Antibody-based detection and inhibition of vaginolysin, the Gardnerella vaginalis cytolysin

PLoS ONE

Volumn 4, Issue 4, 2009, Pages

  Randis, Tara M ^a   Kulkarni, Ritwij ^a   Aguilar, Jorge L ^a   Ratner, Adam J ^a  

^a Och Spine at New York Presbyterian Hospitals   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTISERUM; BACTERIAL TOXIN; BACTERIUM ANTIBODY; CD59 ANTIGEN; CYTOLYSIN; PROTEIN ANTIBODY; UNCLASSIFIED DRUG; VAGINOLYSIN; ANTIBODY; BACTERIAL PROTEIN; DIAGNOSTIC AGENT; PERFORIN; VAGINOLYSIN, GARDNERELLA VAGINALIS;

ANTIBODY DETECTION; ARTICLE; BACTERIAL GROWTH; CARCINOMA CELL; CELL MIGRATION; CONTROLLED STUDY; CYTOLYSIS; EPITHELIUM CELL; ERYTHROCYTE; GARDNERELLA INFECTION; GARDNERELLA VAGINALIS; HEMOLYSIS; HUMAN; HUMAN CELL; NONHUMAN; PROTEIN DETERMINATION; PROTEIN PROTEIN INTERACTION; PROTEIN SYNTHESIS; UTERINE CERVIX CARCINOMA; VAGINA EPITHELIUM; ANIMAL; CELL DEATH; CELL LINE; DRUG ANTAGONISM; DRUG EFFECT; ENZYME LINKED IMMUNOSORBENT ASSAY; FEMALE; IMMUNOLOGY; ISOLATION AND PURIFICATION; METABOLISM; MICROBIOLOGY; PATHOGENICITY; RABBIT; TUMOR CELL LINE; UTERINE CERVIX TUMOR; VAGINA; VAGINITIS;

BACTERIA (MICROORGANISMS); GARDNERELLA VAGINALIS; ORYCTOLAGUS CUNICULUS;

ANIMALS; ANTIBODIES; ANTIGENS, CD59; BACTERIAL PROTEINS; BACTERIAL TOXINS; CELL DEATH; CELL LINE; CELL LINE, TUMOR; ENZYME-LINKED IMMUNOSORBENT ASSAY; ERYTHROCYTES; FEMALE; GARDNERELLA VAGINALIS; HEMOLYSIS; HUMANS; IMMUNE SERA; PERFORIN; RABBITS; UTERINE CERVICAL NEOPLASMS; VAGINA; VAGINOSIS, BACTERIAL;

EID: 65349155195     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0005207     Document Type: Article

References (28)

1. Eschenbach DA, Gravett MG, Chen KC, Hoyme UB, Holmes KK (1984) Bacterial vaginosis during pregnancy. An association with prematurity and postpartum complications. Scand J Urol Nephrol Suppl 86: 213-222.
2. Hillier SL, Nugent RP, Eschenbach DA, Krohn MA, Gibbs RS, et al. (1995) Association between bacterial vaginosis and preterm delivery of a low-birthweight infant. The Vaginal Infections and Prematurity Study Group. N Engl J Med 333: 1737-1742.
3. Watts DH, Eschenbach DA, Kenny GE (1989) Early postpartum endometritis: the role of bacteria, genital mycoplasmas, and Chlamydia trachomatis. Obstet Gynecol 73: 52-60.
4. Cohen CR, Duerr A, Pruithithada N, Rugpao S, Hillier S, et al. (1995) Bacterial vaginosis and HIV seroprevalence among female commercial sex workers in Chiang Mai, Thailand. Aids 9: 1093-1097.
5. Taha TE, Hoover DR, Dallabetta GA, Kumwenda NI, Mtimavalye LA, et al. (1998) Bacterial vaginosis and disturbances of vaginal flora: association with increased acquisition of HIV. Aids 12: 1699-1706.
6. Martin HL, Richardson BA, Nyange PM, Lavreys L, Hillier SL, et al. (1999) Vaginal lactobacilli, microbial flora, and risk of human immunodeficiency virus type 1 and sexually transmitted disease acquisition. J Infect Dis 180: 1863-1868.
7. Koumans EH, Kendrick JS (2001) Preventing adverse sequelae of bacterial vaginosis: a public health program and research agenda. Sex Transm Dis 28: 292-297.
8. Gardner HL, Dukes CD (1955) Haemophilus vaginalis vaginitis: a newly defined specific infection previously classified non-specific vaginitis. Am J Obstet Gynecol 69: 962-976.
9. Josey WE, Schwebke JR (2008) The polymicrobial hypothesis of bacterial vaginosis causation: a reassessment. Int J STD AIDS 19: 152-154.
10. Schwebke JR, Rivers C, Lee J (2008) Prevalence of Gardnerella vaginalis in Male Sexual Partners of Women With and Without Bacterial Vaginosis. Sex Transm Dis.
11. Gelber SE, Aguilar JL, Lewis KL, Ratner AJ (2008) Functional and phylogenetic characterization of Vaginolysin, the human-specific cytolysin from Gardnerella vaginalis. J Bacteriol 190: 3896-3903.
12. Fichorova RN, Rheinwald JG, Anderson DJ (1997) Generation of papillomavirus-immortalized cell lines from normal human ectocervical, endocervical, and vaginal epithelium that maintain expression of tissue-specific differentiation proteins. Biol Reprod 57: 847-855.
13. Hogan VK, Culhane JF, Hitti J, Rauh VA, McCollum KF, et al. (2007) Relative performance of three methods for diagnosing bacterial vaginosis during pregnancy. Matern Child Health J 11: 532-539.
14. Amsel R, Totten PA, Spiegel CA, Chen KC, Eschenbach D, et al. (1983) Nonspecific vaginitis. Diagnostic criteria and microbial and epidemiologic associations. Am J Med 74: 14-22.
15. Keane FE, Maw R, Pritchard C, Ison CA (2005) Methods employed by genitourinary medicine clinics in the United Kingdom to diagnose bacterial vaginosis. Sex Transm Infect 81: 155-157.
16. Nugent RP, Krohn MA, Hillier SL (1991) Reliability of diagnosing bacterial vaginosis is improved by a standardized method of gram stain interpretation. J Clin Microbiol 29: 297-301.
17. Tam MT, Yungbluth M, Myles T (1998) Gram stain method shows better sensitivity than clinical criteria for detection of bacterial vaginosis in surveillance of pregnant, low-income women in a clinical setting. Infect Dis Obstet Gynecol 6: 204-208.
18. Hedges SR, Barrientes F, Desmond RA, Schwebke JR (2006) Local and systemic cytokine levels in relation to changes in vaginal flora. J Infect Dis 193: 556-562.
19. Donder GG, Vereecken A, Bosmans E, Dekeersmaecker A, Salembier G, et al. (2002) Definition of a type of abnormal vaginal flora that is distinct from bacterial vaginosis: aerobic vaginitis. Bjog 109: 34-43.
20. Myziuk L, Romanowski B, Johnson SC (2003) BVBlue test for diagnosis of bacterial vaginosis. J Clin Microbiol 41: 1925-1928.
21. Calderon E, Rivera R, Gordillo S, Conde-Glez C (1997) Evaluation of a fast test to identify the presence of proline aminopeptidase in women with bacterial vaginosis. Infect Dis Obstet Gynecol 5: 226-231.
22. West B, Morison L, Schim van der Loeff M, Gooding E, Awasana AA, et al. (2003) Evaluation of a new rapid diagnostic kit (FemExam) for bacterial vaginosis in patients with vaginal discharge syndrome in The Gambia. Sex0 Transm Dis 30: 483-489.
23. Fredricks DN, Fiedler TL, Thomas KK, Mitchell CM, Marrazzo JM (2009) Changes in Vaginal Bacterial Concentrations with Intravaginal Metronidazole Therapy for Bacterial Vaginosis as Assessed by Quantitative PCR. J Clin Microbiol.
24. Menard JP, Fenollar F, Henry M, Bretelle F, Raoult D (2008) Molecular quantification of Gardnerella vaginalis and Atopobium vaginae loads to predict bacterial vaginosis. Clin Infect Dis 47: 33-43.
25. Briselden AM, Hillier SL (1994) Evaluation of affirm VP Microbial Identification Test for Gardnerella vaginalis and Trichomonas vaginalis. J Clin Microbiol 32: 148-152.
26. Nelson DB, Hanlon A, Hassan S, Britto J, Geifman-Holtzman O, et al. (2008) Preterm labor and bacterial vaginosis-associated bacteria among urban women. J Perinat Med.
27. Klein LL, Gibbs RS (2004) Use of microbial cultures and antibiotics in the prevention of infection-associated preterm birth. Am J Obstet Gynecol 190: 1493-1502.
28. McDonald HM, Brocklehurst P, Gordon A (2007) Antibiotics for treating bacterial vaginosis in pregnancy. Cochrane Database Syst Rev. CD000262.