Proteoglycan synthesis by bovine keratocytes and corneal fibroblasts: Maintenance of the keratocyte phenotype in culture

Investigative Ophthalmology and Visual Science

Volumn 40, Issue 8, 1999, Pages 1658-1663

  Beales, Marianne P ^a,b   Funderburgh, James L ^c   Jester, James V ^d   Hassell, John R ^a,b  

^a SHRINERS HOSPITALS FOR CHILDREN   (United States)

^b UNIVERSITY OF SOUTH FLORIDA   (United States)

^c KANSAS STATE UNIVERSITY   (United States)

^d UNIVERSITY OF TEXAS AT DALLAS   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL CELL; ARTICLE; CATTLE; CELL ACTIVATION; CELL ISOLATION; CELL PROLIFERATION; CELL VIABILITY; CONTROLLED STUDY; CORNEA CELL; CORNEA STROMA; FIBROBLAST; MYOFIBROBLAST; NONHUMAN; PRIORITY JOURNAL; PROTEOGLYCAN SYNTHESIS;

ANIMALS; BLOOD; CATTLE; CELL DIVISION; CELLS, CULTURED; CHONDROITIN SULFATES; CHROMATOGRAPHY, AFFINITY; CORNEAL STROMA; CULTURE MEDIA, SERUM-FREE; DNA; FIBROBLASTS; KERATAN SULFATE; ORGAN CULTURE TECHNIQUES; PHENOTYPE; PROTEOGLYCANS;

EID: 0033064917     PISSN: 01460404     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (33)

1. Birk DE, Fitch JM, Linsenmayer TF. Organization of collagen types I and V in the embryonic chicken cornea. Invest Ophthalmol Vis Sci. 1986;27:1470-1477.
2. Cintron C, Hong B-S. Heterogeneity of collagens in rabbit cornea: type VI collagen. Invest Ophthalmol Vis Sci. 1988;29:760-766.
3. Fitch JM, Birk DE, Linsenmayer C, Linsenmayer TF. Stromal assemblies containing collagen types IV and VI and fibronectin in the developing embryonic avian cornea. Dev Biol. 1991;144:379-391.
4. Wessel H, Anderson S, Fite D, Halvas E, Hempel J, SundarRaj N. Type XII collagen contributes to diversities in human corneal and limbal extracellular matrices. Invest Ophthalmol Vis Sci. 1997;38: 2408-2422.
5. Li W, Vergnes J-P, Cornuet PK, Hassell JR. cDNA clone to chick cornea chondroitin/dermatan sulfate proteoglycan reveals identity to decorin. Arch Biochem Biopbys. 1992;296:190-197.
6. Chakravarti S, Stalling RL, SundarRaj N, Cornuet PK, Hassell JR. Primary structure of human lumican (keratan sulfate proteoglycan) and localization of the gene (Lum) to chromosome 12q21.3-q22. Genomics. 1995;27:481-488.
7. Corpuz LM, Funderburgh JL, Funderburgh ML, Bottomley GS, Prakash S, Conrad, GW. Molecular cloning and tissue distribution of keratocan: bovine corneal keratan sulfate proteoglycan 37A. J Biol Chem. 1996;271:9759-9763.
8. Funderburgh JL, Corpuz, LM, Roth, Funderburgh ML, Tasheva ED, Conrad GW. Mimecan, the 25-kDa corneal keratan sulfate proteoglycan, is a product of the gene producing osteoglycin. J Biol Chem. 1997;272:28089-28095.
9. Coulombre AJ, Coulombre JL. Corneal development, I: corneal transparency. J Cell Comp Physiol. 1958;51:1-11.
10. Hassell JR, Cintron C, Kublin C, Newsome DA. Proteoglycan changes during restoration of transparency in corneal scars. Arch Biochem Biophys. 1983;222:362-369.
11. Klintworth K, Smith CF. Abnormalities of proteoglycans and glycoproteins synthesized by corneal organ cultures derived from patients with macular corneal dystrophy. Lab Invest. 1983;48: 603-612.
12. Nakazawa K, Hassell JR, Hascall VC, et al. Defective processing of keratan sulfate in macular corneal dystrophy. J Biol Chem. 1984; 259:13751-13757.
13. Chakravarti S, Magnuson T, Lass JH, Jepsen KJ, LaMantia C, Carroll H. Lumican regulates collagen fibril assembly: skin fragility and corneal opacity in the absence of lumican. J Cell Biol. 1998;141: 1277-1286.
14. Danielson KG, Baribault H, Holmes DF, Graham H, Kadler KE, Iozzo RV. Targeted disruption of decorin leads to abnormal collagen fibril morphology and skin fragility. J Cell Biol. 1997;136:729-743.
15. Hassell JR, Newsome DA, Hascall VC. Characterization and biosynthesis of proteoglycans of corneal stroma from rhesus monkey. J Biol Chem. 1979;254:12346-12354.
16. Midura RJ, Hascall VC. Analysis of the proteoglycans synthesized by corneal expiants from embryonic chicken. J Biol Chem. 1989; 264:1423-1430.
17. Hassell JR, Schrecengost PK, Rada JA, SundarRaj N, Sossi G, Thoft RA. Biosynthesis of stromal matrix proteoglycans and basement membrane components by human corneal fibroblasts. Invest Ophthalmol Vis Sci. 1992;33:547-557.
18. Dahl IMS. Biosynthesis of proteoglycans and hyaluronate in rabbit corneal fibroblast cultures: variation with age of the cell line and effect of foetal calf serum. Exp. Eye Res. 1981;32:419-433.
19. Yue BYJT, Baum JL, Silbert JE. The synthesis of glycosaminoglycans by cultures of rabbit corneal endothelial and stromal cells. J Biochem. 1976;158:567.
20. Funderburgh JL, Funderburgh ML, Mann MM, Prakash S, Conrad GW. Synthesis of corneal keratan sulfate proteoglycans by bovine keratocytes in vitro. J Biol Chem. 1996;271:31431-31436.
21. Masur SK, Cheung JKH, Antohi S. Identification of integrins in cultured corneal fibroblasts and in isolated keratocytes. Invest Ophthalmol Vis Sci. 1993:43:2690-2698.
22. Masur SK, Dewal HS, Dinh TT, Erenburg I, Petridou S. Myofibroblasts differentiate from fibroblasts when plated at low density. Proc Natl Acad Sci., USA. 1996;93:4219-4223.
23. Jester JV, Barry PA, Lind J, Petroll WM, Garana R, Cavanagh HD. Corneal keratocytes: in situ and in vitro organization of cytoskeletal contractile proteins. Invest Ophthalmol Vis Sci. 1994;35:730-743.
24. Petridou S, Masur SK. Immunodetection of connexins and cadherins in corneal fibroblasts and myofibroblasts. Invest Ophthalmol Vis Sci. 1996;37:1740-1748.
25. Jester JV, Barry-Lane PA, Cavanagh HD, Petroll WM. Induction of α-smooth muscle actin expression and myofibroblast transformation in cultured corneal keratocytes. Cornea. 1996;15:505-516.
26. Müller LJ, Pels L, Vrensen GFJM. Novel aspects of the ultrastructural organization of human corneal keratocytes. Invest Ophthalmol Vis Sci. 1995;36:2557-2567.
27. Schrecengost PK, Blochberger TC, Hassell JR. Identification of chick corneal keratan sulfate proteoglycan precursor protein in whole corneas and in cultured corneal fibroblasts. Arch Biochem Biophys. 1992;292:54-61.
28. Nakazawa K, Monta A, Nakano H, Mano C, Tozawa N. Keratan sulfate synthesis by corneal stromal cells within three-dimensional collagen gel cultures. J Biochem. 1996;120:117-125.
29. Johnson-Muller B, Gross J. Regulation of corneal collagenase production: epithelial-stromal cell interactions. Proc Natl Acad Sci., USA. 1978:75-4417-4421.
30. Wilson SE, He YG, Weng J, et al. Epithelial injury induces keratocyte apoptosis: hypothesized role for the interleukin 1 system in the modulation of corneal tissue organization and wound healing. Exp Eye Res. 1996;62:325-328.
31. Wilson SE. Molecular cell biology for the refractive corneal surgeon: programmed cell death and wound healing. J Refract Surg. 1997;13:171-176.
32. Garana RMR, Retroll WM, Chen WT, et al. Radial keratotomy, II: role of the myofibroblasts in corneal wound contraction. Invest Ophthalmol Vis Sci. 1992;33:3271-3282.
33. Jester JV, Petroll WM, Barry PA, Cavanagh HD. Expression of a-actin during corneal stromal wound healing. Invest Ophthalmol Vis Sci. 1995;36:809-819.