Elucidating the interplay between DNA-condensing and free polycations in gene transfection through a mechanistic study of linear and branched PEI

Biomaterials

Volumn 32, Issue 33, 2011, Pages 8626-8634

  Dai, Zhuojun ^a,b   Gjetting, Torben ^a   Mattebjerg, Maria A ^a   Wu, Chi ^b   Andresen, Thomas L ^a  

^a TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

^b CHINESE UNIVERSITY OF HONG KONG   (Hong Kong)

Author keywords

Gene transfection; Non viral vectors; PEI; Polycations; Polyethylenimine; Polyplex

Indexed keywords

GENE TRANSFECTION; NONVIRAL VECTORS; POLYCATIONS; POLYETHYLENIMINES; POLYPLEXES;

CONDENSATION; DNA; EFFICIENCY; GENES; POLYETHERIMIDES;

MOLECULAR BIOLOGY;

LUCIFERASE; POLYCATION; POLYETHYLENEIMINE;

ARTICLE; CONTROLLED STUDY; DNA BINDING; DNA PROTEIN COMPLEX; ENDOCYTOSIS; ENZYME ACTIVITY; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; IN VITRO STUDY; INTERNALIZATION; MOLECULAR WEIGHT; PARTICLE SIZE; PRIMED IN SITU LABELING; PRIORITY JOURNAL; ZETA POTENTIAL;

DNA; HELA CELLS; HUMANS; POLYAMINES; POLYETHYLENEIMINE; TRANSFECTION;

EID: 80052963052     PISSN: 01429612     EISSN: 18785905     Source Type: Journal    
DOI: 10.1016/j.biomaterials.2011.07.044     Document Type: Article

References (39)

1. Pack D.W., Hoffman A.S., Pun S., Stayton P.S. Design and development of polymers for gene delivery. Nat Rev Drug Discov 2005, 4:581-593.
2. Mintzer M.A., Simanek E.E. Nonviral vectors for gene delivery. Chem Rev 2009, 109:259-302.
3. Boussif O., Lezoualch F., Zanta M.A., Mergny M.D., Scherman D., Demeneix B., et al. A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: polyethylenimine. Proc Natl Acad Sci U S A 1995, 92:7297-7301.
4. Lungwitz U., Breunig M., Blunk T., Gopferich A. Polyethylenimine-based non-viral gene delivery systems. Eur J Pharm Biopharm 2005, 60:247-266.
5. Neu M., Fischer D., Kissel T. Recent advances in rational gene transfer vector design based on poly(ethylene imine) and its derivatives. J Gene Med 2005, 7:992-1009.
6. Parhamifar L., Larsen A.K., Hunter A.C., Andresen T.L., Moghimi S.M. Polycation cytotoxicity: a delicate matter for nucleic acid therapy-focus on polyethylenimine. Soft Matter 2010, 6:4001-4009.
7. Dunlap D.D., Maggi A., Soria M.R., Monaco L. Nanoscopic structure of DNA condensed for gene delivery. Nucleic Acids Res 1997, 25:3095-3101.
8. Boussif O., Zanta M.A., Behr J.P. Optimized galenics improve in vitro gene transfer with cationic molecules up to 1000-fold. Gene Ther 1996, 3:1074-1080.
9. Morille M., Passirani C., Vonarbourg A., Clavreul A., Benoit J.P. Progress in developing cationic vectors for non-viral systemic gene therapy against cancer. Biomaterials 2008, 29:3477-3496.
10. Ogris M., Steinlein P., Kursa M., Mechtler K., Kircheis R., Wagner E. The size of DNA/transferrin-PEI complexes is an important factor for gene expression in cultured cells. Gene Ther 1998, 5:1425-1433.
11. Kircheis R., Kichler A., Wallner G., Kursa M., Ogris M., Felzmann T., et al. Coupling of cell-binding ligands to polyethylenimine for targeted gene delivery. Gene Ther 1997, 4:409-418.
12. Fischer D., Bieber T., Li Y., Elsässer H.P., Kissel T. A novel non-viral vector for DNA delivery based on low molecular weight, branched polyethylenimine: effect of molecular weight on transfection efficiency and cytotoxicity. Pharm Res 1999, 16:1273-1279.
13. Itaka K., Harada A., Yamasaki Y., Nakamura K., Kawaguchi H., Kataoka K. In situ single cell observation by fluorescence resonance energy transfer reveals fast intra-cytoplasmic delivery and easy release of plasmid DNA complexed with linear polyethylenimine. J Gene Med 2004, 6:76-84.
14. Wightman L., Kircheis R., Rossler V., Carotta S., Ruzicka R., Kursa M., et al. Different behavior of branched and linear polyethylenimine for gene delivery in vitro and in vivo. J Gene Med 2001, 3:362-372.
15. Godbey W.T., Wu K.K., Mikos A.G. Size matters: molecular weight affects the efficiency of poly(ethylenimine) as a gene delivery vehicle. J Biomed Mater Res 1999, 45:268-275.
16. Wagner E., Cotten M., Foisner R., Birnstiel M.L. Transferrin-polycation-DNA complexes: the effect of polycations on the structure of the complex and DNA delivery to cells. Proc Natl Acad Sci U S A 1991, 88:4255-4259.
17. Kunath K., von Harpe A., Fischer D., Peterson H., Bickel U., Voigt K., et al. Low-molecular-weight polyethylenimine as a non-viral vector for DNA delivery: comparison of physicochemical properties, transfection efficiency and in vivo distribution with high-molecular-weight polyethylenimine. J Control Release 2003, 89:113-125.
18. Godbey W.T., Wu K.K., Mikos A.G. Tracking the intracellular path of poly(ethylenimine)/DNA complexes for gene delivery. Proc Natl Acad Sci U S A 1999, 96:5177-5181.
19. Pollard H., Remy J.S., Loussouarn G., Demolombe S., Behr J.P., Escande D. Polyethylenimine but not cationic lipids promotes transgene delivery to the nucleus in mammalian cells. J Biol Chem 1998, 273:7507-7511.
20. Ogris M., Brunner S., Schuller S., Kircheis R., Wagner E. PEGylated DNA/transferrin-PEI complexes: reduced interaction with blood components, extended circulation in blood and potential for systemic gene delivery. Gene Ther 1999, 6:595-605.
21. Kircheis R., Schuller S., Brunner S., Ogris M., Heider K.H., Zauner W., et al. Polycation-based DNA complexes for tumor-targeted gene delivery in vivo. J Gene Med 1999, 1:111-120.
22. Erbacher P., Bettinger T., Belguise-Valladier P., Zou S., Coll J.L., Behr J.P., et al. Transfection and physical properties of various saccharide, poly(ethylene glycol), and antibody-derivatized polyethylenimines (PEI). J Gene Med 1999, 1:210-222.
23. Boeckle S., von Gersdorff K., van der Piepen S., Culmsee C., Wagner E., Ogris M. Purification of polyethylenimine polyplexes highlights the role of free polycations in gene transfer. J Gene Med 2004, 6:1102-1111.
24. Yue Y., Jin F., Deng R., Cai J., Dai Z., Lin M.C., et al. Revisit complexation between DNA and polyethylenimine - effect of length of free polycationic chains on gene transfection. J Control Release 2011, 152:143-151.
25. Behr J.P. The proton sponge: a trick to enter cells the viruses did not exploit. Chimia 1997, 51:34-36.
26. Sonawane N.D., Szoka F.C., Verkman A.S. Chloride accumulation and swelling in endosomes enhances DNA transfer by polyamine-DNA polyplexes. J Biol Chem 2003, 278:44826-44831.
27. Won Y., Sharma R., Konieczny S.F. Missing pieces in understanding the intracellular trafficking of polycation/DNA complexes. J Control Release 2009, 139:88-93.
28. Kabanov A.V., Kabanov V.A. DNA complexes with polycations for the delivery of genetic material into cells. Bioconjug Chem 1995, 6:7-20.
29. Kircheis R., Blessing T., Brunner S., Wightman L., Wagner E. Tumor targeting with surface-shielded ligand-polycation DNA complexes. J Control Release 2001, 72:165-170.
30. Gabrielson N.P., Pack D.W. Efficient polyethylenimine-mediated gene delivery proceeds via a caveolar pathway in HeLa cells. J Control Release 2009, 136:54-61.
31. Thomas M., Lu J.J., Ge Q., Zhang C., Chen J., Klibanov A.M. Full deacylation of polyethylenimine dramatically boosts its gene delivery efficiency and specificity to mouse lung. Proc Natl Acad Sci U S A 2005, 102:5679-5684.
32. Wiseman J.W., Goddard C.A., McLelland D., Colledge W.H. A comparison of linear and branched polyethylenimine (PEI) with DCChol/DOPE liposomes for gene delivery to epithelial cells in vitro and in vivo. Gene Ther 2003, 10:1654-1662.
33. Zhang C., Gao S., Jiang W., Lin S., Du F., Li Z., et al. Targeted minicircle DNA delivery using folate-poly(ethylene glycol)-polyethylenimine as non-viral carrier. Biomaterials 2010, 31:6075-6086.
34. Furgeson D.Y., Yockman J.W., Janat M.M., Kim S.W. Tumor efficacy and biodistribution of linear polyethylenimin-cholesterol/DNA complexes. Mol Ther 2004, 9:837-845.
35. Deng R., Yue Y., Jin F., Chen Y.C., Kung H.F., Lin M.C., et al. Revisit the complexation of PEI and DNA - how to make low cytotoxic and highly efficient PEI gene transfection non-viral vectors with a controllable chain length and structure?. J Control Release 2009, 140:40-46.
36. Remy J.S., Abdallah B., Zanta M.A., Boussif O., Behr J.P., Demeneix B. Gene transfer with lipospermines and polyethylenimines. Adv Drug Deliv Rev 1998, 30:85-95.
37. Jeong J.H., Song S.H., Lim D.W., Lee H., Park T.G. DNA transfection using linear poly(ethylenimine) prepared by controlled acid hydrolysis of poly(2-ethyl-2-oxazoline). J Control Release 2001, 73:391-399.
38. Kircheis R., Wightman L., Wagner E. Design and gene delivery activity of modified polyethylenimines. Adv Drug Deliv Rev 2001, 53:341-358.
39. Bieber T., Elsässer H.P. Preparation of a low molecular weight polyethylenimine for efficient cell transfection. Biotechniques 2001, 30:74-81.