Directed evolution of a pyruvate aldolase to recognize a long chain acyl substrate

Bioorganic and Medicinal Chemistry

Volumn 19, Issue 21, 2011, Pages 6447-6453

  Cheriyan, Manoj ^a   Walters, Matthew J ^b   Kang, Brian D ^a   Anzaldi, Laura L ^c   Toone, Eric J ^b,c   Fierke, Carol A ^a,d  

^a UNIVERSITY OF MICHIGAN   (United States)

^b DUKE UNIVERSITY MEDICAL CENTER   (United States)

^c DUKE UNIVERSITY   (United States)

^d UNIVERSITY OF MICHIGAN MEDICAL SCHOOL   (United States)

Author keywords

Biocatalysis; Protein engineering; Random mutagenesis; Substrate specificity; Thermostable enzyme

Indexed keywords

2 KETO 3 DEOXY 6 PHOSPHOGLUCONATE ALDOLASE; FRUCTOSE BISPHOSPHATE ALDOLASE; PYRUVIC ACID; UNCLASSIFIED DRUG;

ARTICLE; AUXOTROPHY; BACTERIAL GENOME; CATALYSIS; CONTROLLED STUDY; ELECTROPHILICITY; ESCHERICHIA COLI; GENE MUTATION; IN VIVO STUDY; NONHUMAN; PROTEIN CLEAVAGE; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN STRUCTURE; THERMOTOGA MARITIMA;

ESCHERICHIA COLI; THERMOTOGA MARITIMA;

EID: 80054915273     PISSN: 09680896     EISSN: 14643391     Source Type: Journal    
DOI: 10.1016/j.bmc.2011.08.056     Document Type: Article

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