Purification and characterization of highly thermostable amylopullulanase from a thermophilic, anaerobic bacterium Clostridium thermosulfurogenes SVM17

Malaysian Journal of Microbiology

Volumn 7, Issue 2, 2011, Pages 97-106

  Mrudula, Soma ^a   Reddy, Gopal ^b   Seenayya, Gunda ^c  

^a MGR College   (India)

^b OSMANIA UNIVERSITY   (India)

^c R and D Issar Pharmaceuticals Pvt Ltd   (India)

Author keywords

Amylase; Characterization; Clostridium thermosulfurogenes SVM17; Pullulanase; Purification

Indexed keywords

EID: 80051955647     PISSN: 22317538     EISSN: 18238262     Source Type: Journal    
DOI: None     Document Type: Article

References (51)

1. Antranikian, G., Herzberg, C. and Gottschalk. G. (1987). Production of thermostable α-amylase, pullulanase, and α-glucosidase in continuous culture by a new Clostridium isolate, Applied and Environonmental Microbiology 53, 1668-1673.
2. Ara, K., Igarashi, K., Shigihara, H., Sawada, K., Kobayashi, T. and Ito, S. (1996). Separation of functional domains for the α-1,4 and α-1,6 hydrolytic activities of Bacillus amylopullulanase by limited proteolysis with papain. Bioscience, Biotechnology and Biochemistry 60, 634-639.
3. Ara, K., Igarashi, K., Saeki, K., Kawai, S. and Ito, S. (1992). Purification and some properties of an alkaline pullulanase from alkaliphilic Bacillus sp. KSM-1876. Bioscience, Biotechnology and Biochemistry 56, 62-65.
4. Ara, K., Igarashi, K., Saeki, K. and Ito, S. (1995). An alkaline amylopullulanase from alkaliphilic Bacillus sp. KSM-1378: Kinetic evidence for two independent active sites for the α-1,4 and α-1,6 hydrolytic reactions. Bioscience, Biotechnology and Biochemistry 59, 662-666.
5. Arikan, B. (2008). Highly thermostable, thermophilic, alkaline, SDS and chelator resistant amylase from a thermophilic Bacillus sp. Isolate A3-15. Bioresource Technology 99, 3071-3076.
6. Bernhardsdotter, E. C. M. J., Joseph, D. N. G., Garriott, O. K. and Pusey, M. L. (2005). Enzymatic properties of an alkaline chelator-resistant α-amylase from an alkaliphilic Bacillus sp. isolate L1711. Process Biochemistry 40, 2401-2408.
7. Blum, I., Beier, H. and Gross, H. J. (1987). Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels. Electrophoresis 8, 93-99.
8. Brown, S. H. and Kelly, R. M. (1993). Characterization of amylolytic enzymes, having both α-1,4 and α-1,6 hydrolytic activity, from the thermophilic archea Pyrococcus furiosus and Thermococcus litoralis. Applied and Environmental Microbiology 59, 2614-2621.
9. Deweer, P., and Amory, A. (2000). Acid-stable and thermostable enzymes derived from Sulfolobus species. USP No.6,100,073.
10. Dong, G., Vieille, C. and Zeikus, J. G. (1997). Cloning, sequencing and expression of the gene encoding amylopullulanase from Pyrococcus furiosus and biochemical characterization of the recombinant enzyme. Applied and Environmental Microbiology 63, 3577-3584.
11. Duffner, F., Bertoldo, C., Andersen, J. T., Wagner, K. and Antranikian, G. (2000). A new thermoactive pullulanase from Desulfurococcus mucosus: Cloning, sequencing, purification and characterization of the recombinent enzyme after expression in Bacillus subtilis. Journal of Bacteriology 182, 6331-6338.
12. Fogarty, W. M. and Kelly, C. T. (1994). Extracellular maltotetrose forming amylase of Pseudomonas sp. IMD 353. Biotechnology Letters 16, 473-478.
13. Ganghofner, D., Kellermann, J., Staudenbauer, L.W. and Bronnenmeier, K. (1998). Purification and properties of an amylopullulanase, a glucoamylase, and an α-glucosidase in the amylolytic enzyme system of Thermoanaerobacterium thermosaccharolyticum. Bioscience Biotechnology and Biochemistry 62, 302-308.
14. Gantelet, H. and Duchiron, F. (1998). Purification and properties of a thermoactive and thermostable pullulanase from Thermococcus hydrothermalis. a hyperthermophilic archaeon isolated from a deep-sea hydrothermal vent. Applied Microbiology and Biotechnology 49, 770-777.
15. Gomes, J., Gomes, I. and Steiner, W. (2003). Highly thermostable amylase and pullulanase of the extreme thermophilic eubacterium Rhodothermus marinus: Production and partial characterization. Bioresource Technology 90, 207-214.
16. Haki, G. D. and Rakshit, S. K. (2003). Developments in industrially important thermostable enzymes: A review. Bioresource Technology 89, 17-34.
17. Hyun, H. H. and Zeikus, J. G. (1985). General and biochemical characterization of thermostable extracellular β-amylase from Clostridium thermosulfurogenes. Applied and Environmental Microbiology 49, 1162-1167.
18. Kim, T. U., Gu, B. G., Jeong, J. Y., Byun, S. M. and Shin, Y. C. (1995). Purification and characterization of maltotetraose forming alkaline Bacillus strain GM 8901. Applied and Environmental Microbiology 61, 3105-3112.
19. Kwak, Y., Akeba, T. and Kudo, T. (1998). Purification and characterization of α-amylase from hyperthermophilic archaeon Thermococcus profundus, which hydrolyses both α-1,4 and α-1,6-glucosidic linkages. Journal of Fermentation and Bioengineering 86, 363-367.
20. LaemmLi, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.
21. Lin, L., Chyau,C. C. and Hsu, W. H. (1998). Production and properties of a raw starch-degrading amylase from the thermophilic and alkaliphilic Bacillus sp. TS-23. Biotechnology and Applied Biochemistry 28, 61-68.
22. Leveque, E., Janecek, S., Haye, B. and Belarbi, A. (2000). Thermophilic archael amylolytic enzymes. Enzyme and Microbial Technology 26, 3-14.
23. Lowry, O. H., Rosebrough, N. J., Farr, A. L. and Randall, R. J. (1951). Protein measurement with folin phenol reagent. Journal of Biological Chemistry 193, 265-275.
24. Mathupala, S., Saha, B. C. and Zeikus, J. G. (1990). Substrate competition and specificity at the active site of amylopullulanase from Clostridium thermohydrosulfuricum. Biochemical and Biophysical Research Communications 166, 126-132.
25. Melasniemi, H. (1987). Characterization of α-amylase and pullulanase activities of Clostridium thermohydrosulfuricum. Biochemical Journal 246, 193-197.
26. Miller, G. L. (1959). Use of dinitrosalicylic acid reagent for determination of reducing sugars. Analytical Chemistry 31, 426-428.
27. Mrudula, S., Gopal Reddy and Seenayya,G. (2010). Screening of medium components for production of thermostable amylopullulanase by Clostridium thermosulfurogenes SVM 17 in submerged fermentation using Plackett-Burman Design. Asian Journal of Microbiology Biotechnology and Environmental Sciences 3, 4-9.
28. Mrudula, S. (2010). Optimization of thermostable amylopullulanase production in solid state fermentation by Clostridium thermosulfurogenes SVM 17 through Plackett-Burman and response surface methodological approaches. Malaysian Journal of Microbiology 6(2), 181-195.
29. Mrudula, S., Gopal Reddy and Seenayya,G. (2011). Effect of process paprameters on production of thermostable amylopullulanase by Clostridium thermosulfurogenes SVM 17 under solid state fermentation. Malaysian Journal of Microbiology 7(1), 19-25.
30. Plant, A. R., Morgan, H. W.and Daniel, R. M. (1986). A highly stable pullulanase from Thermus aquaticus YT-1. Enzyme and Microbial Technology 668-672.
31. Plant, A. R., Clemens, R. M., Daniel, R. M. and Morgan, H. W. (1987). Purification and preliminary characterization of an extra cellular pullulanase from Thermoanaerobium Tok6-BI. Applied Microbiology and Biotechnology 26, 427-433.
32. Ramesh, M. V., Podkovyrov, S. M., Lowe S. E. and Zeikus, J. G. (1994). Cloning and sequencing of the Thermoanaerobaterium saccharolyticum B6A-RI apu gene and purification of the amylopullulanase from Escherichia coli. Applied and Environmental Microbiology 60, 94-101.
33. Rama Mohan Reddy, P., Swamy, M. V. and Seenayya, G. (1998). Purification and characterization of thermostable β-amylase and pullulanase from high-yielding Clostridium thermosulfurogenes SV2. World Journal of Microbiology and Biotechnology 14, 89-94.
34. Rudiger, A., Jorgensen, P.L. and Antranikian, G. (1995). Isolation and characterization of a heat stable pullulanase from the hyperthermophilic archaeon Pyrococcus woesei after cloning and expression of its gene in Escherichia coli. Applied and Environmental Microbiology 61, 567-575.
35. Saha, B. C.and Zeikus, J. G. (1987). Biotechnology of maltose syrup production. Process Biochemistry 22, 78-82.
36. Saha, B. C., Mathupala, S. P. and Zeikus, J. G. (1988). Purification and characterization of a highly thermostable novel pullulanase from Clostridium thermohydrosulfuricum. Biochemical Journal 252, 343-348.
37. Saha, B. C., Lamed, R. L., Lee, C. Y. Mathupala, S. P. and Zeikus, J. G. (1990). Characterization of an endo-acting amylopullulanase from Thermoanaerobacter strain B6A. Applied and Environmental Microbiology 56, 881-886.
38. Sata, H., Umeda, M., Kim, C. H., Taniguchi, H. and Maruyama, Y. (1989). Amylase-Pullulanase enzyme produced by B. Circulans F-2. Biochimica et Biophysica Acta 991, 388-394.
39. Saxena, R. K., Dutt, K., Agarwal, L. and Nayyar, P. (2007). A highly thermostable and alkaline amylase from a Bacillus sp. PN5. Bioresource Technology 98, 260-265.
40. Shafiei, M., Zjaee, A. A. and Amoozegar, M. A. (2010). Purification and biochemical characterization of a novel SDS and surfactant stable raw starch digesting and halophilic α-amylase from moderately halophilic bacterium Nesterenkonia sp. strain F. Process Biochemistry 45, 694-699.
41. Shen, G. J., Saha, B. C., Lee, Y. E., Bhatnagar, L and Zeikus, J. G. (1988). Purification and characterization of a novel thermostable β-amylase from Clostridium thermosulfurogenes. Biochemical Journal 254, 835-840.
42. Sivaramakrishnan,S., Gangadharan, D., Madhavan Nampoothiri, K., Soccol, C. R. and Pandey, A. (2006). α-amylases from microbial source-An overview on recent developments. Food Technology and Biotechnology 44, 173-184.
43. Soni, S. K., Sodhi, H. K., Sharma, K., Gupta, J. K. (2005). Production of thermostable α-amylase from Bacillus sp. PS-7 by solid state fermentation and its synergistic use in the hydrolysis of malt starch for alcohol production. Process Biochemistry 40, 525-534.
44. Spreinat, A. and Antranikian, G. (1990). Purification and properties of a thermostable pullulanase from Clostridium thermosulfurogenes EM1 which hydrolyses both α-1,6 and α-1,4 glycosidic Linkages. Applied Microbiology and Biotechnology 33, 511-518.
45. Suzuki, Y. and Imai, T. (1985). Bacillus stearothermophilus KP 1064 Pullulan hydrolase, its assignment to a unique type of maltogenic α-amylase but neither pullulanase nor isopullulanase. Applied Microbiology and Biotechnology 21, 20-26.
46. Swamy, M. V. (1994). A study into maltose and maltooligosaccharides producing thermostable amylases from Clostridium thermosulfurogenes SV9, Ph.D. Thesis, Osmania University, Hyderabad, India.
47. Swamy, M. V. and Seenayya, G. (1996a). Clostridium thermosulfurogenes SV9-A thermophilic amylases producer. Indian Journal of Microbiology 36, 181-184.
48. Swamy, M. V. and Seenayya, G. (1996b). Thermostable pullulanase and α-amylase from Clostridium thermosulfurogenes SV9: Optimization of culture conditions for enzymes production. Process Biochemistry 31, 157-162.
49. Umesh Kumar, S., Rehana, F. and Nand, K. (1990). Production of an extracellular thermostable calcium-inhibited α-amylase by Bacillus licheniformis MY 10. Enzyme and Microbial Technology 12, 20-26.
50. Vieille, C and Zeikus, J. G. (2001). Hyperthermophilic enzymes: Sources, uses, and Molecular mechanisms for thermostability. Microbiology and Molecular Biology Reviews 65, 1-43.
51. Zareian, S., Khajeh, K., Ranjbar, B., Dabirmanesh, B., Ghollasi, M., Mollania, N. (2010). Purification and characterization of a novel amylopullulanase that converts pullulan to glucose, maltose and maltotriose and starch to glucose and maltose. Enzyme and Microbial Technology. 46, 57-63.