A serum-free medium formulation supporting growth of human umbilical cord vein endothelial cells in long-term cultivation

Cytotechnology

Volumn 35, Issue 2, 2001, Pages 87-92

  Labitzke, Regina ^a   Friedl, Peter ^a  

^a DARMSTADT UNIVERSITY OF TECHNOLOGY   (Germany)

Author keywords

Cells; Cultured; Endothelium; Serum free medium

Indexed keywords

ACETYLCYSTEINE; CELL GROWTH; CULTURE MEDIUM; ENDOTHELIUM CELL; GLUTAMINE; HUMAN UMBILICAL CORD VEIN; HYDROCORTISONE; INSULIN; PHOSPHOASCORBIC ACID; SERINE;

EID: 0034789411     PISSN: 09209069     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1017551218007     Document Type: Article

References (15)

1. The proliferation of human umbilical vein endothelial cells in serum-free medium
2. An optimized culture medium for human vascular endothelial cells from umbilical cord veins
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4. Isolation and primary culture of endothelial cells from human umbilical vessels
5. Growth and physiological functions of vascular endothelial cells in a new serum-free medium (SFM)
6. The role of fibroblast growth factor and the extracellular matrix in the control of proliferation and differentiation of corneal endothelial cells
7. Brain- and liver cell-derived factors are required for growth of human endothelial cells in serum-free culture
8. Culture of human endothelial cells derived from umbilical veins. Identification by morphologic and immunologic criteria
9. Serum-free growth medium for the cultivation of a wide spectrum of mammalian cells in a stirred bioreactor
10. Optimized medium for clonal growth of human microvascular endothelial cells with minimal serum
11. Serial propagation of human endothelial cells in vitro
12. Metabolic rates of endothelial cells in vitro
13. Preservation of differentiated phenotypes in cultured aortic endothelial cells by malotilate and phosphoascorbic acid
14. Vascular endothelial cells maintained in the absence of fibroblast growth factor undergo structural and functional alterations that are incompatible with their in vivo differentiated properties
15. Serial subculture and relative transport of human endothelial cells in serum-free, defined conditions