High-throughput genotyping of single nucleotide polymorphisms in the Plasmodium falciparum dhfr gene by asymmetric PCR and melt-curve analysis

Journal of Clinical Microbiology

Volumn 48, Issue 9, 2010, Pages 3081-3087

  Cruz, Rochelle E ^a   Shokoples, Sandra E ^b   Manage, Dammika P ^a   Yanow, Stephanie K ^a,b  

^a UNIVERSITY OF ALBERTA   (Canada)

^b PROVINCIAL LABORATORY FOR PUBLIC HEALTH   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

GENOMIC DNA; PROTEIN DHFR; PROTOZOAL PROTEIN; PYRIMETHAMINE; REAGENT; UNCLASSIFIED DRUG;

AMPLICON; ARTICLE; CANADA; CLINICAL ARTICLE; CONTROLLED STUDY; DNA SEQUENCE; DNA TEMPLATE; GENE NUMBER; GENE SEQUENCE; GENETIC ANALYSIS; GENOTYPE; HIGH THROUGHPUT SCREENING; HUMAN; IMMIGRANT; IMMUNOASSAY; IN SITU HYBRIDIZATION; LIGHT CHAIN; MALARIA FALCIPARUM; MELT CURVE ANALYSIS; MOLECULAR PROBE; PARASITE ISOLATION; PLASMID; PLASMODIUM FALCIPARUM; PRIORITY JOURNAL; PROTOZOAL GENETICS; PYROSEQUENCING; REACTION ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SINGLE NUCLEOTIDE POLYMORPHISM; WILD TYPE;

DNA, PROTOZOAN; DRUG RESISTANCE; GENOTYPE; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; MALARIA, FALCIPARUM; PARASITIC SENSITIVITY TESTS; PLASMODIUM FALCIPARUM; POLYMERASE CHAIN REACTION; POLYMORPHISM, SINGLE NUCLEOTIDE; PROTOZOAN PROTEINS; SENSITIVITY AND SPECIFICITY; TETRAHYDROFOLATE DEHYDROGENASE; TRANSITION TEMPERATURE;

PLASMODIUM FALCIPARUM;

EID: 77956847296     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.00634-10     Document Type: Article

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