Development and inter-laboratory validation of unlabeled probe melting curve analysis for detection of JAK2 V617F mutation in polycythemia vera

PLoS ONE

Volumn 6, Issue 10, 2011, Pages

  Wu, Zhiyuan ^a   Yuan, Hong ^b   Zhang, Xinju ^a   Liu, Weiwei ^a   Xu, Jinhua ^a   Zhang, Wei ^c   Guan, Ming ^a  

^a FUDAN UNIVERSITY   (China)

^b DALIAN MEDICAL UNIVERSITY   (China)

^c Biomedical Research Institute   (China)

Author keywords

[No Author keywords available]

Indexed keywords

PHENYLALANINE; VALINE; JANUS KINASE 2;

ARTICLE; CONTROLLED STUDY; GENE; GENOTYPE; HUMAN; HUMAN CELL; INTERMETHOD COMPARISON; JAK2 GENE; MELTING CURVE ANALYSIS; MISSENSE MUTATION; MOLECULAR PROBE; MUTATIONAL ANALYSIS; NUCLEIC ACID ANALYSIS; NUCLEOTIDE SEQUENCE; POLYCYTHEMIA VERA; POLYMERASE CHAIN REACTION; PROCESS DEVELOPMENT; SENSITIVITY AND SPECIFICITY; VALIDATION STUDY; CHEMISTRY; DNA DENATURATION; DNA PROBE; ENZYMOLOGY; GENETICS; INSTRUMENTATION; LABORATORY; METHODOLOGY; NUCLEIC ACID AMPLIFICATION; TRANSITION TEMPERATURE; TUMOR CELL LINE;

CELL LINE, TUMOR; DNA MUTATIONAL ANALYSIS; DNA PROBES; HUMANS; JANUS KINASE 2; LABORATORIES; MUTATION, MISSENSE; NUCLEIC ACID AMPLIFICATION TECHNIQUES; NUCLEIC ACID DENATURATION; POLYCYTHEMIA VERA; TRANSITION TEMPERATURE;

EID: 80054777529     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0026534     Document Type: Article

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