Drug transporter and cytochrome P450 mRNA expression in human ocular barriers: Implications for ocular drug disposition

Drug Metabolism and Disposition

Volumn 36, Issue 7, 2008, Pages 1300-1307

  Zhang, Tao ^a   Xiang, Cathie D ^b   Gale, David ^b   Carreiro, Samantha ^b   Wu, Ellen Y ^b   Zhang, Eric Y ^b  

^a OHIO STATE UNIVERSITY   (United States)

^b PFIZER INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BETA ACTIN; BREAST CANCER RESISTANCE PROTEIN; CYTOCHROME P450; CYTOCHROME P450 1A2; CYTOCHROME P450 2A6; CYTOCHROME P450 2B6; CYTOCHROME P450 2C8; CYTOCHROME P450 2C9; CYTOCHROME P450 2D6; CYTOCHROME P450 2E1; CYTOCHROME P450 3A4; CYTOCHROME P450 3A5; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; MESSENGER RNA; MULTIDRUG RESISTANCE PROTEIN 1; MULTIDRUG RESISTANCE PROTEIN 2; MULTIDRUG RESISTANCE PROTEIN 3; OCTAMER TRANSCRIPTION FACTOR 1; OCTAMER TRANSCRIPTION FACTOR 2; OCTAMER TRANSCRIPTION FACTOR 4; ORGANIC ANION TRANSPORTER; PEPTIDE TRANSPORTER 1; PEPTIDE TRANSPORTER 2;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BLOOD EYE BARRIER; BLOOD RETINA BARRIER; CHOROID; CILIARY BODY; CONTROLLED STUDY; CORNEA; DOG; DRUG ABSORPTION; DRUG CLEARANCE; DRUG DISPOSITION; DRUG DISTRIBUTION; DRUG TRANSPORT; DRUG UPTAKE; GENE EXPRESSION; HUMAN; HUMAN TISSUE; KIDNEY; LIVER; MONKEY; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; RABBIT; RETINA; SMALL INTESTINE; SPECIES DIFFERENCE; TISSUE DISTRIBUTION;

ANIMALS; BASE SEQUENCE; CYTOCHROME P-450 ENZYME SYSTEM; DNA PRIMERS; DOGS; EYE; FEMALE; HAPLORHINI; HUMANS; MALE; PHARMACEUTICAL PREPARATIONS; PHARMACOKINETICS; POLYMERASE CHAIN REACTION; RABBITS; RNA, MESSENGER;

ANIMALIA; CANIS FAMILIARIS; ORYCTOLAGUS CUNICULUS;

EID: 46449098332     PISSN: 00909556     EISSN: 1521009X     Source Type: Journal    
DOI: 10.1124/dmd.108.021121     Document Type: Article

References (14)

1. Anand BS and Mitra AK (2002) Mechanism of corneal permeation of L-valyl ester of acyclovir: targeting the oligopeptide transporter on the rabbit cornea. Pharm Res 19:1194-1202.
2. Aukunuru JV, Sunkara G, Bandi N, Thoreson WB, and Kompella UB (2001)Expression of multidrug resistance-associated protein (MRP) in human retinal pigment epithelial cells and its interaction with BAPSG, a novel aldose reductase inhibitor. Pharm Res 18:565-572.
3. Becker U, Ehrhardt C, Daum N, Baldes C, Schaefer UF, Ruprecht KW, Kim KJ, and Lehr CM (2007) Expression of ABC-transporters in human corneal tissue and the transformed cell line, HCE-T. J Ocul Pharmacol Ther 23:172-181.
4. Dey S, Patel J, Anand BS, Jain-Vakkalagadda B, Kaliki P, Pal D, Ganapathy V, and Mitra AK (2003) Molecular evidence and functional expression of P-glycoprotein (MDR1) in human and rabbit cornea and corneal epithelial cell lines. Invest Ophthalmol Vis Sci 44:2909-2918.
5. Duvvuri S, Majumdar S, and Mitra AK (2004) Role of metabolism in ocular drug delivery. Curr Drug Metab 5:507-515.
6. Hilgendorf C, Ahlin G, Seithel A, Artursson P, Ungell AL, and Karlsson J (2007) Expression of thirty-six drug transporter genes in human intestine, liver, kidney, and organotypic cell lines. Drug Metab Dispos 35:1333-1340.
7. Hornof M, Toropainen E, and Urtti A (2005) Cell culture models of the ocular barriers. Eur J Pharm Biopharm 60:207-225.
8. Lee VH, Chien DS, and Sasaki H (1988) Ocular ketone reductase distribution and its role in the metabolism of ocularly applied levobunolol in the pigmented rabbit. J Pharmacol Exp Ther 246:871-878.
9. Mannermaa E, Vellonen KS, and Urtti A (2006) Drug transport in corneal epithelium and blood-retina barrier: emerging role of transporters in ocular pharmacokinetics. Adv Drug Deliv Rev 58:1136-1163.
10. Ocheltree SM, Keep RF, Shen H, Yang D, Hughes BA, and Smith DE (2003) Preliminary investigation into the expression of proton-coupled oligopeptide transporters in neural retina and retinal pigment epithelium (RPE): lack of functional activity in RPE plasma membranes. Pharm Res 20:1364-1372.
11. Schoenwald RD (2003) Ocular pharmacokinetics and pharmacodynamics, in Ophthalmic Drug Delivery Systems (Mitra AK ed) pp 135-179, Marcel Dekker, New York.
12. Shen H, Ocheltree SM, Hu Y, Keep RF, and Smith DE (2007) Impact of genetic knockout of PEPT2 on cefadroxil pharmacokinetics, renal tubular reabsorption, and brain penetration in mice. Drug Metab Dispos 35:1209-1216.
13. Shirasaki Y (2007) Molecular design for enhancement of ocular penetration. J Pharm Sci, in press.
14. Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A, and Speleman F (2002) Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol 3:RESEARCH0034.