Human hepatic stellate cell line (LX-2) exhibits characteristics of bone marrow-derived mesenchymal stem cells

Experimental and Molecular Pathology

Volumn 91, Issue 3, 2011, Pages 664-672

  Castilho Fernandes, Andrielle ^a,b   de Almeida, Danilo Candido ^a,b   Fontes, Aparecida Maria ^a,b   Melo, Fernanda Ursoli Ferreira ^b   Picanço Castro, Virgínia ^b   Freitas, Marcela Cristina ^a,b   Orellana, Maristela D ^a,b   Palma, Patricia V B ^b   Hackett, Perry B ^c   Friedman, Scott L ^d   Covas, Dimas Tadeu ^a,b  

^a UNIVERSITY OF SÃO PAULO   (Brazil)

^b National Institute of Science and Technology in Stem Cell and Cell Therapy   (Brazil)

^c UNIVERSITY OF MINNESOTA   (United States)

^d MOUNT SINAI SCHOOL OF MEDICINE   (United States)

Author keywords

Hepatic stellate cell line; LX 2; Mesenchymal stem cells; Pericytes

Indexed keywords

5' NUCLEOTIDASE; ACTIVATED LEUKOCYTE CELL ADHESION MOLECULE; ALPHA5 INTEGRIN; BETA1 INTEGRIN; CD146 ANTIGEN; CD271 ANTIGEN; CD31 ANTIGEN; CD34 ANTIGEN; CD45 ANTIGEN; CELL MARKER; COLLAGEN TYPE 1; ENDOGLIN; HERMES ANTIGEN; HLA ANTIGEN CLASS 1; LIPOCORTIN 5; MICROSOMAL AMINOPEPTIDASE; NESTIN; PLATELET DERIVED GROWTH FACTOR BETA RECEPTOR; THY 1 ANTIGEN; UNCLASSIFIED DRUG; VIMENTIN;

ADIPOCYTE; ANGIOGENESIS; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BONE MARROW CELL; CARCINOGENIC ACTIVITY; CELL ACTIVATION; CELL DIFFERENTIATION; CELL FUNCTION; CELL INTERACTION; CELL LINE; CELL STIMULATION; CELL STRUCTURE; CONTROLLED STUDY; FEMALE; HUMAN; HUMAN CELL; IMMUNOPHENOTYPING; IN VITRO STUDY; IN VIVO STUDY; MESENCHYMAL STEM CELL; MOUSE; NONHUMAN; OSTEOCYTE; PHENOTYPE; PROTEIN EXPRESSION; STELLATE CELL;

ANIMALS; ANTIGENS, CD; BONE MARROW CELLS; CELL DIFFERENTIATION; CELL LINE; CELL TRANSPLANTATION; HEPATIC STELLATE CELLS; HUMANS; IMMUNOPHENOTYPING; MESENCHYMAL STEM CELLS; MICE; MICE, SCID; MULTIPOTENT STEM CELLS; NEOPLASMS, EXPERIMENTAL; NEOVASCULARIZATION, PATHOLOGIC; OSTEOGENESIS;

EID: 80053505418     PISSN: 00144800     EISSN: 10960945     Source Type: Journal    
DOI: 10.1016/j.yexmp.2011.09.002     Document Type: Article

References (62)

1. Albesiano E., et al. Activation-induced cytidine deaminase in chronic lymphocytic leukemia B cells: expression as multiple forms in a dynamic, variably sized fraction of the clone. Blood 2003, 102:3333-3339.
2. Alison M.R., et al. Application of liver stem cells for cell therapy. Seminars in Cell & Developmental Biology 2007, 18:819-826.
3. Ankoma-Sey V., et al. Coordinated induction of VEGF receptors in mesenchymal cell types during rat hepatic wound healing. Oncogene 1998, 17:115-121.
4. Armulik A., et al. Endothelial/pericyte interactions. Circulation Research 2005, 97:512-523.
5. Arthur M.J., et al. Tissue inhibitors of metalloproteinases: role in liver fibrosis and alcoholic liver disease. Alcoholism, Clinical and Experimental Research 1999, 23:940-943.
6. Asahina K., et al. Mesenchymal origin of hepatic stellate cells, submesothelial cells, and perivascular mesenchymal cells during mouse liver development. Hepatology 2009, 49:998-1011.
7. Atzori L., et al. Hepatic stellate cell: a star cell in the liver. The International Journal of Biochemistry & Cell Biology 2009, 41:1639-1642.
8. Benyon R.C., Arthur M.J. Extracellular matrix degradation and the role of hepatic stellate cells. Seminars in Liver Disease 2001, 21:373-384.
9. Bernardo M.E., et al. Human bone marrow derived mesenchymal stem cells do not undergo transformation after long-term in vitro culture and do not exhibit telomere maintenance mechanisms. Cancer Research 2007, 67:9142-9149.
10. Bhattacharya R., et al. Regulatory role of dynamin-2 in VEGFR-2/KDR-mediated endothelial signaling. The FASEB Journal 2005, 19:1692-1694.
11. Bordeaux-Rego P., et al. Both interleukin-3 and interleukin-6 are necessary for better ex vivo expansion of CD133+ cells from umbilical cord blood. Stem Cells and Development 2010, 19:413-422.
12. Brachvogel B., et al. Perivascular cells expressing annexin A5 define a novel mesenchymal stem cell-like population with the capacity to differentiate into multiple mesenchymal lineages. Development 2005, 132:2657-2668.
13. Brenner D.A., et al. New aspects of hepatic fibrosis. Journal of Hepatology 2000, 32:32-38.
14. Budny T., et al. Morphologic features in the regenerating liver - a comparative intravital, lightmicroscopical and ultrastructural analysis with focus on hepatic stellate cells. Virchows Archiv 2007, 451:781-791.
15. Bustin S.A., et al. The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clinical Chemistry 2009, 55:611-622.
16. Carotti S., et al. Glial fibrillary acidic protein as an early marker of hepatic stellate cell activation in chronic and posttransplant recurrent hepatitis C. Liver Transplantation 2008, 14:806-814.
17. Carpino G., et al. Alpha-SMA expression in hepatic stellate cells and quantitative analysis of hepatic fibrosis in cirrhosis and in recurrent chronic hepatitis after liver transplantation. Digestive and Liver Disease 2005, 37:349-356.
18. Cassiman D., et al. Hepatic stellate cell/myofibroblast subpopulations in fibrotic human and rat livers. Journal of Hepatology 2002, 36:200-209.
19. Choumerianou D.M., et al. Study of oncogenic transformation in ex vivo expanded mesenchymal cells, from paediatric bone marrow. Cell Proliferation 2008, 41:909-922.
20. Covas D.T., et al. Multipotent mesenchymal stromal cells obtained from diverse human tissues share functional properties and gene-expression profile with CD146+ perivascular cells and fibroblasts. Experimental Hematology 2008, 36:642-654.
21. De Minicis S., et al. Gene expression profiles during hepatic stellate cell activation in culture and in vivo. Gastroenterology 2007, 132:1937-1946.
22. Deans R.J., Moseley A.B. Mesenchymal stem cells: biology and potential clinical uses. Experimental Hematology 2000, 28:875-884.
23. Flohr T.R., et al. The use of stem cells in liver disease. Current Opinion in Organ Transplantation 2009, 14:64-71.
24. Friedman S.L. Molecular regulation of hepatic fibrosis, an integrated cellular response to tissue injury. The Journal of Biological Chemistry 2000, 275:2247-2250.
25. Friedman S.L. Hepatic stellate cells: protean, multifunctional, and enigmatic cells of the liver. Physiological Reviews 2008, 88:125-172.
26. Friedman S.L., et al. Hepatic lipocytes: the principal collagen-producing cells of normal rat liver. Proceedings of the National Academy of Sciences of the United States of America 1985, 82:8681-8685.
27. Fujii T., et al. Mouse model of carbon tetrachloride induced liver fibrosis: histopathological changes and expression of CD133 and epidermal growth factor. BMC Gastroenterology 2010, 10:79.
28. Galli A., et al. Antidiabetic thiazolidinediones inhibit collagen synthesis and hepatic stellate cell activation in vivo and in vitro. Gastroenterology 2002, 122:1924-1940.
29. Gaudio E., et al. New insights into liver stem cells. Digestive and Liver Disease 2009, 41:455-462.
30. Gruber R., et al. Bone marrow stromal cells can provide a local environment that favors migration and formation of tubular structures of endothelial cells. Tissue Engineering 2005, 11:896-903.
31. Herrmann J., et al. Immortal hepatic stellate cell lines: useful tools to study hepatic stellate cell biology and function?. Journal of Cellular and Molecular Medicine 2007, 11:704-722.
32. Ishikawa K., et al. Expressions of vascular endothelial growth factor in nonparenchymal as well as parenchymal cells in rat liver after necrosis. Biochemical and Biophysical Research Communications 1999, 254:587-593.
33. Ito T., Shibasaki S. Electron microscopic study on the hepatic sinusoidal wall and the fat-storing cells in the normal human liver. Archivum Histologicum Japonicum 1968, 29:137-192.
34. Kang T.J., et al. Growth kinetics of human mesenchymal stem cells from bone marrow and umbilical cord blood. Acta Haematologica 2004, 112:230-233.
35. Karp J.M., Leng Teo G.S. Mesenchymal stem cell homing: the devil is in the details. Cell Stem Cell 2009, 4:206-216.
36. Katritsis D.G., et al. Transcoronary transplantation of autologous mesenchymal stem cells and endothelial progenitors into infarcted human myocardium. Catheterization and Cardiovascular Interventions 2005, 65:321-329.
37. Kiassov A.P., et al. Desmin expressing nonhematopoietic liver cells during rat liver development: an immunohistochemical and morphometric study. Differentiation 1995, 59:253-258.
38. Kordes C., et al. CD133+ hepatic stellate cells are progenitor cells. Biochemical and Biophysical Research Communications 2007, 352:410-417.
39. Le Blanc K., Ringden O. Immunobiology of human mesenchymal stem cells and future use in hematopoietic stem cell transplantation. Biology of Blood and Marrow Transplantation 2005, 11:321-334.
40. Le Blanc K., et al. HLA expression and immunologic properties of differentiated and undifferentiated mesenchymal stem cells. Experimental Hematology 2003, 31:890-896.
41. Legrier M.E., et al. Targeting protein translation in human non small cell lung cancer via combined MEK and mammalian target of rapamycin suppression. Cancer Research 2007, 67:11300-11308.
42. Liu F., et al. Changes in the expression of CD106, osteogenic genes, and transcription factors involved in the osteogenic differentiation of human bone marrow mesenchymal stem cells. Journal of Bone and Mineral Metabolism 2008, 26:312-320.
43. Mann J., Mann D.A. Transcriptional regulation of hepatic stellate cells. Advanced Drug Delivery Reviews 2009, 61:497-512.
44. McCuskey R.S. The hepatic microvascular system in health and its response to toxicants. The Anatomical Record (Hoboken) 2008, 291:661-671.
45. Mihara K., et al. Development and functional characterization of human bone marrow mesenchymal cells immortalized by enforced expression of telomerase. British Journal of Haematology 2003, 120:846-849.
46. Miyahara T., et al. Peroxisome proliferator-activated receptors and hepatic stellate cell activation. The Journal of Biological Chemistry 2000, 275:35715-35722.
47. Moreira R.K. Hepatic stellate cells and liver fibrosis. Archives of Pathology & Laboratory Medicine 2007, 131:1728-1734.
48. Niki T., et al. Class VI intermediate filament protein nestin is induced during activation of rat hepatic stellate cells. Hepatology 1999, 29:520-527.
49. Ozerdem U., et al. NG2 proteoglycan expression by pericytes in pathological microvasculature. Microvascular Research 2002, 63:129-134.
50. Reister S., et al. The epigenetic regulation of stem cell factors in hepatic stellate cells. Stem Cells Dev. 2011.
51. Russo F.P., et al. The bone marrow functionally contributes to liver fibrosis. Gastroenterology 2006, 130:1807-1821.
52. Schieker M., et al. Human mesenchymal stem cells at the single-cell level: simultaneous seven-colour immunofluorescence. Journal of Anatomy 2007, 210:592-599.
53. Senoo H., et al. Hepatic stellate cell (vitamin A-storing cell) and its relative past, present and future. Cell Biol Int. 2010, 34:1247-1272.
54. Silva W.A., et al. The profile of gene expression of human marrow mesenchymal stem cells. Stem Cells 2003, 21:661-669.
55. Stenderup K., et al. Aging is associated with decreased maximal life span and accelerated senescence of bone marrow stromal cells. Bone 2003, 33:919-926.
56. Strutz F., et al. Identification and characterization of a fibroblast marker: FSP1. The Journal of Cell Biology 1995, 130:393-405.
57. Suzuki K., et al. p75 Neurotrophin receptor is a marker for precursors of stellate cells and portal fibroblasts in mouse fetal liver. Gastroenterology 2008, 135(270-281):e3.
58. Tallquist M.D., et al. Additive effects of PDGF receptor beta signaling pathways in vascular smooth muscle cell development. PLoS Biology 2003, 1:E52.
59. Wake K. "Sternzellen" in the liver: perisinusoidal cells with special reference to storage of vitamin A. The American Journal of Anatomy 1971, 132:429-462.
60. Xu L., et al. Human hepatic stellate cell lines, LX-1 and LX-2: new tools for analysis of hepatic fibrosis. Gut 2005, 54:142-151.
61. Yavrom S., et al. Peroxisome proliferator-activated receptor gamma suppresses proximal alpha1(I) collagen promoter via inhibition of p300-facilitated NF-I binding to DNA in hepatic stellate cells. The Journal of Biological Chemistry 2005, 280:40650-40659.
62. Zimmermann A. Regulation of liver regeneration. Nephrology, Dialysis, Transplantation 2004, 19(Suppl 4):iv6-iv10.