Random mutagenesis of human estrogen receptor ligand binding domain identifies mutations that decrease sensitivity to estradiol and increase sensitivity to a diphenol indene-ol compound: Basis for a regulatable expression system

Journal of Steroid Biochemistry and Molecular Biology

Volumn 64, Issue 3-4, 1998, Pages 129-135

  Miller, Nicholas ^a   Whelan, James ^a  

^a GLAXOSMITHKLINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BETA GALACTOSIDASE; ESTRADIOL; ESTROGEN RECEPTOR; GR 132706X; UNCLASSIFIED DRUG;

ARTICLE; BINDING SITE; DNA LIBRARY; DRUG SENSITIVITY; GENE AMPLIFICATION; GENE CONSTRUCT; GENE EXPRESSION SYSTEM; IN VITRO STUDY; LIGAND BINDING; MUTAGENESIS; PHENOTYPE; POLYMERASE CHAIN REACTION; PROTEIN DOMAIN; SACCHAROMYCES CEREVISIAE; TECHNIQUE; YEAST;

AMINO ACID SEQUENCE; BINDING SITES; ESTRADIOL; GENE EXPRESSION REGULATION; GENES, REPORTER; HUMANS; INDENES; LIGANDS; MOLECULAR SEQUENCE DATA; MOLECULAR STRUCTURE; MUTAGENESIS; MUTATION; PHENOLS; PHENOTYPE; POLYMERASE CHAIN REACTION; RECEPTORS, ESTROGEN; SACCHAROMYCES CEREVISIAE; SUBSTRATE SPECIFICITY;

MAMMALIA; SACCHAROMYCES CEREVISIAE;

EID: 0031941755     PISSN: 09600760     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0960-0760(97)00154-4     Document Type: Article

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