Expression of ABC-transporters in human corneal tissue and the transformed cell line, HCE-T

Journal of Ocular Pharmacology and Therapeutics

Volumn 23, Issue 2, 2007, Pages 172-181

  Becker, Ulrich ^a   Ehrhardt, Carsten ^a,b   Daum, Nicole ^a   Baldes, Christiane ^a   Schaefer, Ulrich F ^a   Ruprecht, Klaus W ^c   Kim, Kwang Jin ^d   Lehr, Claus Michael ^a  

^a SAARLAND UNIVERSITY   (Germany)

^b TRINITY COLLEGE DUBLIN   (Ireland)

^c SAARLAND UNIVERSITY   (Germany)

^d UNIVERSITY OF SOUTHERN CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ABC TRANSPORTER; BREAST CANCER RESISTANCE PROTEIN; GLYCOPROTEIN P; LUNG RESISTANCE PROTEIN; MULTIDRUG RESISTANCE PROTEIN 1; MULTIDRUG RESISTANCE PROTEIN 2; RHODAMINE 123;

ARTICLE; CELL LINE; CELL TRANSFORMATION; CONTROLLED STUDY; CORNEA EPITHELIUM; HUMAN; HUMAN TISSUE; IMMUNOFLUORESCENCE MICROSCOPY; PROTEIN EXPRESSION; PROTEIN FUNCTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SIGNAL DETECTION;

ANALYSIS OF VARIANCE; ATP-BINDING CASSETTE TRANSPORTERS; BIOLOGICAL TRANSPORT; CACO-2 CELLS; CELL LINE; CORNEA; CYCLOSPORINE; EPITHELIUM, CORNEAL; GENE EXPRESSION PROFILING; HUMANS; MICROSCOPY; MODELS, BIOLOGICAL; PERMEABILITY; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RHODAMINE 123; RNA, MESSENGER;

EID: 34247388751     PISSN: 10807683     EISSN: None     Source Type: Journal    
DOI: 10.1089/jop.2006.0095     Document Type: Article

References (33)

1. Ahmed, I., and Patton, T.F. Importance of the non-corneal absorption route in topical ophthalmic drug delivery. Invest. Ophthalmol. Vis. Sci. 26:584-587, 1985.
2. Klyce, S.D., and Crosson, C.E. Transport processes across the rabbit corneal epithelium: A review. Curr. Eye Res. 4:323-331, 1985.
3. Huang, H.S., Schoenwald, R.D., and Lach, J.L. Corneal penetration behavior of beta-blocking agents II: Assessment of barrier contributions. J. Pharm. Sci. 72:1272-1279, 1983.
4. Huang, A.J., Tseng, S.C., and Kenyon, K.R. Paracellular permeability of corneal and conjunctival epithelia. Invest. Ophthalmol. Vis. Sci. 30:684-689, 1989.
5. Shitara, Y., Horie, T., and Sugiyama, Y. Transporters as a determinant of drug clearance and tissue distribution. Eur. J. Pharm. Sci. 27:425-446, 2006.
6. Glavinas, H., Krajcsi, P., Cserepes, J., et al. The role of ABC-transporters in drug resistance, metabolism, and toxicity. Curr. Drug Deliv. 1:27-42, 2004.
7. Gottesman, M.M., and Pastan, I. The multidrug transporter, a double edged sword. J. Biol. Chem. 263:12163-12166, 1988.
8. Kahn, C.R., Young, E., Lee, I.H., et al. Human corneal epithelial primary cultures and cell lines with extended life span: In vitro model for ocular studies. Invest. Ophthalmol. Vis. Sci. 34:3429-3441, 1993.
9. Hornof, M., Toropainen, E., and Urtti, A. Cell culture models of the ocular barriers. Eur. J. Pharm. Biopharm. 60:207-225, 2005.
10. Araki-Sasaki, K., Ohashi, Y., Sasabe, T., et al. An SV40-immortalized human corneal epithelial cell line and its characterization. Invest. Ophthalmol. Vis. Sci. 36:614-621, 1995.
11. Chang, J.E., Basu, S.K., and Lee, V.H.L. Air-interface condition promotes the formation of tight corneal epithelial cell layers for drug transport studies. Pharm. Res. 17:670-676, 2000.
12. Offord, E.A., Sharif, N.A., Mace, K., et al. Immortalized human corneal epithelial cells for ocular toxicity and inflammation studies. Invest. Ophthalmol. Vis. Sci. 40:1091-1101, 1999.
13. Mohan, R.R., Possin, D.E., Sinha, S., et al. Development of genetically engineered tet HPV16-E6/E7 transduced human corneal epithelial clones having tight regulation of proliferation and normal differentiation. Exp. Eye Res. 77:395-407, 2003.
14. Hutak, C.M., Kavanagh, M.E., Reddy, I.K., et al. Growth pattern of SIRC rabbit corneal cells in microwell inserts. J. Toxicol. Cut. Ocul. Toxicol. 16:145-156, 1997.
15. Toropainen, E., Ranta, V., Vellonen, K., et al. Paracellular and passive transcellular permeability in immortalized human corneal epithelial cell culture model. Eur. J. Pharm. Sci. 20:99-106, 2003.
16. Zorn-Kruppa, M., Tykhonova, S., Beige, G., et al. A human corneal equivalent constructed from SV40-immortalised corneal cell lines. ATLA 33:37-45, 2005.
17. Toropainen, E., Ranta, V., Talvitie, A., et al. Culture model of human corneal epithelium for prediction of ocular drug absorption. Invest. Ophthalmol. Vis. Sci. 42:2942-2948, 2001.
18. Taipalensuu, J., Tornblom, H, Lindberg, G., et al. Correlation of gene expression of ten drug efflux proteins of the ATP-binding cassette transporter family in normal human jejunum and in human intestinal epithelial Caco-2 cell monolayers. J. Pharmacol. Exp. Ther. 299:164-170, 2001.
19. Steimer, A., Haltner, E., Lehr, C.M. Cell culture models of the respiratory tract relevant to pulmonary drug delivery. J. Aerosol Med. 18:137-182, 2005.
20. Grass, G.M., and Sweetana, S.A. In vitro measurement of gastrointestinal tissue permeability using a new diffusion cell. Pharm. Res. 5:372-376, 1988.
21. Saha, P., Yang, J.J., and Lee, V.H.L. Existence of a P-glycoprotein drug efflux pump in cultured rabbit conjunctival epithelial cells. Invest. Ophthalmol. Vis. Sci. 39:1221-1226, 1998.
22. Yang, J.J., Kim, K.J., and Lee, V.H.L. Role of P-glycoprotein in restricting propanolol transport in cultured rabbit conjunctival epithelial cell layers. Pharm. Res. 17:533-538, 2000.
23. Merriman-Smith, B.R., Young, M.A., Jacobs, M.D., et al. Molecular identification of p-glycoprotein: A role in lens circulation? Invest. Ophthalmol. Vis. Sci. 43:3008-3015, 2002.
24. Kennedy, B.G., and Mangini, N.J. P-glycoprotein expression in human retinal pigment epithelium. Mol. Vis. 8:422-430, 2002.
25. Kawazu, K., Yamada, K., Nakamura, M., et al. Characterization of cyclosporin A transport in cultured rabbit corneal epithelial cells: P-glycoprotein transport activity and binding to cyclophilin. Invest. Ophthalmol. Vis. Sci. 40:1738-1744, 1999.
26. Dey, S., Patel, J., Anand, B.S., et al. Molecular evidence and functional expression of P-glycoprotein (MDR1) in human and rabbit cornea and corneal epithelial cell lines. Invest. Ophthalmol. Vis. Sci. 44:2909-2918, 2003.
27. Katragadda, S., Talluri, R.S., and Mitra, A.K. Modulation of P-glycoprotein-mediated efflux by prodrug derivatization: An approach involving peptide transporter-mediated influx across rabbit cornea. J. Ocul. Pharmacol. Ther. 22:110-120, 2006.
28. Esser, J., Esser, P., Mietz, H., et al. Multidrug resistance-associated proteins in glaucoma surgery. Graefe's Arch. Clin. Exp. Ophthalmol. 238:727-732, 2000.
29. Aukunuru, J.V., Sunkara, G., Bandi, N., et al. Expression of multidrug resistance-associated protein in human retinal pigment epithelial cells and its interaction with BAPSG, a novel aldose reductase inhibitor. Pharm. Res. 18:565-572, 2001.
30. Karla, P.K., Pal, D., and Mitra, A.K. Molecular evidence and functional expression of multidrug resistance-associated protein (MRP) in rabbit corneal epithelial cells. Exp. Eye Res. Epub ahead of print, 2006.
31. Schlötzer-Schrehardt, U., and Kruse, F.E. Identification and isolation of limbal stem cells. Exp. Eye Res. 81:247-264, 2005.
32. Reichl, S., Dohring, S., Bednarz, J., et al. Human cornea construct HCC - an alternative for in vitro permeation studies? A comparison with human donor cornea. Eur. J. Pharm. Biopharm. 60:305-308, 2005.
33. Freshney, I.R. The transformed phenotype. In: Freshney, I.R. Culture of Animal Cells. New York: Alan R. Liss Inc., 1987:199-208.