Accumulation of p21 proteins at DNA damage sites independent of p53 and core NHEJ factors following irradiation

Biochemical and Biophysical Research Communications

Volumn 412, Issue 1, 2011, Pages 39-43

  Koike, Manabu ^a   Yutoku, Yasutomo ^a,b   Koike, Aki ^a  

^a NATIONAL INSTITUTE OF RADIOLOGICAL SCIENCES   (Japan)

^b CHIBA UNIVERSITY   (Japan)

Author keywords

H2AX; DNA damage; Localization; P21

Indexed keywords

DNA; DOUBLE STRANDED DNA BREAK SENSOR PROTEIN; ENHANCED GREEN FLUORESCENT PROTEIN; HISTONE H2AX; HISTONE H2AX GAMMA; KU ANTIGEN; MUTANT PROTEIN; PROTEIN; PROTEIN KINASE C; PROTEIN P21; PROTEIN P53; UNCLASSIFIED DRUG; XRCC4 PROTEIN;

ANIMAL CELL; APOPTOSIS; ARTICLE; BIOACCUMULATION; CELLULAR DISTRIBUTION; CONTROLLED STUDY; DNA REPAIR; DOUBLE STRANDED DNA BREAK; FEMALE; HUMAN; HUMAN CELL; HUMAN PAPILLOMAVIRUS TYPE 18; IRRADIATION; MOUSE; NONHOMOLOGOUS DNA END JOINING; NONHUMAN; PRIORITY JOURNAL; PROTEIN LOCALIZATION; SENESCENCE;

ANIMALS; CELL LINE; CELL LINE, TRANSFORMED; CELL LINE, TUMOR; CRICETINAE; CYCLIN-DEPENDENT KINASE INHIBITOR P21; DNA BREAKS, DOUBLE-STRANDED; HUMANS; MICE; PROLIFERATING CELL NUCLEAR ANTIGEN; RECOMBINATION, GENETIC; TUMOR SUPPRESSOR PROTEIN P53;

ANIMALIA; HUMAN PAPILLOMAVIRUS - 18;

EID: 80051802623     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2011.07.032     Document Type: Article

References (28)

1. Khanna K.K., Jackson S.P. DNA double-strand breaks: signaling. Repair and the cancer connection. Nat. Genet. 2001, 27:247-254.
2. Mahaney B.L., Meek K., Lees-Miller S.P. Repair of ionizing radiation-induced DNA double-strand breaks by non-homologous end-joining. Biochem. J. 2009, 417:639-650.
3. Tobias F., Durante M., Taucher-Scholz G., Jakob B. Spatiotemporal analysis of DNA repair using charged particle radiation. Mutat. Res. 2010, 704:54-60.
4. Oh K.S., Bustin M., Mazur S.J., Appella E., Kraemer K.H. UV-induced histone H2AX phosphorylation and DNA damage related proteins accumulate and persist in nucleotide excision repair-deficient XP-B cells. DNA Repair 2011, 10:5-15.
5. Rogakou E.P., Pilch D.R., Orr A.H., Ivanova V.S., Bonner W.M. DNA double-stranded breaks induce histone H2AX phosphorylation on serine 139. J. Biol. Chem. 1998, 273:5858-5868.
6. Watters G.P., Smart D.J., Harvey J.S., Austin C.A. H2AX phosphorylation as a genotoxicity endpoint. Mutat. Res. 2009, 679:50-58.
7. Sherr C.J., Roberts J.M. CDK inhibitors: positive and negative regulators of G1-phase progression. Genes Dev. 1999, 13:1501-1512.
8. Dotto G.P. P21(WAF1/Cip1): more than a break to the cell cycle?. Biochim. Biophys. Acta 2000, 1471:M43-M56.
9. Jakob B., Scholz M., Taucher-Scholz G. Characterization of CDKN1A (p21) binding to sites of heavy-ion-induced damage: colocalization with proteins involved in DNA repair. Int. J. Radiat. Biol. 2002, 78:75-88.
10. Perucca P., Cazzalini O., Mortusewicz O., Necchi D., Savio M., Nardo T., Stivala L.A., Leonhardt H., Cardoso M.C., Prosperi E. Spatiotemporal dynamics of p21CDKN1A protein recruitment to DNA-damage sites and interaction with proliferating cell nuclear antigen. J. Cell Sci. 2006, 119:1517-1527.
11. Kumaravel T.S., Bharathy K., Kudoh S., Tanaka K., Kamada N. Expression, localization and functional interactions of Ku70 subunit of DNA-PK in peripheral lymphocytes and Nalm-19 cells after irradiation. Int. J. Radiat. Biol. 1998, 74:481-489.
12. Koike M., Awaji T., Kataoka M., Tsujimoto G., Kartasova T., Koike A., Shiomi T. Differential subcellular localization of DNA-dependent protein kinase components Ku and DNA-PKcs during mitosis. J. Cell Sci. 1999, 112:4031-4039.
13. Koike M., Koike A. Accumulation of Ku80 proteins at DNA double-strand breaks in living cells. Exp. Cell Res. 2008, 314:1061-1070.
14. Koike M., Yutoku Y., Koike A. Establishment of Ku70-deficient lung epithelial cell lines and their hypersensitivity to low-dose X-irradiation. J. Vet. Med. Sci. 2011, 73:549-554.
15. Koike M., Koike A. Establishment and characterization of stable cell lines expressing human Ku80 tagged with enhanced green fluorescent protein. J. Radiat. Res. 2004, 45:119-125.
16. Koike M., Shiomi T., Koike A. Dimerization and nuclear localization of Ku proteins. J. Biol. Chem. 2001, 276:11167-11173.
17. Koike M., Koike A., Sugasawa J., Toyooka T., Ibuki Y. Dynamics of Ku80 in living hamster cells with DNA double-strand breaks induced by chemotherapeutic drugs. J. Vet. Med. Sci. 2010, 72:1405-1412.
18. Li J., Tan J., Zhuang L., Banerjee B., Yang X., Chau J.F., Lee P.L., Hande M.P., Li B., Yu Q. Ribosomal protein S27-like, a p53-inducible modulator of cell fate in response to genotoxic stress. Cancer Res. 2007, 67:11317-11326.
19. Nakanishi M., Robetorye R.S., Pereira-Smith O.M., Smith J.R. The C-terminal region of p21SDI1/WAF1/CIP1 is involved in proliferating cell nuclear antigen binding but does not appear to be required for growth inhibition. J. Biol. Chem. 1995, 270:17060-17063.
20. Wei L., Lan L., Hong Z., Yasui A., Ishioka C., Chiba N. Rapid recruitment of BRCA1 to DNA double-strand breaks is dependent on its association with Ku80. Mol. Cell Biol. 2008, 28:7380-7393.
21. Balajee A.S., Geard C.R. Chromatin-bound PCNA complex formation triggered by DNA damage occurs independent of the ATM gene product in human cells. Nucleic Acids Res. 2001, 29:1341-1351.
22. Park S.J., Ciccone S.L., Freie B., Kurimasa A., Chen D.J., Li G.C., Clapp D.W., Lee S.H. A positive role for the Ku complex in DNA replication following strand break damage in mammals. J. Biol. Chem. 2004, 279:6046-6055.
23. Koike M., Mashino M., Sugasawa J., Koike A. Histone H2AX phosphorylation independent of ATM after X-irradiation in mouse liver and kidney in situ. J. Radiat. Res. 2008, 49:445-449.
24. Bhogal N., Jalali F., Bristow R.G. Microscopic imaging of DNA repair foci in irradiated normal tissues. Int. J. Radiat. Biol. 2009, 85:732-746.
25. Svetlova M.P., Solovjeva L.V., Tomilin N.V. Mechanism of elimination of phosphorylated histone H2AX from chromatin after repair of DNA double-strand breaks. Mutat. Res. 2010, 685:54-60.
26. Cazzalini O., Donà F., Savio M., Tillhon M., Maccario C., Perucca P., Stivala L.A., Scovassi A.I., Prosperi E. P21CDKN1A participates in base excision repair by regulating the activity of poly(ADP-ribose) polymerase-1. DNA Repair (Amst). 2010, 9:627-635.
27. Wang M., Wu W., Wu W., Rosidi B., Zhang L., Wang H., Iliakis G. PARP-1 and Ku compete for repair of DNA double strand breaks by distinct NHEJ pathways. Nucleic Acids Res. 2006, 34:6170-6182.
28. Paddock M.N., Bauman A.T., Higdon R., Kolker E., Takeda S., Scharenberg A.M. Competition between PARP-1 and Ku70 control the decision between high-fidelity and mutagenic DNA repair. DNA Repair (Amst). 2011, 10:338-343.