Human corneal equivalent as cell culture model for in vitro drug permeation studies

British Journal of Ophthalmology

Volumn 88, Issue 4, 2004, Pages 560-565

  Reichl, S ^a   Bednarz, J ^b   Muller Goymann C C ^a  

^a TECHNICAL UNIVERSITY OF BRAUNSCHWEIG   (Germany)

^b UNIVERSITY MEDICAL CENTER HAMBURG EPPENDORF   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

BEFUNOLOL; BENZALKONIUM CHLORIDE; EYE DROPS; HYDROCORTISONE; PILOCARPINE; PRESERVATIVE;

ARTICLE; CELL CULTURE; CORNEA; DRUG ABSORPTION; DRUG PENETRATION; ENDOTHELIUM CELL; EPITHELIUM CELL; EYE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; HUMAN CELL; MODEL; PRIORITY JOURNAL; SCANNING ELECTRON MICROSCOPY;

ADRENERGIC BETA-ANTAGONISTS; CORNEA; HUMANS; HYDROCORTISONE; MICROSCOPY, ELECTRON, SCANNING; MIOTICS; OPHTHALMIC SOLUTIONS; PERMEABILITY; PILOCARPINE; PROPANOLAMINES; TISSUE ENGINEERING;

EID: 1842557335     PISSN: 00071161     EISSN: None     Source Type: Journal    
DOI: 10.1136/bjo.2003.028225     Document Type: Article

References (39)

1. Doane MG, Jensen AD, Dohlmann CH. Penetration routes of topically applied eye medications. Am J Ophthalmol 1978;85:383-86.
2. Burstein NL, Anderson JA. Corneal penetration and ocular bioavailability of drugs. J Ocular Pharmacol 1985;1:309-26.
3. Lee VH, Robinson JR. Topical ocular drug delivery: recent developments and future challenges. J Ocular Phamacol 1986;2:67-108.
4. Prausnitz MR, Noonan JS. Permeability of cornea, sclera and conjunctiva: A literature analysis for drug delivery to the eye. J Pharm Sci 1998;87:1479-88.
5. Scholz M, Schrunder S, Gartner S, et al. Ocular drug permeation following experimental excimer laser treatment on the isolated pig eye. J Ocul Pharmacol Ther 2002;18:177-83.
6. Maurice DM, Mishima S. Ocular Pharmakokinetics. In: Sears ML, ed. Pharmacology of the eye. Berlin: Springer-Verlag, 1984:19-116.
7. Goskonda VR, Khan MA, Hutak CM, et al. Permeability characteristics of novel mydriatic agents using an in vitro cell culture model that utilizes SIRC rabbit corneal cells. J Pharm Sci 1999;88:180-4.
8. Goskonda VR, Reddy IK. Permeability of pure enantiomers of timolol across SIRC rabbit corneal cells. Pharm Pharmacol Commun 1999;5:111-15.
9. Kawazu K, Shiono H, Tanioka H, et al. Beta adrenergic antagonist permeation across cultured rabbit corneal epithelial cells grown on permeable supports. Curr Eye Res 1998;17:125-31.
10. Kawazu K, Yamada K, Nakamura M, et al. Characterization of cyclosporin A transport in cultured rabbit corneal epithelial cells: P-glycoprotein transport activity and binding to cyclophilin. Invest Ophthalmol Vis Sci 1999;40:1738-44.
11. Schneider AI, Maier-Reif K, Graeve T. The use of an in vitro cornea for predicting ocular toxicity. In Vitro Toxicol 1997;10:309-18.
12. Saarinen-Savolainen P, Jarvinen T, Araki-Sasaki K, et al. Evaluation of cytotoxicity of various ophthalmic drugs, eye drop excipients and cyclodextrins in an immortalized human corneal epithelial cell line. Pharm Res 1998;15:1275-80.
13. Offord EA, Sharif NA, Mace K, et al. Immortalized human corneal epithelial cells for ocular toxicity and inflammation studies. Invest Ophthalmol Vis Sci 1999;40:1091-101.
14. Clothier R, Orme A, Walker TL, et al. A comparison of three cytotoxicity endpoints in the corneal HCE-T model. ATLA 2000;28:293-302.
15. Chang J, Basu SK, Lee VHL. Air-interface condition promotes the formation of tight corneal epithelial cell layers for drug transport studies. Pharm Res 2000;17:670-76.
16. Scholz M, Lin JE, Lee VHL, et al. Pilocarpine permeability across ocular tissues and cell cultures: influence of formulation parameters. J Ocul Pharmacol Ther 2002;18:455-68.
17. Toropainen E, Ranta V, Talvitie A, et al. Culture model of human corneal epithelium for prediction of ocular drug absorption. Invest Ophthalmol Vis Sci 2001;42:2942-48.
18. Tegtmeyer S, Papontoniou I, Müller-Goymann CC. Reconstruction of an in vitro cornea and its use for drug permeation studies from different formulations containing pilocarpine hydrochloride. Eur J Pharm Biopharm 2001;51:119-25.
19. Reichl S, Müller-Goymann CC. Development of an organotypical cornea construct as an in vitro model for permeation studies. Ophthalmologe 2001;98:853-58.
20. Reichl S, Müller-Goymann CC. Cultivation of a porcine organotypic cornea construct and its use for various in vitro permeation studies. Arch Pharm 2001;334(Suppl 2):73.
21. Reichl S, Müller-Goymann CC. The use of a porcine organotypic cornea construct for permeation studies from formulations containing befunolol hydrochloride. Int J Pharm 2003;250:191-201.
22. Way SE, Hadgraft J. In vitro investigation of the permeation and metabolism of hydrocortisone and hydrocortisone-21-esters. In: Scott RC, Guy RH, Haagraft J, Boddé HE, eds. Prediction of percutaneous penetration. London: BC Technical Services, 1991;2.
23. Zieske JD, Mason VS, Wasson ME, et al. Basement membrane assembly and differentiation of cultured corneal cells: importance of culture environment and endothelial cell interaction. Exp Cell Res 1994;214:621-33.
24. Engelmann K, Friedl P. Optimization of culture conditions for human corneal endothelial cells. In Vitro Cell Dev Biol 1989;25:1065-72.
25. Bednarz J, Teifel M, Friedl P, et al. Immortalization of human corneal endothelial cells using electroporafion protocol optimized for human corneal endothelial and human retinal pigment epithelial cells. Acta Ophthalmol Scand 2000;78:130-36.
26. Fujita T, Tanaka K, Tokunaga J. Zellen und Gewebe: Ein REM-Atlas für Mediziner und Biologen. Stuttgart: Gustav Fischer Verlag, 1986.
27. Aboalchamat B, Engelmann K, Böhnke M, et al. Morphological and functional analysis of immortalized human corneal endothelial cells after transplantation. Exp Eye Res 1999;69:547-53.
28. Kahn CR, Young E, Lee ICH, et al. Human corneal epithelial primary cultures and cell lines with extended life span: in vitro model for ocular studies. Invest Ophthalmol Vis Sci 1993;34:3429-41.
29. Araki-Sasaki K, Ohashi Y, Sasabe T, et al. An SV40-immortalized human corneal epithelial cell line and its characterization. Invest Ophthalmol Vis Sci 1995;36:614-21.
30. Germain L, Carrier P, Auger FA, et al. Can we produce a human corneal equivalent by tissue engineering? Prog Retin Eye Res 2000;19:497-527.
31. Lehr CM. Cell culture models of biological barriers: in vitro test systems for drug absorption and delivery. London: Taylor & Francis, 2002.
32. Minami Y, Sugihara H, Oono S. Reconstruction of cornea in three-dimensional collagen gel matrix culture. Invest Ophthalmol Vis Sci 1993;34:2316-24.
33. Parnigotto PP, Bassani V, Montesi F, et al. Bovine corneal stroma and epithelium reconstructed in vitro: characterisation and response to surfactants. Eye 1998;12:304-10.
34. Schneider AI, Maier-Reif K, Graeve T. Constructing an in vitro cornea from cultures of the three specific corneal cell types. In Vitro Cell Dev Biol Anim 1999;35:515-26.
35. Griffith M, Osborne R, Munger R, et al. Functional human corneal equivalents constructed from cell lines. Science 1999;286:2169-72.
36. Goskonda VR, Hill RA, Khan MA, et al. Permeability of chemical delivery systems across rabbit corneal (SIRC) cell line and isolated corneas: a comparative study. Pharm Dev Technol 2000;5:409-16.
37. Nishida T. Cornea. In: Krachmer JH, ed. Cornea. St Louis, MO: Mosby, 1997:3-60.
38. Ehlers N. Morphology and histochemistry of the corneal epithelium of mammals. Acta Anat 1970;75:161-98.
39. Pond WG, Houpt KA. The biology of the pig. Ithaca and London: Cornell University Press, 1978.