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Volumn 160, Issue 8, 2010, Pages 2377-2387

Expression and purification of an antimicrobial peptide by fusion with elastin-like polypeptides in escherichia coli

Author keywords

Antimicrobial polypides; Elastin like polypeptide; Fusion protein; Halocidin; Soluble expression

Indexed keywords

ANTI-MICROBIAL ACTIVITY; ANTIMICROBIAL PEPTIDE; CARRIER MOLECULES; CULTURE MEDIUM; ELASTIN-LIKE POLYPEPTIDES; FUSION PARTNERS; FUSION PROTEINS; HOST CELLS; INVERSE TRANSITION; LOW PURITY; MICROCOCCUS LUTEUS; PICHIA PASTORIS; RECOMBINANT PROTEIN EXPRESSION; SCALING-UP; SOLUBLE EXPRESSION;

EID: 77949912931     PISSN: 02732289     EISSN: None     Source Type: Journal    
DOI: 10.1007/s12010-009-8850-2     Document Type: Article
Times cited : (26)

References (33)
  • 1
    • 0347755460 scopus 로고    scopus 로고
    • 10.1093/nar/gkh025 1:CAS:528:DC%2BD3sXhtVSrur%2FI
    • Z Wang G Wang 2004 Nucleic Acids Research 32 D590 D592 10.1093/nar/gkh025 1:CAS:528:DC%2BD3sXhtVSrur%2FI
    • (2004) Nucleic Acids Research , vol.32
    • Wang, Z.1    Wang, G.2
  • 3
    • 30044452344 scopus 로고    scopus 로고
    • Cationic host defense (antimicrobial) peptides
    • DOI 10.1016/j.coi.2005.11.004, PII S0952791505001998, Innate Immunity/Antigen Processing and Recognition
    • KL Brown REW Hancock 2006 Current Opinion in Immunology 18 24 30 10.1016/j.coi.2005.11.004 1:CAS:528:DC%2BD28XhvFOhtQ%3D%3D (Pubitemid 43049644)
    • (2006) Current Opinion in Immunology , vol.18 , Issue.1 , pp. 24-30
    • Brown, K.L.1    Hancock, R.E.W.2
  • 4
    • 0037165196 scopus 로고    scopus 로고
    • Antimicrobial peptides of multicellular organisms
    • DOI 10.1038/415389a
    • M Zasloff 2002 Nature 415 389 395 10.1038/415389a 1:CAS:528: DC%2BD38XhtVCgsLs%3D (Pubitemid 34100944)
    • (2002) Nature , vol.415 , Issue.6870 , pp. 389-395
    • Zasloff, M.1
  • 6
    • 3543116141 scopus 로고    scopus 로고
    • High-level expression and purification of a recombinant hBD-1 fused to LMM protein in Escherichia coli
    • DOI 10.1016/j.pep.2004.04.024, PII S1046592804001226
    • I Cipakova E Hostinova J Gasperik V Velebny 2004 Protein Expression and Purification 37 207 212 10.1016/j.pep.2004.04.024 1:CAS:528:DC%2BD2cXmt1ClsL4%3D (Pubitemid 39027262)
    • (2004) Protein Expression and Purification , vol.37 , Issue.1 , pp. 207-212
    • Cipakova, I.1    Hostinova, E.2    Gasperik, J.3    Velebny, V.4
  • 7
    • 25144438913 scopus 로고    scopus 로고
    • Facilitation of expression and purification of an antimicrobial peptide by fusion with baculoviral polyhedrin in Escherichia coli
    • DOI 10.1128/AEM.71.9.5038-5043.2005
    • W Quande KS Young SH Jeong SJ Woong LI Hee CJ Hyung 2005 Applied and Environmental Microbiology 71 5038 5043 10.1128/AEM.71.9.5038-5043.2005 (Pubitemid 41338228)
    • (2005) Applied and Environmental Microbiology , vol.71 , Issue.9 , pp. 5038-5043
    • Wei, Q.1    Kim, Y.S.2    Seo, J.H.3    Jang, W.S.4    Lee, I.H.5    Cha, H.J.6
  • 11
    • 0029664501 scopus 로고    scopus 로고
    • Production in Escherichia coli of moricin, a novel type antibacterial peptide from the silkworm, Bombyx mori
    • DOI 10.1006/bbrc.1996.0461
    • S Hara M Yamakawa 1996 Biochemical and Biophysical Research Communications 22 664 669 10.1006/bbrc.1996.0461 (Pubitemid 26199274)
    • (1996) Biochemical and Biophysical Research Communications , vol.220 , Issue.3 , pp. 664-669
    • Hara, S.1    Yamakawa, M.2
  • 12
    • 0347597093 scopus 로고    scopus 로고
    • Expressing an antibacterial protein in bacteria for raising antibodies
    • DOI 10.1016/j.pep.2003.08.026
    • PJ Barrell OW Liew AJ Conner 2004 Protein Expression and Purification 33 153 159 10.1016/j.pep.2003.08.026 1:CAS:528:DC%2BD3sXpt1Wju7o%3D (Pubitemid 38069823)
    • (2004) Protein Expression and Purification , vol.33 , Issue.1 , pp. 153-159
    • Barrell, P.J.1    Liew, O.W.2    Conner, A.J.3
  • 13
    • 33646726320 scopus 로고    scopus 로고
    • Cloning, expression, isotope labeling, and purification of human antimicrobial peptide LL-37 in Escherichia coli for NMR studies
    • DOI 10.1016/j.pep.2005.10.022, PII S1046592805003803
    • YF Li X Li GS Wang 2006 Protein Expression and Purification 47 498 505 10.1016/j.pep.2005.10.022 1:CAS:528:DC%2BD28XltVKit78%3D (Pubitemid 43744452)
    • (2006) Protein Expression and Purification , vol.47 , Issue.2 , pp. 498-505
    • Li, Y.1    Li, X.2    Wang, G.3
  • 14
    • 0023953406 scopus 로고
    • 10.1007/BF01025411 1:CAS:528:DyaL1cXht1KksbY%3D
    • DW Urry 1988 Journal of Protein Chemistry 7 1 34 10.1007/BF01025411 1:CAS:528:DyaL1cXht1KksbY%3D
    • (1988) Journal of Protein Chemistry , vol.7 , pp. 1-34
    • Urry, D.W.1
  • 15
    • 0026468684 scopus 로고
    • 10.1016/0079-6107(92)90003-O 1:CAS:528:DyaK38Xhs1ygu74%3D
    • DW Urry 1992 Progress in Biophysics and Molecular Biology 57 23 57 10.1016/0079-6107(92)90003-O 1:CAS:528:DyaK38Xhs1ygu74%3D
    • (1992) Progress in Biophysics and Molecular Biology , vol.57 , pp. 23-57
    • Urry, D.W.1
  • 16
    • 0038388786 scopus 로고    scopus 로고
    • 1:CAS:528:DyaK2sXns1Glt78%3D
    • DW Urry 1997 Journal of Physical Chemistry 101 11007 11028 1:CAS:528:DyaK2sXns1Glt78%3D
    • (1997) Journal of Physical Chemistry , vol.101 , pp. 11007-11028
    • Urry, D.W.1
  • 18
    • 9344234999 scopus 로고    scopus 로고
    • Expression and purification of recombinant proteins from Escherichia coli: Comparison of an elastin-like polypeptide fusion with an oligohistidine fusion
    • DOI 10.1110/ps.04931604
    • KT Carlson L Liu K Bumjoon A Chilkoti 2004 Protein Science 13 3274 3284 10.1110/ps.04931604 (Pubitemid 39557797)
    • (2004) Protein Science , vol.13 , Issue.12 , pp. 3274-3284
    • Trabbic-Carlson, K.1    Liu, L.2    Kim, B.3    Chilkoti, A.4
  • 19
    • 0032739304 scopus 로고    scopus 로고
    • Purification of recombinant proteins by fusion with thermally-responsive polypeptides
    • DOI 10.1038/15100
    • DE Meyer A Chilkoti 1999 Nature Biotechnology 17 1112 1115 10.1038/15100 1:CAS:528:DyaK1MXnt1Sqtr0%3D (Pubitemid 29533553)
    • (1999) Nature Biotechnology , vol.17 , Issue.11 , pp. 1112-1115
    • Meyer, D.E.1    Chilkoti, A.2
  • 20
    • 0034878076 scopus 로고    scopus 로고
    • Protein purification by fusion with an environmentally responsive elastin-like polypeptide: Effect of polypeptide length on the purification of thioredoxin
    • DOI 10.1021/bp010049o
    • DE Meyer KT Carlson A Chilkoti 2001 Biotechnology Progress 17 4 720 728 10.1021/bp010049o 1:CAS:528:DC%2BD3MXksFelsrw%3D (Pubitemid 32748063)
    • (2001) Biotechnology Progress , vol.17 , Issue.4 , pp. 720-728
    • Meyer, D.E.1    Trabbic-Carlson, K.2    Chilkoti, A.3
  • 21
    • 0037235978 scopus 로고    scopus 로고
    • The molecular basis of the temperature- and PH-induced conformational transitions in elastin-based peptides
    • DOI 10.1002/bip.10204
    • B Li V Daggett 2003 Biopolymers 68 121 129 10.1002/bip.10204 1:CAS:528:DC%2BD3sXitlyqsA%3D%3D (Pubitemid 36098312)
    • (2003) Biopolymers , vol.68 , Issue.1 , pp. 121-129
    • Li, B.1    Daggett, V.2
  • 22
    • 0037134853 scopus 로고    scopus 로고
    • Halocidin: A new antimicrobial peptide from hemocytes of the solitary tunicate, Halocynthia aurantium
    • DOI 10.1016/S0014-5793(02)02827-2, PII S0014579302028272
    • WS Jang KN Kim YS Lee MH Nam IH Lee 2002 FEBS Letters 521 81 86 10.1016/S0014-5793(02)02827-2 1:CAS:528:DC%2BD38XksVaiu7s%3D (Pubitemid 34628463)
    • (2002) FEBS Letters , vol.521 , Issue.1-3 , pp. 81-86
    • Jang, W.S.1    Kim, K.N.2    Lee, Y.S.3    Nam, M.H.4    Lee, I.H.5
  • 23
    • 0035038213 scopus 로고    scopus 로고
    • Optimization of the hydroxylamine cleavage of an expressed fusion protein to produce a recombinant antimicrobial peptide
    • DOI 10.1023/A:1010358131124
    • HB Park SH Pyo SS Hong JH Kim 2001 Biotechnology Letters 23 637 641 10.1023/A:1010358131124 1:CAS:528:DC%2BD3MXjslWju7k%3D (Pubitemid 32385668)
    • (2001) Biotechnology Letters , vol.23 , Issue.8 , pp. 637-641
    • Park, H.-B.1    Pyo, S.-H.2    Hong, S.-S.3    Kim, J.-H.4
  • 24
    • 34248679167 scopus 로고    scopus 로고
    • 10.1038/nprot.2006.4
    • S Hermann 2006 Nat Protocol 1 16 22 10.1038/nprot.2006.4
    • (2006) Nat Protocol , vol.1 , pp. 16-22
    • Hermann, S.1
  • 28
    • 34547856210 scopus 로고    scopus 로고
    • High-level expression of the recombinant hybrid peptide cecropinA(1-8)-magainin2(1-12) with an ubiquitin fusion partner in Escherichia coli
    • DOI 10.1016/j.pep.2007.04.018, PII S1046592807001192
    • X Xu F Jin X Yu S Ren J Hu W Zhang 2007 Protein Expression and Purification 55 1 175 182 10.1016/j.pep.2007.04.018 1:CAS:528: DC%2BD2sXpt1antbY%3D (Pubitemid 47260742)
    • (2007) Protein Expression and Purification , vol.55 , Issue.1 , pp. 175-182
    • Xu, X.1    Jin, F.2    Yu, X.3    Ren, S.4    Hu, J.5    Zhang, W.6
  • 29
    • 33745026919 scopus 로고    scopus 로고
    • Ultra-high expression of a thermally responsive recombinant fusion protein in E. coli
    • DOI 10.1021/bp0503742
    • DC Chow MR Dreher CK Trabbic A Chilkoti 2006 Biotechnology Progress 22 3 638 646 10.1021/bp0503742 1:CAS:528:DC%2BD28XivVChtbk%3D (Pubitemid 43877431)
    • (2006) Biotechnology Progress , vol.22 , Issue.3 , pp. 638-646
    • Chow, D.C.1    Dreher, M.R.2    Trabbic-Carlson, K.3    Chilkoti, A.4
  • 30
    • 0017637022 scopus 로고
    • 10.1016/0076-6879(77)47016-2 1:CAS:528:DyaE1cXltFShurk%3D
    • P Bornstein G Balian 1977 Methods in Enzymology 47 132 145 10.1016/0076-6879(77)47016-2 1:CAS:528:DyaE1cXltFShurk%3D
    • (1977) Methods in Enzymology , vol.47 , pp. 132-145
    • Bornstein, P.1    Balian, G.2
  • 31
    • 0035038213 scopus 로고    scopus 로고
    • Optimization of the hydroxylamine cleavage of an expressed fusion protein to produce a recombinant antimicrobial peptide
    • DOI 10.1023/A:1010358131124
    • B Heung HP Sang SH Seung HK Jin 2001 Biotechnology Letters 23 637 641 10.1023/A:1010358131124 (Pubitemid 32385668)
    • (2001) Biotechnology Letters , vol.23 , Issue.8 , pp. 637-641
    • Park, H.-B.1    Pyo, S.-H.2    Hong, S.-S.3    Kim, J.-H.4
  • 32
    • 0043270583 scopus 로고    scopus 로고
    • Biological activities of synthetic analogs of halocidin, an antimicrobial peptide from the tunicate Halocynthia aurantium
    • DOI 10.1128/AAC.47.8.2481-2486.2003
    • WS Jang CH Kim KN Kim SY Park JH Lee SM Son, et al. 2003 Antimicrobial Agents and Chemotherapy 47 8 2481 2486 10.1128/AAC.47.8.2481-2486.2003 1:CAS:528:DC%2BD3sXlvFCmu7o%3D (Pubitemid 36919448)
    • (2003) Antimicrobial Agents and Chemotherapy , vol.47 , Issue.8 , pp. 2481-2486
    • Jang, W.S.1    Kim, C.H.2    Kim, K.N.3    Park, S.Y.4    Lee, J.H.5    Son, S.M.6    Lee, I.H.7


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