Auxotrophic markers pyrF and proC can replace antibiotic markers on protein production plasmids in high-cell-density Pseudomonas fluorescens fermentation

Biotechnology Progress

Volumn 21, Issue 2, 2005, Pages 343-348

  Schneider, Jane C ^a   Jenings, Annika F ^a   Mun, Deborah M ^a   McGovern, Patricia M ^a,b   Chew, Lawrence C ^a  

^a DOW CHEMICAL COMPANY   (United States)

^b Ixion Biotechnology Inc   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBIOTICS; CELLS; FLUORESCENCE; GENES; MICROORGANISMS; PROTEINS;

ANTIBIOTIC RESISTANCE GENES; ANTIBIOTIC THERAPY; AUXOTROPHIC MARKERS; PATHOGENS;

BIOMARKERS;

ANTIINFECTIVE AGENT; BIOLOGICAL MARKER; PRIMER DNA;

ARTICLE; BACTERIAL GENE; FERMENTATION; GENE DELETION; METABOLISM; NUCLEOTIDE SEQUENCE; PLASMID; PSEUDOMONAS FLUORESCENS;

ANTI-BACTERIAL AGENTS; BASE SEQUENCE; BIOLOGICAL MARKERS; DNA PRIMERS; FERMENTATION; GENE DELETION; GENES, BACTERIAL; PLASMIDS; PSEUDOMONAS FLUORESCENS;

PSEUDOMONAS; PSEUDOMONAS FLUORESCENS;

EID: 16344374397     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1021/bp049696g     Document Type: Article

References (31)

1. Levy, S. B. Antibiotic availability and use: consequences to man and his environment. J. Clin. Epidemiol. 1991, 44, Suppl 2, 83S-87S.
2. Weigel, L. M.; Clewell, D. B.; Gill, S. R.; Clark, N. C.; McDougal, L. K.; Flannagan, S. E.; Kolonay, J. F.; Shetty, J.; Killgore, G. E.; Tenover, F. C. Genetic analysis of a high-level vancomycin-resistant isolate of Staphylococcus aureus. Science 2003, 302 (5650), 1569-1571.
3. Hanak, J. A. J.; Cranenburgh, R. M. Antibiotic-free plasmid selection and maintenance in bacteria. In Recombinant Protein Production with Prokaryotic and Eukaryotic Cells: A Comparative View on Host Physiology, Selected Articles from the Meeting of the EFB Section on Microbial Physiology, Semmering, Austria, Oct. 5-8, 2000; Merten, O.-W., Ed.; Kluwer Academic Publishers: Dordrecht, The Netherlands, 2001; pp 111-124.
4. Skogman, S. G.; Nilsson, J. Temperature-dependent retention of a tryptophan-operon-bearing plasmid in Escherichia coli. Gene 1984, 31, (1-3), 117-22.
5. Meinander, N. Q.; Hahn-Haegerdal, B. Fed-batch xylitol production with two recombinant Saccharomyces cerevisiae strains expressing XYL1 at different levels, using glucose as a cosubstrate: a comparison of production parameters and strain stability. Biotechnol Bioeng. 1997, 54 (4), 391-399.
6. Fiedler, M.; Skerra, A. proBA complementation of an auxotrophic E. coli strain improves plasmid stability and expression yield during fermenter production of a recombinant antibody fragment. Gene 2001, 274 (1-2), 111-118.
7. Degryse, E. Stability of a host-vector system based on complementation of an essential gene in Escherichia coli. J. Biotechnol. 1991, 18 (1-2), 29-39.
8. Boeke, J. D.; LaCroute, F.; Fink, G. R., A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance. Mol. Gen. Genet. 1984, 197, 345-346.
9. Sorensen, K. I.; Larsen, R.; Johansen, E. Cloning vectors not using antibiotic resistance markers for use in lactic acid bacteria in food processing. PCT Int. Appl. WO 9954488A1, 1999.
10. Lucas, S.; Toffin, L.; Zivanovic, Y.; Charlier, D.; Moussard, H.; Forterre, P.; Prieur, D.; Erauso, G. Construction of a shuttle vector for, and spheroplast transformation of, the hyperthermophilic archaeon Pyrococcus abyssi. Appl. Environmental Microbiol. 2002, 68 (11), 5528-5536.
11. Husson, R. N.; James, B. E.; Young, R. A. Gene replacement and expression of foreign DNA in mycobacteria. J. Bacteriol. 1990, 172 (2), 519-524.
12. Knipfer, N.; Seth, A.; Shrader, T. E. Unmarked gene integration into the chromosome of Mycobacterium smegmatis via precise replacement of the pyrF gene. Plasmid 1997, 37 (2), 129-140.
13. Bitan-Banin, G.; Ortenberg, R.; Mevarech, M. Development of a gene knockout system for the halophilic archaeon Haloferax volcanii by use of the pyrE gene. J. Bacteriol. 2003, 185 (3), 772-778.
14. Landry, T. D.; Chew, L.; Davis, J. W.; Frawley, N.; Foley, H. H.; Stelman, S. J.; Thomas, J.; Wolt, J.; Hanselman, D. S. Safety evaluation of an α-amylase enzyme preparation derived from the archaeal order Thermococcales as expressed in Pseudomonas fluorescens biovar I. Regulatory Toxicol. Pharmacol. 2003, 37 (1), 149-168.
15. Wilcox, E. R. Methods, vectors, and host cells for the control of expression of heterologous genes from lac operated promoters. U.S. Patent 5,169,760, 1992.
16. Stover, C. K.; Pham, X. Q.; Erwin, A. L.; Mizoguchi, S. D.; Warrener, P.; Hickey, M. J.; Brinkman, F. S. L.; Hufnagle, W. O.; Kowalik, J.; Lagrou, M.; Garber, R. L.; Goltry, L.; Tolentino, E.; Westbrock-Wadman, S.; Yuan, Y.; Brody, L. L.; Coulter, S. N.; Folger, K. R.; Kas, A.; Larbig, K.; Lim, R.; Smith, K.; Spencer, D.; Wong, G. K. S.; Wu, Z.; Paulsen, I. T.; Reizer, J.; Saier, M. H.; Hancock, R. E. W.; Lory, S.; Olson, M. V. Complete genome sequence of Pseudomonas aeruginosa PA01. Nature (London) 2000, 406 (6799), 959-964.
17. Artiguenave, F.; Vilagines, R.; Danglot, C. High-efficiency transposon mutagenesis by electroporation of a Pseudomonas fluorescens strain. FEMS Microbiol. Lett. 1997, 153 (2), 363-369.
18. Werle, E. Direct sequencing of polymerase chain reaction products. In Laboratory Methods for the Detection of Mutations and Polymorphisms in DNA; Taylor, G. R., Ed.; CRC Press: Boca Raton, FL, 1997; pp 163-174.
19. Fawcett, J. F.; Scott, J. H. A rapid and precise method for the determination of urea. J. Clin. Pathol. 1960, 13 (156), 156-159.
20. Richardson, T. H.; Tan, X.; Frey, G.; Callen, W.; Cabell, M.; Lam, D.; Macomber, J.; Short, J. M.; Robertson, D. E.; Miller, C. A novel, high performance enzyme for starch liquefaction: discovery and optimization of a low pH, thermostable a-amylase. J. Biol. Chem. 2002, 277 (29), 26501-26507.
21. Riesenberg, D.; Schulz, V.; Knorre, W. A.; Pohl, H. D.; Korz, D.; Sanders, E. A.; Ross, A.; Deckwer, W. D. High cell density cultivation of Escherichia coli at controlled specific growth rate. J. Biotechnol. 1991, 20 (1), 17-27.
22. DeSantis, G.; Wong, K.; Farwell, B.; Chatman, K.; Zhu, Z.; Tomlinson, G.; Huang, H.; Tan, X.; Bibbs, L.; Chen, P.; Kretz, K.; Burk, M. J. Creation of a productive, highly enantioselective nitrilase through gene site saturation mutagenesis (GSSM). J. Am. Chem. Soc. 2003, 125 (38), 11476-11477.
23. Wilcox, E. R.; Wong, S. Y. Translational enhancer for use in Pseudomonas. Eur. Pat. Appl. 91307500, 1992.
24. Toder, D. S. Gene replacement in Pseudomonas aeruginosa. Methods Enzymol. 1994, 235, 466-74.
25. Davison, J. Genetic tools for Pseudomonads, Rhizobia, and other gram-negative bacteria. BioTechniques 2002, 32 (2), 386-388, 390, 392-394, 396, 398-401.
26. Barik, A. Mutagenesis and Gene Fusion by Megaprimer PCR. In PCA Cloning Protocols; White, B. A., Ed.; Humana Press: Totowa, New Jersey, 1997; pp 173-182.
27. Nieto, C.; Fernandez-Tresguerres, E.; Sanchez, N.; Vicente, M.; Diaz, R. Cloning vectors, derived from a naturally occurring plasmid of Pseudomonas savastanoi, specifically tailored for genetic manipulations in Pseudomonas. Gene 1990, 87 (1), 145-149.
28. Strych, U.; Wohlfarth, S.; Winkler, U. K. Orotidine-5′- monophosphate decarboxylase from Pseudomonas aeruginosa PAO1: cloning, overexpression, and enzyme characterization. Curr. Microbiol. 1994, 29 (6), 353-359.
29. Zuker, M.; Mathews, D. H.; Turner, D. H. Algorithms and Thermodynamics for RNA Secondary Structure Prediction. In A Practical Guide in RNA Biochemistry and Biotechnology; Barciszewski, J., Clark, B. F. C., Eds.; Kluwer Academic Publishers: Norwell, MA, 1999; NATO ASI Series; pp 11-43.
30. Savioz, A; Jeenes, D. J.; Kocher, H.P.; Haas, D. Comparison of proC and other housekeeping genes of Pseudomonas aeruginosa with their counterparts in Escherichia coli. Gene 1990, 86, 107-111.
31. Womack, J. E.; O'Donovan, G. A. Orotic acid excretion in some wild-type strains of Escherichia coli K-12. J. Bacteriol. 1978, 136 (2), 825-828.