Characterization and modulation of fibroblast/endothelial cell co-cultures for the in vitro preformation of threedimensional tubular networks

Cell Biology International

Volumn 35, Issue 11, 2011, Pages 1097-1110

  Eckermann, Christoph W ^a,b   Lehle, Karla ^c   Schmid, Stephan A ^b,c   Wheatley, Denys N ^d   Kunz Schughart, Leoni A ^a  

^a DRESDEN UNIVERSITY OF TECHNOLOGY   (Germany)

^b UNIVERSITY OF REGENSBURG   (Germany)

^c UNIVERSITY HOSPITAL REGENSBURG   (Germany)

^d BioMedES   (United Kingdom)

Author keywords

Fibroblast; Human umbilical vein endothelial cells (HUVEC); Microvascular endothelial cell; Multilayer co culture; Spheroid co culture

Indexed keywords

EPIDERMAL GROWTH FACTOR; FIBROBLAST GROWTH FACTOR 2; SOMATOMEDIN C; VASCULOTROPIN;

ANGIOGENESIS; ARTICLE; CELL COUNT; CELL DEATH; CELL FUNCTION; CELL SIZE; CELL SURVIVAL; CELL VIABILITY; COCULTURE; CONTROLLED STUDY; CULTURE MEDIUM; FIBROBLAST CULTURE; HUMAN; HUMAN CELL; IN VITRO STUDY; INOCULATION; MICROVASCULAR ENDOTHELIAL CELL; UMBILICAL VEIN ENDOTHELIAL CELL;

APOPTOSIS; CELL COUNT; CELL SURVIVAL; CELLS, CULTURED; COCULTURE TECHNIQUES; CULTURE MEDIA; DNA FRAGMENTATION; FIBROBLAST GROWTH FACTORS; FIBROBLASTS; HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS; HUMANS; NEOVASCULARIZATION, PHYSIOLOGIC; SERUM; SPHEROIDS, CELLULAR; TIME FACTORS; TISSUE ENGINEERING; VASCULAR ENDOTHELIAL GROWTH FACTOR A;

BOVINAE;

EID: 80054971741     PISSN: 10656995     EISSN: 10958355     Source Type: Journal    
DOI: 10.1042/CBI20100718     Document Type: Article

References (63)

1. Aird WC. Phenotypic heterogeneity of the endothelium: I. Structure, function, and mechanisms. Circ Res 2007a;100:158-73.
2. Aird WC. Phenotypic heterogeneity of the endothelium: II. Representative vascular beds. Circ Res 2007b;100:174-90.
3. Alajati A, Laib AM, Weber H, Boos AM, Bartol A, Ikenberg K et al. Spheroid-based engineering of a human vasculature in mice. Nat Meth 2008;5:439-45.
4. Alexander TH, Sage AB, Schumacher BL, Sah RL, Watson D. Human serum for tissue engineering of human nasal septal cartilage. Otolaryngol Head Neck Surg 2006;135:397-403.
5. Ali-Bahar M, Bauer B, Tredget EE, Ghahary A. Dermal fibroblasts from different layers of human skin are heterogeneous in expression of collagenase and types I and III procollagen mRNA. Wound Repair Regen 2004;12:175-82.
6. Arnqvist HJ. The role of IGF-system in vascular insulin resistance. Horm Metab Res 2008;40:588-92.
7. Auerbach R, Akhtar N, Lewis RL, Shinners BL. Angiogenesis assays: problems and pitfalls. Cancer Metastasis Rev 2000;19:167-72.
8. Auerbach R, Lewis R, Shinners B, Kubai L, Akhtar N. Angiogenesis assays: a critical overview. Clin Chem 2003;49:32-40.
9. Bao P, Kodra A, Tomic-Canic M, Golinko MS, Ehrlich HP, Brem H. The role of vascular endothelial growth factor in wound healing. J Surg Res 2009;153:347-58.
10. Beckert S, Farrahi F, Perveen Ghani Q, Aslam R, Scheuenstuhl H, Coerper S et al. IGF-I-induced VEGF expression in HUVEC involves phosphorylation and inhibition of poly(ADP-ribose)polymerase. Biochem Biophys Res Commun 2006;341:67-72.
11. Bishop ET, Bell GT, Bloor S, Broom IJ, Hendry NF, Wheatley DN. An in vitro model of angiogenesis: basic features. Angiogenesis 1999;3:335-44.
12. Boisen L, Drasbek KR, Pedersen AS, Kristensen P. Evaluation of endothelial cell culture as a model system of vascular ageing. Exp Gerontol 2010;45:779-87.
13. Chisalita SI, Arnqvist HJ. Insulin-like growth factor I receptors are more abundant than insulin receptors in human micro- and macrovascular endothelial cells. Am J Physiol Endocrinol Metabol 2004;286:E896-901.
14. Cristofalo VJ. Cell culture aging: insights for cell aging in vivo? Aging (Milano) 1999;11:1-3.
15. Cristofalo VJ, Lorenzini A, Allen RG, Torres C, Tresini M. Replicative senescence: a critical review. Mech Ageing Dev 2004;125:827-48.
16. Cristofalo VJ, Volker C, Francis MK, Tresini M. Age-dependent modifications of gene expression in human fibroblasts. Crit Rev Eukaryot Gene Expr 1998;8:43-80.
17. Delafontaine P, Song YH, Li Y. Expression, regulation, and function of IGF-1, IGF-1R, and IGF-1 binding proteins in blood vessels. Arterioscler Thromb Vasc Biol 2004;24:435-44.
18. Distler JH, Hirth A, Kurowska-Stolarska M, Gay RE, Gay S, Distler O. Angiogenic and angiostatic factors in the molecular control of angiogenesis. Q J Nucl Med 2003;47:149-61.
19. Donnini S, Solito R, Cetti E, Corti F, Giachetti A, Carra S et al. Abeta peptides accelerate the senescence of endothelial cells in vitro and in vivo, impairing angiogenesis. FASEB J 2010;24:2385-95.
20. Donovan D, Brown NJ, Bishop ET, Lewis CE. Comparison of three in vitro human 'angiogenesis' assays with capillaries formed in vivo. Angiogenesis 2001;4:113-21.
21. Ferrara N. VEGF-A: a critical regulator of blood vessel growth. Eur Cytokine Network 2009;20:158-63.
22. Finkenzeller G, Graner S, Kirkpatrick CJ, Fuchs S, Stark GB. Impaired in vivo vasculogenic potential of endothelial progenitor cells in comparison to human umbilical vein endothelial cells in a spheroid- based implantation model. Cell Prolif 2009;42:498-505.
23. Finkenzeller G, Torio-Padron N, Momeni A, Mehlhorn AT, Stark GB. In vitro angiogenesis properties of endothelial progenitor cells: a promising tool for vascularization of ex vivo engineered tissues. Tissue Eng 2007;13:1413-20.
24. French LE, Hahne M, Viard I, Radlgruber G, Zanone R, Becker K et al. Fas and Fas ligand in embryos and adult mice: ligand expression in several immune-privileged tissues and coexpression in adult tissues characterized by apoptotic cell turnover. J Cell Biol 1996;133: 335-43.
25. Goodwin AM. In vitro assays of angiogenesis for assessment of angiogenic and anti-angiogenic agents. Microvasc Res 2007;74:172-83.
26. Hewett PW. Vascular endothelial cells from human micro- and macrovessels: isolation, characterisation and culture. Methods Mol Biol 2009;467:95-111.
27. Jaffé EA, Nachman RL, Becker CG, Minick CR. Culture of human endothelial cells derived from umbilical veins. Identification by morphologic and immunologic criteria. J Clin Invest 1973;52:2745-56.
28. Kocaoemer A, Kern S, Kluter H, Bieback K. Human AB serum and thrombin-activated platelet-rich plasma are suitable alternatives to fetal calf serum for the expansion of mesenchymal stem cells from adipose tissue. Stem Cells 2007;25:1270-8.
29. Korff T, Augustin HG. Integration of endothelial cells in multicellular spheroids prevents apoptosis and induces differentiation. J Cell Biol 1998;143:1341-52.
30. Korff T, Augustin HG. Tensional forces in fibrillar extracellular matrices control directional capillary sprouting. J Cell Sci 1999;112:3249-58.
31. Korff T, Kimmina S, Martiny-Baron G, Augustin HG. Blood vessel maturation in a 3-dimensional spheroidal coculture model: direct contact with smooth muscle cells regulates endothelial cell quiescence and abrogates VEGF responsiveness. FASEB J 2001;15:447-57.
32. Kunz-Schughart LA, Heyder P, Schroeder J, Knuechel R. A heterologous 3-D coculture model of breast tumor cells and fibroblasts to study tumor-associated fibroblast differentiation. Exp Cell Res 2001;266:74-86.
33. Kunz-Schughart LA, Wenninger S, Neumeier T, Seidl P, Knuechel R. Three-dimensional tissue structure affects sensitivity of fibroblasts to TGF-beta 1. Am J Physiol Cell Physiol 2003;284:C209-19.
34. Kunz-Schughart LA, Schroeder JA, Wondrak M, van RF, Lehle K, Hofstaedter F, Wheatley DN. Potential of fibroblasts to regulate the formation of three-dimensional vessel-like structures from endothelial cells in vitro. Am J Physiol Cell Physiol 2006;290:C1385-98.
35. Labitzke R, Friedl P. A serum-free medium formulation supporting growth of human umbilical cord vein endothelial cells in long-term cultivation. Cytotechnology 2001;35:87-92.
36. Laschke MW, Harder Y, Amon M, Martin I, Farhadi J, Ring A et al. Angiogenesis in tissue engineering: breathing life into constructed tissue substitutes. Tissue Eng 2006;12:2093-104.
37. Lehle K, Kunz-Schughart LA, Kuhn P, Schreml S, Birnbaum DE, Preuner JG. Validity of a patient-derived system of tissue-specific human endothelial cells: interleukin-6 as a surrogate marker in the coronary system. Am J Physiol Heart Circ Physiol 2007;293:H1721-8.
38. Lehle K, Straub RH, Morawietz H, Kunz-Schughart LA. The relevance of disease- and organ-specific endothelial cells for in vitro research. Cell Biol Int 2010;34:1231-8.
39. Leung BM, Sefton MV. A modular tissue engineering construct containing smooth muscle cells and endothelial cells. Ann Biomed Eng 2007;35:2039-49.
40. Levenberg S, Rouwkema J, Macdonald M, Garfein ES, Kohane DS, Darland DC et al. Engineering vascularized skeletal muscle tissue. Nat Biotechnol 2005;23:879-84.
41. Ma QL, Yang TL, Yin JY, Peng ZY, Yu M, Liu ZQ, Chen FP. Role of insulin-like growth factor-1 (IGF-1) in regulating cell cycle progression. Biochem Biophys Res Commun 2009;389:150-5.
42. Maharaj AS, D'Amore PA. Roles for VEGF in the adult. Microvasc Res 2007;74:100-13.
43. McGuigan AP, Sefton MV. Vascularized organoid engineered by modular assembly enables blood perfusion. Proc Natl Acad Sci USA 2006;103:11461-6.
44. Methe H, Edelman ER. Tissue engineering of endothelial cells and the immune response. Transplant Proc 2006;38:3293-9.
45. Moon JJ, Lee SH, West JL. Synthetic biomimetic hydrogels incorporated with ephrin-A1 for therapeutic angiogenesis. Biomacromolecules 2007;8:42-9.
46. Moon JJ, West JL. Vascularization of engineered tissues: approaches to promote angio-genesis in biomaterials. Curr Top Med Chem 2008;8:300-10.
47. Murakami M, Nguyen LT, Zhuang ZW, Moodie KL, Carmeliet P, Stan RV, Simons M. The FGF system has a key role in regulating vascular integrity. J Clin Invest 2008;118:3355-66.
48. Murakami M, Simons M. Fibroblast growth factor regulation of neovascularization. Current Opin Hematol 2008;15:215-20.
49. Nakao-Hayashi J, Ito H, Kanayasu T, Morita I, Murota S. Stimulatory effects of insulin and insulin-like growth factor I on migration and tube formation by vascular endothelial cells. Atherosclerosis 1992;92:141-9.
50. Nicosia RF, Nicosia SV, Smith M. Vascular endothelial growth factor, platelet-derived growth factor, and insulin-like growth factor-1 promote rat aortic angiogenesis in vitro. Am J Pathol 1994;145:1023-9.
51. Nieves BJ, D'Amore PA, Bryan BA. The function of vascular endothelial growth factor. Biofactors 2009;35:332-7.
52. Presta M, Dell'Era P, Mitola S, Moroni E, Ronca R, Rusnati M. Fibroblast growth factor/fibroblast growth factor receptor system in angiogenesis. Cytokine Growth Factor Rev 2005;16:159-78.
53. Ribatti D, Nico B, Vacca A, Roncali L, Dammacco F. Endothelial cell heterogeneity and organ specificity. J Hematother Stem Cell Res 2002;11:81-90.
54. Rivron NC, Liu JJ, Rouwkema J, de Boer J, van Blitterswijk CA. Engineering vascularised tissues in vitro. Eur Cell Mater J 2008;15:27-40.
55. Rubin H. Cell aging in vivo and in vitro. Mech Ageing Dev 1997;98:1-35.
56. Schmid SA, Gaumann A, Wondrak M, Eckermann C, Schulte S, Mueller-Klieser W et al. Lactate adversely affects the in vitro formation of endothelial cell tubular structures through the action of TGF-beta1. Exp Cell Res 2007;313:2531-49.
57. Shigematsu S, Yamauchi K, Nakajima K, Iijima S, Aizawa T, Hashizume K. D-Glucose and insulin stimulate migration and tubular formation of human endothelial cells in vitro. Am J Physiol 1999a;277:E433-8.
58. Shigematsu S, Yamauchi K, Nakajima K, Iijima S, Aizawa T, Hashizume K. IGF-1 regulates migration and angiogenesis of human endothelial cells. Endocrine J 1999b;46 (Suppl):S59-62.
59. Staton CA, Reed MW, Brown NJ. A critical analysis of current in vitro and in vivo angiogenesis assays. Int J Exp Pathol 2009;90:195-221.
60. Tateishi K, Ando W, Higuchi C, Hart DA, Hashimoto J, Nakata K et al. Comparison of human serum with fetal bovine serum for expansion and differentiation of human synovial MSC: potential feasibility for clinical applications. Cell Transplant 2008;17:549-57.
61. Trkov S, Eng G, Di Liddo R, Parnigotto PP, Vunjak-Novakovic G. Micropatterned three-dimensional hydrogel system to study human endothelial-mesenchymal stem cell interactions. J Tissue Eng Regen Med 2010;4:205-15.
62. Tsigkou O, Pomerantseva I, Spencer JA, Redondo PA, Hart AR, O'Doherty E et al. Engineered vascularized bone grafts. Proc Natl Acad Sci USA 2010;107:3311-6.
63. van Cruijsen H, Giaccone G, Hoekman K. Epidermal growth factor receptor and angiogenesis: opportunities for combined anticancer strategies. Int J Cancer 2005;117:883-8.