiner SB, Wright PE, Dyson HJ (1997) Structure of the recombinant full-length hamster prion protein PrP(29-231): The N terminus is highly flexible. Proc Natl Acad Sci USA 94:13452-13457
26. Zou WQ, Cashman NR (2002) Acidic pH and detergents enhance in vitro conversion of human brain PrPC to a PrPSc-like form. J Biol Chem 277:43942-43947 (Pubitemid 36157818)
27. Nandi PK, Sizaret PY (2001) Murine recombinant prion protein induces ordered aggregation of linear nucleic acids to condensed globular structures. Arch Virol 146:327-345 (Pubitemid 32258301)
28. Horonchik L, Tzaban S, Ben-Zaken O, Yedidia Y, Rouvinski A, Papy-Garcia D, Barritault D, Vlodavsky I, Taraboulos A (2005) Heparan sulfate is a cellular receptor for purified infectious prions. J Biol Chem 280:17062-17067 (Pubitemid 41389169)
29. Cordeiro Y, Machado F, Juliano L, Juliano MA, Brentani RR, Foguel D, Silva JL (2001) DNA converts cellular prion protein into the beta-sheet conformation and inhibits prion peptide aggregation. J Biol Chem 276:49400-49409
30. Silva JL, Lima LM, Foguel D, Cordeiro Y (2008) Intriguing nucleic-acid-binding features of mammalian prion protein. Trends Biochem Sci 33:132-140
31. Cordeiro Y, Silva JL (2005) The hypothesis of the catalytic action of nucleic acid on the conversion of prion protein. Protein Pept Lett 12:251-255 (Pubitemid 40428415)
32. Baskakov IV, Legname G, Prusiner SB, Cohen FE (2001) Folding of prion protein to its native alpha-helical conformation is under kinetic control. J Biol Chem 276:19687-19690
33. Caughey B, Brown K, Raymond GJ, Katzenstein GE, Thresher W (1994) Binding of the protease-sensitive form of PrP (prion protein) to sulfated glycosaminoglycan and congo red [corrected]. J Virol 68:2135-2141 (Pubitemid 24091890)
34. S. Gilch, and H.M. Schatzl (2009) Aptamers against prion proteins and prions. Cell Mol Life Sci 35. Wang S, Lv X, Zhang K, Lin T, Liu X, Yuan J, Dai Y, Li N (2010) Knockdown of the prion gene expression by RNA interference in bovine fibroblast cells. Mol Biol Rep 37:3193-3198