Improved reactivation of immobilized-stabilized lipase from Thermomyces lanuginosus by its coating with highly hydrophilic polymers

Journal of Biotechnology

Volumn 144, Issue 2, 2009, Pages 113-119

  Rodrigues, Rafael C ^a   Bolivar, Juan M ^a   Volpato, Giandra ^a   Filice, Marco ^a   Godoy, Cesar ^a   Fernandez Lafuente, Roberto ^a   Guisan, Jose M ^a  

^a INSTITUTO DE CATÁLISIS Y PETROLEOQUÍMICA   (Spain)

Author keywords

Chemical modification; Enzyme reactivation; Enzyme stabilization; Hydrophilic polymers; Lipase; Solid phase

Indexed keywords

ACTIVE SITE; AQUEOUS MEDIUM; ENZYMATIC ACTIVITIES; ENZYME REACTIVATION; ENZYME STABILIZATION; HIGH TEMPERATURE; HYDROPHILIC AND HYDROPHOBIC; HYDROPHILIC POLYMERS; INERT POLYMERS; INITIAL ACTIVITY; MODIFIED ENZYMES; SOLID PHASE; THERMOMYCES LANUGINOSUS;

CHEMICAL MODIFICATION; ENZYMES; GLUCOSE; HYDROPHILICITY; ORGANIC SOLVENTS; PLASTIC COATINGS; POLYMERS; SOAPS (DETERGENTS); STABILIZATION;

ENZYME ACTIVITY;

DEXTRAN; FORMALDEHYDE; GLYCINE; GUANIDINE; ORGANIC SOLVENT; POLYMER; TRIACYLGLYCEROL LIPASE;

ARTICLE; BIOCATALYST; CHEMICAL MODIFICATION; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME IMMOBILIZATION; ENZYME INACTIVATION; ENZYME REACTIVATION; ENZYME STABILITY; FUNGUS; HIGH TEMPERATURE; HYDROPHILICITY; MATERIAL COATING; NONHUMAN; PH; PRIORITY JOURNAL; THERMOMYCES LANUGINOSUS;

AMINES; ASCOMYCOTA; COLORIMETRY; ENZYME ACTIVATION; ENZYME STABILITY; ENZYMES, IMMOBILIZED; GLYOXYLATES; GUANIDINE; HOT TEMPERATURE; LIPASE; POLYMERS; SEPHAROSE; SOLVENTS; TIME FACTORS; TITRIMETRY;

THERMOMYCES LANUGINOSUS;

EID: 70350223483     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2009.09.002     Document Type: Article

References (36)

1. Bornscheuer U.T., and Kazlauskas R.J. Hydrolases in Organic Synthesis: Regio- and Stereoselective Biotransformations (2006), Wiley-VCH, Weinheim
2. Brzozowski A.M., Derewenda U., Derewenda Z.S., Dodson G.G., Lawson D.M., Turkenburg J.P., Bjorkling F., Huge-Jensen B., Patkar S.A., and Thim L. A model for interfacial activation in lipases from the structure of a fungal lipase-inhibitor complex. Nature 351 (1991) 491-494
3. Cao L. Immobilised enzymes: science or art?. Curr. Opin. Chem. Biol. 9 (2005) 217-226
4. Daniel R.M., Dines M., and Petach H.H. The denaturation and degradation of stable enzymes at high temperatures. Biochem. J. 317 (1996) 1-11
5. Derewenda U., Swenson L., Green R., Wei Y., Yamaguchi S., Joerger R., Haas M.J., and Derewenda Z.S. Current progress in crystallographic studies of new lipases from filamentous fungi. Protein Eng. 7 (1994) 551-557
6. Fernandez-Lafuente R., Rosell C.M., Alvaro G., and Guisan J.M. Additional stabilization of penicillin G acylase-agarose derivatives by controlled chemical modification with formaldehyde. Enzyme Microb. Technol. 14 (1992) 489-495
7. Fernandez-Lafuente R., Armisen P., Sabuquillo P., Fernandez-Lorente G., and Guisan J.M. Immobilization of lipases by selective adsorption on hydrophobic supports. Chem. Phys. Lipids 93 (1998) 185-197
8. Fernandez-Lafuente R., Rosell C.M., Caanan-Haden L., Rodes L., and Guisan J.M. Facile synthesis of artificial enzyme nano-environments via solid-phase chemistry of immobilized derivatives: dramatic stabilization of penicillin acylase versus organic solvents. Enzyme Microb. Technol. 24 (1999) 96-103
9. Fernández-Lafuente R., Rosell C.M., and Guisán J.M. The presence of methanol exerts a strong and complex modulation of the synthesis of different antibiotics by immobilized Penicillin G acylase. Enzyme Microb. Technol. 23 (1998) 305-310
10. Fernández-Lorente G., Palomo J.M., Mateo C., Munilla R., Ortiz C., Cabrera Z., Guisán J.M., and Fernández-Lafuente R. Glutaraldehyde cross-linking of lipases adsorbed on aminated supports in the presence of detergents leads to improved performance. Biomacromolecules 7 (2006) 2610-2615
11. Fuentes M., Segura R.L., Abian O., Betancor L., Hidalgo A., Mateo C., Fernandez-Lafuente R., and Guisan J.M. Determination of protein-protein interactions through aldehyde-dextran intermolecular cross-linking. Proteomics 4 (2004) 2602-2607
12. Fuentes M., Mateo C., García L., Tercero J.C., Guisán J.M., and Fernández-Lafuente R. Directed covalent immobilization of aminated DNA probes on aminated plates. Biomacromolecules 5 (2004) 883-888
13. Guncheva M., Zhiryakova D., Radchenkova N., and Kambourova M. Effect of nonionic detergents on the activity of a thermostable lipase from Bacillus stearothermophilus MC7. J. Mol. Catal. B: Enzym. 49 (2007) 88-91
14. Hoare D.G., Olson A., and Koshland Jr. D.E. The reaction of hydroxamic acids with water-soluble carbodiimides. A Lossen rearrangement. J. Am. Chem. Soc. 90 (1968) 1638-1643
15. Ikegaya K. Kinetic analysis about the effects of neutral salts on the thermal stability of yeast alcohol dehydrogenase. J. Biochem. 137 (2005) 349-354
16. Iyer P.V., and Ananthanarayan L. Enzyme stability and stabilization: aqueous and non-aqueous environment. Process Biochem. 43 (2008) 1019-1032
17. Kim P.Y., Pollard D.J., and Woodley J.M. Substrate supply for effective biocatalysis. Biotechnol. Prog. 23 (2007) 74-82
18. Mateo C., Abian O., Ernedo M.B., Cuenca E., Fuentes M., Fernandez-Lorente G., Palomo J.M., Grazu V., Pessela B.C.C., Giacomini C., Irazoqui G., Villarino A., Ovsejevi K., Batista-Viera F., Fernandez-Lafuente R., and Guisan J.M. Some special features of glyoxyl supports to immobilize proteins. Enzyme Microb. Technol. 37 (2005) 456-462
19. Mateo C., Palomo J.M., Fernandez-Lorente G., Guisan J.M., and Fernandez-Lafuente R. Improvement of enzyme activity, stability and selectivity via immobilization techniques. Enzyme Microb. Technol. 40 (2007) 1451-1463
20. Miled N., Beisson F., De Caro J., De Caro A., Arondel V., and Verger R. Interfacial catalysis by lipases. J. Mol. Catal. B: Enzym. 11 (2001) 165-171
21. Mogensen J.E., Sehgal P., and Otzen D.E. Activation, inhibition, and destabilization of Thermomyces lanuginosus lipase by detergents. Biochemistry 44 (2005) 1719-1730
22. Palomo J.M., Fuentes M., Fernandez-Lorente G., Mateo C., Guisan J.M., and Fernandez-Lafuente R. General trend of lipase to self-assemble giving bimolecular aggregates greatly modifies the enzyme functionality. Biomacromolecules 4 (2003) 1-6
23. Polizzi K.M., Bommarius A.S., Broering J.M., and Chaparro-Riggers J.F. Stability of biocatalysts. Curr. Opin. Chem. Biol. 11 (2007) 220-225
24. Rehaber V., and Jaenicke R. Stability and reconstitution of d-glyceraldehyde-3-phosphate dehydrogenase from the hyperthermophilic eubacterium Thermotoga maritima. J. Biol. Chem. 267 (1992) 10999-11006
25. Rodrigues R.C., Godoy C.A., Volpato G., Ayub M.A.Z., Fernandez-Lafuente R., and Guisan J.M. Immobilization-stabilization of the lipase from Thermomyces lanuginosus: critical role of chemical amination. Process Biochem. 44 (2009) 963-968
26. Costa Rodrigues R., Volpato G., Wada K., and Záchia Ayub M.A. Improved enzyme stability in lipase-catalyzed synthesis of fatty acid ethyl ester from soybean oil. Appl. Biochem. Biotechnol. 152 (2009) 394-404
27. Rodrigues R.C., Bolivar J.M., Palau-Ors A., Volpato G., Ayub M.A.Z., Fernandez-Lafuente R., and Guisan J.M. Positive effects of the multipoint covalent immobilization in the reactivation of partially inactivated derivatives of lipase from Thermomyces lanuginosus. Enzyme Microb. Technol. 44 (2009) 386-393
28. Rodrigues R.C., Godoy C.A., Filice M., Bolivar J.M., Palau-Ors A., Garcia-Vargas J.M., Romero O., Wilson L., Ayub M.A.Z., Fernandez-Lafuente R., and Guisan J.M. Reactivation of covalently immobilized lipase from Thermomyces lanuginosus. Process Biochem. 44 (2009) 641-646
29. Romero O., Vergara J., Fernández-Lafuente R., Guisán J.M., Illanes A., and Wilson L. Simple strategy of reactivation of a partially inactivated penicillin G acylase biocatalyst in organic solvent and its impact on the synthesis of β-lactam antibiotics. Biotechnol. Bioeng. 103 (2009) 472-479
30. Rosell C.M., Terreni M., Fernandez-Lafuente R., and Guisan J.M. A criterion for the selection of monophasic solvents for enzymatic synthesis. Enzyme Microb. Technol. 23 (1998) 64-69
31. Sarmento A.C., Oliveira C.S., Pereira A., Esteves V.I., Moir A.J.G., Saraiva J., Pires E., and Barros M. Unfolding of cardosin A in organic solvents and detection of intermediaries. J. Mol. Catal. B: Enzym. 57 (2009) 115-122
32. Sheldon R.A. Enzyme immobilization: the quest for optimum performance. Adv. Synth. Catal. 349 (2007) 1289-1307
33. Snyder S.L., and Sobocinski P.Z. An improved 2,4,6 trinitrobenzenesulfonic acid method for the determination of amines. Anal. Biochem. 64 (1975) 284-288
34. Turan S., Karabulut I., and Vural H. Effects of reaction parameters on the incorporation of caprylic acid into soybean oil for production of structured lipids. J. Food Lipids 13 (2006) 306-317
35. Yeates C.A., Krieg H.M., and Breytenbach J.C. Hydroxypropyl-β-cyclodextrin induced complexation for the biocatalytic resolution of a poorly soluble epoxide. Enzyme Microb. Technol. 40 (2007) 228-235
36. Zhi W., Landry S.J., Gierasch L.M., and Srere P.A. Renaturation of citrate synthase: influence of denaturant and folding assistants. Protein Sci. 1 (1992) 522-529