Expression of an optimized Argopecten purpuratus antimicrobial peptide in E. coli and evaluation of the purified recombinant protein by in vitro challenges against important plant fungi

Peptides

Volumn 32, Issue 9, 2011, Pages 1909-1916

  Tapia, Eduardo ^a,c   Montes, Christian ^c   Rebufel, Patricia ^c   Paradela, Alberto ^d   Prieto, Humberto ^c   Arenas, Gloria ^a,b  

^a DEPARTAMENTO DE FÍSICA   (Chile)

^b PONTIFICIA UNIVERSIDAD CATÓLICA DE VALPARAÍSO   (Chile)

^c INSTITUTO DE INVESTIGACIONES AGROPECUARIAS   (Chile)

^d CENTRO NACIONAL DE BIOTECNOLOGÍA   (Spain)

Author keywords

Antifungal peptide; cDNA design; Chimaeric gene sequence; Fungal plant pathogen; Recombinant E. coli expression

Indexed keywords

ANTIFUNGAL AGENT; POLYPEPTIDE ANTIBIOTIC AGENT; PROTEIN APS; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

ALTERNARIA; ANTIFUNGAL ACTIVITY; ARGOPECTEN PURPURATUS; ARTICLE; BOTRYTIS CINEREA; CLONING; DRUG PURIFICATION; DRUG SYNTHESIS; ESCHERICHIA COLI; FUSARIUM OXYSPORUM; GROWTH INHIBITION; HYPOCREA LIXII; IN VITRO STUDY; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; SCALLOP;

ALTERNARIA; AMINO ACID SEQUENCE; ANIMALS; ANTIFUNGAL AGENTS; BASE SEQUENCE; BOTRYTIS; CLONING, MOLECULAR; COLONY COUNT, MICROBIAL; ESCHERICHIA COLI; FUSARIUM; GENES, SYNTHETIC; GENETIC VECTORS; MICROBIAL SENSITIVITY TESTS; MOLECULAR SEQUENCE DATA; PECTINIDAE; PEPTIDES; RECOMBINANT PROTEINS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; TRICHODERMA;

ALTERNARIA; ARGOPECTEN PURPURATUS; BOTRYOTINIA FUCKELIANA; FUNGI; FUSARIUM OXYSPORUM; HYPOCREA LIXII; SOLANUM TUBEROSUM;

EID: 80052718442     PISSN: 01969781     EISSN: 18735169     Source Type: Journal    
DOI: 10.1016/j.peptides.2011.07.007     Document Type: Article

References (39)

1. D. Andreu, J. Ubach, A. Boman, B. Wahlin, D. Wade, and R. Merrifield Shortened cecropin A-mellitin hybrids significant size reduction retains potent antibiotic activity FEBS Lett 296 1992 190 194
2. G. Arenas, F. Guzmán, C. Cárdenas, L. Mercado, and S. Marshall A novel antifungal peptide designed from the primary structure of a natural antimicrobial peptide purified from Agropecten purpuratus hemocytes Peptides 30 2009 1405 1411
3. A. Bansemir, M. Blume, S. Schroder, and U. Lindequist Screening of cultivated seaweeds for antibacterial activity against fish pathogenic bacteria Aquaculture 252 2006 79 84 (Pubitemid 43188764)
4. M. Bradford A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal Biochem 72 1976 248 254
5. M. Brahmachary, S. Krishnan, J. Koh, A. Khan, S. Seah, and T. Tan ANTIMIC: a database of antimicrobial sequences Nucleic Acids Res 32 2004 586 589
6. W. Broekaert, B. Cammue, M. De Bolle, K. Thevissen, G. De Samblanx, and R. Osborn Antimicrobial peptides from plants Crit Rev Plant Sci 16 1997 297 323 (Pubitemid 127468279)
7. P. Bulet, J. Dimarcq, C. Hetru, M. Lagueux, M. Charlet, and G. Hegy Antimicrobial peptides: from invertebrate to vertebrate Immunol Rev 198 2004 169 184
8. M. Charlet, S. Chernysh, H. Philippe, C. Hetru, J. Hoffmann, and P. Bulet Innate immunity: isolation of several cysteine-rich antimicrobial peptides from the blood of a mollusc Mytilus edulis J Biol Chem 271 1996 21808 21813 (Pubitemid 26303799)
9. M. Costa, S. Dios, J. Alonso-Gutierrez, A. Romero, B. Novoa, and A. Figueras Myticin C variability in mussel: ancient defense mechanism or self/non self discrimination? Dev Comp Immunol 33 2009 162 170
10. M. Dathe, and T. Wieprecht Structural features of helical antimicrobial peptides: their potential to modulate activity on model membranes and biological cells Biochim Biophys Acta 1462 1999 71 87
11. A. De Lucca, and T. Walsh Antifungal peptides: novel therapeutic compounds against emerging pathogens Antimicrob Agents Chemother 43 1999 1 11 (Pubitemid 29069190)
12. M. De Zoysa, Nikapitiya Ch, I. Whang, J.S. Lee, J. Lee, and Abhisin A potential antimicrobial peptide derived from histone H2A of disk abalone (Haliotis discus discus) Fish Shellfish Immunol 27 2009 639 646
13. C. Fjell, R. Hancock, and A. Cherkasov AMPer: a database and a automated discovery tool for antimicrobial peptides Bioinformatics 23 2007 1148 1155 (Pubitemid 47050567)
14. M. Fuhrmann, A. Hausherr, L. Ferbitz, T. Schödl, M. Heitzer, and P. Hegemann Monitoring dynamic expression of nuclear genes in Chlamydomonas reinhardtii by using a synthetic luciferase reporter gene Plant Mol Biol 55 2004 869 881 (Pubitemid 40318812)
15. A. Gao, S. Hakimi, C. Mittanck, Y. Wu, B. Woerner, and D. Stark Fungal pathogen protection in potato by expression of a plant defensin peptide Nat Biotechnol 18 2000 1307 1310
16. C. Gestal, M. Costa, A. Figueras, and B. Novoa Analysis of differentially expressed genes in response to bacterial stimulation in hemocytes of the carpet-shell clam Ruditapes decussatus: identification of new antimicrobial peptides Gene 406 2007 134 143 (Pubitemid 350216300)
17. Y. Gueguen, A. Herpin, A. Aumelas, J. Garnier, J. Fievet, and J. Escoubas Characterization of a defensin from the oyster Crassostrea gigas. Recombinant production, folding, solution structure, antimicrobial activities, and gene expression J Biol Chem 281 2006 313 323 (Pubitemid 43671191)
18. F. Hu, T. Ke, X. Li, P. Mao, X. Jin, and F. Hui Purification of an antimicrobial peptide by fusion with elastin-like polypeptides in Escherichia coli Appl Biochem Biotechnol 160 2009 2377 2387
19. H. Jenssen, P. Hamill, and R. Hancock Peptide antimicrobial agents Clin Microbiol Rev 19 2006 491 511 (Pubitemid 44137212)
20. D. Lee, S. Shin, K. Hahm, and Structure fungicidal activity of a synthetic antimicrobial peptide, P18, and its truncated peptides Biotechnol Lett 26 2004 337 341 (Pubitemid 38272267)
21. R. Lehrer, and T. Ganz Defensin of vertebrate animals Curr Opin Immunol 14 2002 96 102
22. X. Li, Z. Dai, S. Zhang, and L. Zhang Studies on the changes of CM4 gene structure and the expression products in E coli Acta Biochim Biophys Sinica 15 1999 387 391
23. C. Li, L. Song, J. Zhao, L. Zhu, H. Zou, and H. Zhang Preliminary study on a potential antibacterial peptide derived from histone H2A in hemocytes of scallop Chlamys farreri Fish Shellfish Immunol 22 2007 663 672 (Pubitemid 46386453)
24. F. Lustig, J. Hoebeke, G. Ostergren-Lunden, F. Velge-Roussel, G. Bondjers, and U. Olsson Alternative splicing determines the binding of platelet-derived growth factor (PDGF-AA) to glycosaminoglycans Biochemistry 35 1996 12077 12085 (Pubitemid 26313679)
25. S. Makrides Strategies for achieving high level expression of genes in Escherichia coli Microbiol Rev 60 1996 512 538 (Pubitemid 26303477)
26. S. Marshall, and G. Arenas Antimicrobial peptides: a natural alternative to chemical antibiotics and a potential for applied biotechnology Electron J Biotechnol 6 2003 271 284
27. G. Mitta, F. Hubert, T. Noel, P. Roch, and Myticin a novel cysteine-rich antimicrobial peptide isolated from haemocytes and plasma of the mussel Mytilus galloprovincialis Eur J Biochem 265 1999 71 78 (Pubitemid 29466013)
28. H. Oh, M. Hedberg, D. Wade, and C. Edlund Activities of synthetic hybrid peptides against anaerobic bacteria: aspects methodology and stability Antimicrob Agents Chemother 44 2000 68 72 (Pubitemid 30010674)
29. A. Padhi, and B. Verghese Molecular diversity and evolution of mycitin-C antimicrobial peptide variants in the Mediterranean mussel, Mytilus galloprovincialis Peptides 7 2008 1094 1101
30. J. Powers, and R. Hancock The relationship between peptide structure and antibacterial activity Peptides 24 2003 1681 1691 (Pubitemid 38198075)
31. J. Sambrook, E. Fritsch, and T. Maniatis Molecular cloning: a laboratory manual 2nd ed. 1989 Cold Spring Harbor Lab Press New York
32. S. Soltani, K. Keymanesh, and S. Sardari In silico analysis of antifungal peptides: determining the lead template sequence of potent antifungal peptides Expert Opin Drug Discov 2 2007 1 11
33. J. Tincu, and S. Taylor Antimicrobial peptides from marine invertebrates Antimicrob Agents Chemother 48 2004 3645 3654 (Pubitemid 39304585)
34. L. Wang, B. Yu, G. Han, J. He, D. Chen, and Design expression and characterization of recombinant hybrid peptide Attacin-Thanatin in Escherichia coli Mol Biol Rep 37 2010 3495 3501
35. Z. Wang, and G. Wang APD: the antimicrobial peptide database Nucleic Acids Res 32 2004 590 592
36. G. Wu, X. Li, X. Ding, Z. Wen, and L. Shen High-Level expression and novel purification strategy of recombinant Thanatin analog in Escherichia coli Curr Microbiol 57 2008 95 101
37. R.K. Wright Urochordates vol. 2 1981 Academic Press, Ltd. London, England
38. R. Wright, M. Yeaman, and N. Yount Mechanisms of antimicrobial peptide action and resistance Pharmacol Rev 55 2003 27 55 (Pubitemid 36268398)
39. M. Zasloff Antimicrobial peptides of multicellular organisms Nature 415 2002 389 395 (Pubitemid 34100944)