Development of a serum-free human cornea construct for in vitro drug absorption studies: The influence of varying cultivation parameters on barrier characteristics

International Journal of Pharmaceutics

Volumn 416, Issue 1, 2011, Pages 268-279

  Hahne, Matthias ^a   Reichl, Stephan ^a  

^a TECHNICAL UNIVERSITY OF BRAUNSCHWEIG   (Germany)

Author keywords

Cell culture; Cornea; Drug absorption; In vitro model; Serum free cultivation

Indexed keywords

DEXTRAN; FLUORESCEIN ISOTHIOCYANATE; FLUORESCEIN SODIUM; RHODAMINE B;

ADULT; ARTICLE; CELL COUNT; CELL CULTURE; CELL FUNCTION; CELL LINE; CELL SUSPENSION; CORNEA; CORNEA CELL; CORNEA EPITHELIUM; CORNEA PERMEABILITY; DRUG ABSORPTION; ELECTRIC RESISTANCE; EPITHELIUM CELL; FEMALE; HEMICORNEA; HUMAN; HUMAN CELL; HUMAN CORNEAL EPITHELIAL CELL; KERATINOCYTE; MACROMOLECULE; MICROSCOPY; PRIORITY JOURNAL; TRANSEPITHELIAL ELECTRICAL RESISTANCE;

ABSORPTION; ANIMALS; CELL CULTURE TECHNIQUES; CELL LINE, TRANSFORMED; CULTURE MEDIA; ELECTRIC IMPEDANCE; EPITHELIUM, CORNEAL; HUMANS; MOLECULAR IMAGING; OPHTHALMIC SOLUTIONS; RABBITS; REPRODUCIBILITY OF RESULTS; SERUM; SWINE;

EID: 80051799721     PISSN: 03785173     EISSN: 18733476     Source Type: Journal    
DOI: 10.1016/j.ijpharm.2011.07.004     Document Type: Article

References (53)

1. K. Araki-Sasaki, Y. Ohashi, T. Sasabe, K. Hayashi, H. Watanabe, Y. Tano, and H. Handa An SV40-immortalized human corneal epithelial cell line and its characterization Invest. Ophthalmol. Vis. Sci. 36 1995 614 621
2. J. Barar, A.R. Javadzadeh, and Y. Omidi Ocular novel drug delivery: impacts of membranes and barriers Expert Opin. Drug Deliv. 5 2008 567 581 (Pubitemid 351877434)
3. M.P. Beales, J.L. Funderburgh, J.V. Jester, and J.R. Hassell Proteoglycan synthesis by bovine keratocytes and corneal fibroblasts: maintenance of the keratocyte phenotype in culture Invest. Ophthalmol. Vis. Sci. 40 1999 1658 1663 (Pubitemid 29297138)
4. U. Becker, C. Ehrhardt, U.F. Schaefer, H.J. Gukasyan, K.J. Kim, V.H. Lee, and C.M. Lehr Tissue distribution of moxaverine-hydrochloride in the rabbit eye and plasma J. Ocul. Pharmacol. Ther. 21 2005 210 216 (Pubitemid 40934699)
5. U. Becker, C. Ehrhardt, N. Daum, C. Baldes, U.F. Schaefer, K.W. Ruprecht, K.J. Kim, and C.M. Lehr Expression of ABC-transporters in human corneal tissue and the transformed cell line, HCE-T J. Ocul. Pharmacol. Ther. 23 2007 172 181 (Pubitemid 46642159)
6. U. Becker, C. Ehrhardt, M. Schneider, L. Muys, D. Gross, K. Eschmann, U.F. Schaefer, and C.M. Lehr A comparative evaluation of corneal epithelial cell cultures for assessing ocular permeability Altern. Lab. Anim. 36 2008 33 44 (Pubitemid 351439797)
7. V.M. Borderie, N. Mourra, and L. Laroche Influence of fetal calf serum, fibroblast growth factors, and hepatocyte growth factor on three-dimensional cultures of human keratocytes in collagen gel matrix Graefes Arch. Clin. Exp. Ophthalmol. 237 1999 861 869 (Pubitemid 29438469)
8. J.E. Chang, S.K. Basu, and V.H. Lee Air-interface condition promotes the formation of tight corneal epithelial cell layers for drug transport studies Pharm. Res. 17 2000 670 676 (Pubitemid 30670631)
9. L. Chen, N. Ebihara, K. Fujiki, and A. Murakami Expression and distribution of junctional adhesion molecule-1 in the human cornea Jpn. J. Ophthalmol. 51 2007 405 411
10. V.R. Goskonda, M.A. Khan, C.M. Hutak, and I.K. Reddy Permeability characteristics of novel mydriatic agents using an in vitro cell culture model that utilizes SIRC rabbit corneal cells J. Pharm. Sci. 88 1999 180 184 (Pubitemid 29084728)
11. V.R. Goskonda, R.A. Hill, M.A. Khan, and I.K. Reddy Permeability of chemical delivery systems across rabbit corneal (SIRC) cell line and isolated corneas: a comparative study Pharm. Dev. Technol. 5 2000 409 416 (Pubitemid 30602849)
12. M. Hahne, and S. Reichl Simulation of corneal epithelial injuries by mechanical and corrosive damage: Influence of fetal bovine serum and dexpanthenol on epithelial regeneration in a cell culture model Ophthalmologe 107 2010 529 532 534-526
13. M. Hornof, E. Toropainen, and A. Urtti Cell culture models of the ocular barriers Eur. J. Pharm. Biopharm. 60 2005 207 225 (Pubitemid 40804562)
14. A.J. Huang, S.C. Tseng, and K.R. Kenyon Paracellular permeability of corneal and conjunctival epithelia Invest. Ophthalmol. Vis. Sci. 30 1989 684 689 (Pubitemid 19117189)
15. K. Järvinen, T. Järvinen, and A. Urtti Ocular absorption following topical delivery Adv. Drug Deliv. Rev. 16 1995 3 19
16. J.V. Jester, and J. Ho-Chang Modulation of cultured corneal keratocyte phenotype by growth factors/cytokines control in vitro contractility and extracellular matrix contraction Exp. Eye Res. 77 2003 581 592 (Pubitemid 37188733)
17. K. Kawazu, H. Shiono, H. Tanioka, A. Ota, T. Ikuse, H. Takashina, and Y. Kawashima Beta adrenergic antagonist permeation across cultured rabbit corneal epithelial cells grown on permeable supports Curr. Eye Res. 17 1998 125 131 (Pubitemid 28073776)
18. S.D. Klyce, and C.E. Crosson Transport processes across the rabbit corneal epithelium: a review Curr. Eye Res. 4 1985 323 331 (Pubitemid 15123153)
19. J.A. Ko, R. Yanai, and T. Nishida Up-regulation of ZO-1 expression and barrier function in cultured human corneal epithelial cells by substance P FEBS Lett. 583 2009 2148 2153
20. F.E. Kruse, and S.C. Tseng Serum differentially modulates the clonal growth and differentiation of cultured limbal and corneal epithelium Invest. Ophthalmol. Vis. Sci. 34 1993 2976 2989 (Pubitemid 23269153)
21. A.K. Manzer, S. Lombardi-Borgia, M. Schafer-Korting, J. Seeber, M. Zorn-Kruppa, and M. Engelke SV40-transformed human corneal keratocytes: optimisation of serum-free culture conditions ALTEX 26 2009 33 39
22. Y. Minami, H. Sugihara, and S. Oono Reconstruction of cornea in three-dimensional collagen gel matrix culture Invest. Ophthalmol. Vis. Sci. 34 1993 2316 2324 (Pubitemid 23191261)
23. N. Nagai, M. Inomata, and Y. Ito Contribution of aldehyde dehydrogenase 3A1 to disulfiram penetration through monolayers consisting of cultured human corneal epithelial cells Biol. Pharm. Bull. 31 2008 1444 1448 (Pubitemid 351930666)
24. T. Nakamura, M. Yamada, M. Teshima, M. Nakashima, H. To, N. Ichikawa, and H. Sasaki Electrophysiological characterization of tight junctional pathway of rabbit cornea treated with ophthalmic ingredients Biol. Pharm. Bull. 30 2007 2360 2364 (Pubitemid 350223743)
25. E.J. Orwin, and A. Hubel In vitro culture characteristics of corneal epithelial, endothelial, and keratocyte cells in a native collagen matrix Tissue Eng. 6 2000 307 319 (Pubitemid 30655941)
26. W.G. Pond, and K.A. Houpt The Biology of the Pig 1978 Cornell University Press Ithaca and London
27. M.R. Prausnitz, and J.S. Noonan Permeability of cornea, sclera, and conjunctiva: a literature analysis for drug delivery to the eye J. Pharm. Sci. 87 1998 1479 1488
28. V.P. Ranta, M. Laavola, E. Toropainen, K.S. Vellonen, A. Talvitie, and A. Urtti Ocular pharmacokinetic modeling using corneal absorption and desorption rates from in vitro permeation experiments with cultured corneal epithelial cells Pharm. Res. 20 2003 1409 1416 (Pubitemid 37164049)
29. S. Reichl Cell culture models of the human cornea - a comparative evaluation of their usefulness to determine ocular drug absorption in-vitro J. Pharm. Pharmacol. 60 2008 299 307 (Pubitemid 351269816)
30. S. Reichl, and U. Becker Cell culture models of the corneal epithelium and reconstructed cornea equivalents for in vitro drug absorption studies C. Ehrhardt, K.J. Kim, Drug Absorption Studies - In Situ, In Vitro and In Silico Models first ed. 2008 Springer New York 283 306
31. S. Reichl, and C.C. Müller-Goymann Development of an organotypic corneal construction as an in vitro model for permeability studies Ophthalmologe 98 2001 853 858 (Pubitemid 32905033)
32. S. Reichl, and C.C. Müller-Goymann The use of a porcine organotypic cornea construct for permeation studies from formulations containing befunolol hydrochloride Int. J. Pharm. 250 2003 191 201 (Pubitemid 35447949)
33. S. Reichl, J. Bednarz, and C.C. Müller-Goymann Human corneal equivalent as cell culture model for in vitro drug permeation studies Br. J. Ophthalmol. 88 2004 560 565 (Pubitemid 38437881)
34. S. Reichl, S. Dohring, J. Bednarz, and C.C. Müller-Goymann Human cornea construct HCC - an alternative for in vitro permeation studies? A comparison with human donor corneas Eur. J. Pharm. Biopharm. 60 2005 305 308 (Pubitemid 40804570)
35. S. Reichl, C. Kölln, M. Hahne, and J. Verstraelen In vitro cell culture models to study the corneal drug absorption Expert Opin. Drug Metab. Toxicol. 7 2011 559 578
36. Y. Rojanasakul, L.Y. Wang, M. Bhat, D.D. Glover, C.J. Malanga, and J.K. Ma The transport barrier of epithelia: a comparative study on membrane permeability and charge selectivity in the rabbit Pharm. Res. 9 1992 1029 1034
37. K. Sakanaka, K. Kawazu, K. Nishida, J. Nakamura, M. Nakashima, T. Nakamura, A. Oshita, N. Ichikawa, and H. Sasaki Transport of timolol and tilisolol in rabbit corneal epithelium Biol. Pharm. Bull. 29 2006 2143 2147 (Pubitemid 44510826)
38. A.I. Schneider, K. Maier-Reif, and T. Graeve The use of an in vitro cornea for predicting ocular toxicity In Vitro Toxicol. 10 1997 309 318
39. M. Scholz, J.E. Lin, V.H. Lee, and S. Keipert Pilocarpine permeability across ocular tissues and cell cultures: influence of formulation parameters J. Ocul. Pharmacol. Ther. 18 2002 455 468 (Pubitemid 37420254)
40. J.W. Seeber, M. Zorn-Kruppa, S. Lombardi-Borgia, H. Scholz, A.K. Manzer, B. Rusche, M. Schafer-Korting, and M. Engelke Characterisation of human corneal epithelial cell cultures maintained under serum-free conditions Altern. Lab. Anim. 36 2008 569 583
41. M. Sosnova-Netukova, P. Kuchynka, and J.V. Forrester The suprabasal layer of corneal epithelial cells represents the major barrier site to the passive movement of small molecules and trafficking leukocytes Br. J. Ophthalmol. 91 2007 372 378 (Pubitemid 46586538)
42. E. Steed, M.S. Balda, and K. Matter Dynamics and functions of tight junctions Trends Cell Biol. 20 2010 142 149
43. R.V. Tak, D. Pal, H. Gao, S. Dey, and A.K. Mitra Transport of acyclovir ester prodrugs through rabbit cornea and SIRC-rabbit corneal epithelial cell line J. Pharm. Sci. 90 2001 1505 1515 (Pubitemid 32979745)
44. S. Tegtmeyer, I. Papantoniou, and C.C. Muller-Goymann Reconstruction of an in vitro cornea and its use for drug permeation studies from different formulations containing pilocarpine hydrochloride Eur. J. Pharm. Biopharm. 51 2001 119 125 (Pubitemid 32162490)
45. S. Tegtmeyer, S. Reichl, and C.C. Muller-Goymann Cultivation and characterization of a bovine in vitro model of the cornea Pharmazie 59 2004 464 471 (Pubitemid 38828636)
46. E. Toropainen, V.P. Ranta, A. Talvitie, P. Suhonen, and A. Urtti Culture model of human corneal epithelium for prediction of ocular drug absorption Invest. Ophthalmol. Vis. Sci. 42 2001 2942 2948 (Pubitemid 33035294)
47. E. Toropainen, V.P. Ranta, K.S. Vellonen, J. Palmgren, A. Talvitie, M. Laavola, P. Suhonen, K.M. Hamalainen, S. Auriola, and A. Urtti Paracellular and passive transcellular permeability in immortalized human corneal epithelial cell culture model Eur. J. Pharm. Sci. 20 2003 99 106 (Pubitemid 37103237)
48. P. Van Der Bijl, A.H. Engelbrecht, A.D. Van Eyk, and D. Meyer Comparative permeability of human and rabbit corneas to cyclosporin and tritiated water J. Ocul. Pharmacol. Ther. 18 2002 419 427 (Pubitemid 37420251)
49. A.D. Van Eyk, H.I. Seifart, D. Meyer, and P. Van der Bijl In vitro transcorneal diffusion of the antimicrobial macrolides azithromycin and clarithromycin and the impact on microbial keratitis Cornea 28 2009 441 446
50. Wang, S.; Jiang, T.; Wang, Z.; 2008. Permeability and anticataract effects of a topical ocular drug delivery system of disulfiram, BioMedical engineering and informatics: new development and the future - Proceedings of the 1st International Conference on BioMedical Engineering and Informatics. Vol. 1. BMEI; pp. 596-600.
51. C.D. Xiang, M. Batugo, D.C. Gale, T. Zhang, J. Ye, C. Li, S. Zhou, E.Y. Wu, and E.Y. Zhang Characterization of human corneal epithelial cell model as a surrogate for corneal permeability assessment: metabolism and transport Drug Metab. Dispos. 37 2009 992 998
52. T. Zhang, C.D. Xiang, D. Gale, S. Carreiro, E.Y. Wu, and E.Y. Zhang Drug transporter and cytochrome P450 mRNA expression in human ocular barriers: implications for ocular drug disposition Drug Metab. Dispos. 36 2008 1300 1307 (Pubitemid 351929314)
53. M. Zorn-Kruppa, S. Tykhonova, G. Belge, J. Bednarz, H.A. Diehl, and M. Engelke A human corneal equivalent constructed from SV40-immortalised corneal cell lines Altern. Lab. Anim. 33 2005 37 45 (Pubitemid 40476881)