Generation of recombinant single-chain antibodies neutralizing the cytolytic activity of vaginolysin, the main virulence factor of Gardnerella vaginalis

BMC Biotechnology

Volumn 11, Issue , 2011, Pages

  Pleckaityte, Milda ^a   Mistiniene, Edita ^a   Lasickiene, Rita ^a   Zvirblis, Gintautas ^a   Zvirbliene, Aurelija ^a  

^a VILNIUS UNIVERSITY   (Lithuania)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIVE PROTEINS; ANTIGEN-BINDING ACTIVITIES; APPARENT K; CELL LYSIS; CYTOLYTIC ACTIVITIES; E. COLI; EXPRESSION SYSTEM; HELA CELL LINE; HEMOLYTIC ASSAYS; HUMAN ERYTHROCYTES; HYBRIDOMA CELLS; IN-VITRO; INCLUSION BODIES; LINKER SEQUENCE; MULTIVALENT BINDING; NEUTRALIZING ANTIBODIES; PROTEIN TOXINS; RECOMBINANT ANTIBODY; SINGLE-CHAIN ANTIBODIES; THERAPEUTIC AGENTS; VARIABLE FRAGMENT; VIRULENCE FACTORS;

BINDING ENERGY; CELL CULTURE; CELLS; CLONING; ESCHERICHIA COLI; METAL RECOVERY; PURIFICATION;

ANTIBODIES;

METAL CHELATE; NEUTRALIZING ANTIBODY; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; UNCLASSIFIED DRUG; VAGINOLYSIN; VIRULENCE FACTOR; BACTERIAL PROTEIN; BACTERIAL TOXIN; IMMUNOGLOBULIN FRAGMENT; RECOMBINANT PROTEIN; VAGINOLYSIN, GARDNERELLA VAGINALIS;

ARTICLE; CYTOLYSIS; ENZYME LINKED IMMUNOSORBENT ASSAY; ERYTHROCYTE; ESCHERICHIA COLI; GARDNERELLA VAGINALIS; GEL PERMEATION CHROMATOGRAPHY; HUMAN; HUMAN CELL; HYBRIDOMA; IMMUNOTHERAPY; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN EXPRESSION; PURIFICATION; BACTERIUM TRANSFORMATION; BIOSYNTHESIS; CELL INCLUSION; CELL SURVIVAL; CHEMISTRY; CYTOLOGY; DRUG EFFECT; FEMALE; GENETICS; HELA CELL; HEMOLYSIS; IMMUNOLOGY; MICROBIOLOGY; MOLECULAR CLONING; PLASMID; VAGINA; VAGINITIS;

BACTERIA (MICROORGANISMS); GARDNERELLA VAGINALIS; PROKARYOTA;

ANTIBODIES, NEUTRALIZING; BACTERIAL PROTEINS; BACTERIAL TOXINS; CELL SURVIVAL; CLONING, MOLECULAR; ENZYME-LINKED IMMUNOSORBENT ASSAY; ERYTHROCYTES; ESCHERICHIA COLI; FEMALE; GARDNERELLA VAGINALIS; HELA CELLS; HEMOLYSIS; HUMANS; HYBRIDOMAS; IMMUNOGLOBULIN FRAGMENTS; INCLUSION BODIES; PLASMIDS; RECOMBINANT PROTEINS; SINGLE-CHAIN ANTIBODIES; TRANSFORMATION, BACTERIAL; VAGINA; VAGINOSIS, BACTERIAL; VIRULENCE FACTORS;

EID: 80155170010     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-11-100     Document Type: Article

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