Ovine Ruminal Microbes Are Capable of Biotransforming Hexahydro-1,3,5-Trinitro-1,3,5-Triazine (RDX)

Microbial Ecology

Volumn 62, Issue 2, 2011, Pages 274-286

  Eaton, H L ^a   De Lorme, M ^b   Chaney, R L ^c   Craig, A M ^a  

^a OREGON STATE UNIVERSITY   (United States)

^b Oklahoma State University   (United States)

^c Environmental Management and Byproduct Utilization Laboratory   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARCHAEAL DNA; BACTERIAL DNA; CYCLONITE; NITROGEN; TRIAZINE DERIVATIVE;

ANIMAL; ARCHAEBACTERIUM; ARTICLE; BACTERIUM; BIOREMEDIATION; CHEMISTRY; CULTURE MEDIUM; DENATURING GRADIENT GEL ELECTROPHORESIS; GENETIC VARIABILITY; GENETICS; GROWTH, DEVELOPMENT AND AGING; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ISOLATION AND PURIFICATION; MALE; METABOLISM; MICROBIAL CONSORTIUM; MICROBIOLOGY; PHYLOGENY; RNA GENE; RUMINANT STOMACH; SEQUENCE HOMOLOGY; SHEEP; TIME;

ANIMALS; ARCHAEA; BACTERIA; BIODEGRADATION, ENVIRONMENTAL; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CULTURE MEDIA; DENATURING GRADIENT GEL ELECTROPHORESIS; DNA, ARCHAEAL; DNA, BACTERIAL; GENES, RRNA; GENETIC VARIATION; MALE; MICROBIAL CONSORTIA; NITROGEN; PHYLOGENY; RUMEN; SEQUENCE HOMOLOGY, NUCLEIC ACID; SHEEP; TIME FACTORS; TRIAZINES;

ACTINOBACTERIA; ARCHAEA; BACTERIA (MICROORGANISMS); BOVIDAE; BOVINAE; CLOSTRIDIA; EURYARCHAEOTA; FIRMICUTES; OVIS; SPORANAEROBACTER ACETIGENES;

EID: 80051666678     PISSN: 00953628     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00248-011-9809-8     Document Type: Article

References (54)

1. Adrian NR, Arnett CM, Hickey RF (2003) Stimulating the anaerobic biodegradation of explosives by the addition of hydrogen or electron donors that produce hydrogen. Water Res 37: 3499-3507.
2. Agency for Toxic substances and Disease Registry. Toxicological profile for RDX. Atlanta, GA: U. S. Department of Health and Human Services, Public Health Service, 1995.
3. Alfreider A, Vogt C (2007) Bacterial diversity and aerobic biodegradation potential in a BTEX-contaminated aquifer. Water Air Soil Pollut 183: 415-426.
4. Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25: 3389-3402.
5. An D, Cai S, Dong X (2006) Actinomyces ruminicola sp. nov., isolated from cattle rumen. Int J Syst Evol Microbiol 56: 2043-2048.
6. Benson DA, Karsch-Mizrachi I, Lipman DJ, Ostell J, Wheeler DL (2005) GenBank. Nucleic Acids Res 34: D16-D20.
7. Bhadra R, Wayment DG, Williams RK, Barman SN, Stone MB, Hughes JB, Shanks JV (2001) Studies on plant-mediated fate of the explosives RDX and HMX. Chemosphere 44: 1259-1264.
8. Burken JG. Phytoremediation/wetlands treatment at the Iowa Army Ammunition Plant. Columbus, OH: McGraw-Hill Companies, 2001. http://www. mhhe. com/biosci/pae/environmentalscience/casestudies/case12. mhtml.
9. Chaucheyras F, Fonty G et al (1995) In vitro H2 utilization by a ruminal acetogenic bacterium cultivated alone or in combination with an Archaea methanogen is stimulated by a probiotic strain of Saccharomyces cerevisiae. Appl Environ Microbiol 6(1).
10. Coppotelli BM, Ibarrolaza A, Del Panno MT, Morelli IS (2008) Effects of the inoculant strain Sphingomonas paucimobilis 20006FA on soil bacterial community and biodegradation in phenanthrene-contaminated soil. Microb Ecol 55: 173-183.
11. Daniels JI, Knezovich JP (1994) Human health risks from TNT, RDX, and HMX in environmental media and consideration of the U. S. Regulatory Environment. In: International Symposium on the Rehabilitation of Former Military Sites and Demilitarization of Explosive Ordnance. Kirchberg, Luxembourg: Proceedings Demil '94.
12. De Lorme M, Craig AM (2008) Biotransformation of 2, 4, 6-trinitrotoluene by pure culture ruminal bacteria. Curr Microbiol 58: 81-86.
13. Delong EF (1992) Archaea in coastal marine environments. Proceedings of the National Academy of Sciences 89: 5685-5689.
14. Dworkin M, Falkow S, Rosenburg E, Schleifer KH, Stackebrandt E (2006) The Prokaryotes: Ecophysiology and Biochemistry. New York, NY, Springer.
15. Engebrecht J, Brent R, Kaderbhai M (2001) Minipreps of plasmid DNA. Current protocols in molecular biology. F. M. Ausubel, R. Brent, R. E. Kingston et al., John Wiley & Sons, Inc. doi: 10. 1002/0471142727. mb0106s15.
16. Felenstein J (2004) PHYLIP (Phylogeny Inference Package) version 3. 6. Seattle, WA: J. Felenstein, Department of Genome Sciences, University of Washington.
17. Fleischmann TJ, Walker KC, Spain JC, Hughes JB, Morrie Craig A (2004) Anaerobic transformation of 2, 4, 6-TNT by bovine ruminal microbes. Biochem Biophys Res Commun 314: 957-963.
18. Fournier D, Trott S, Hawari J, Spain J (2005) Metabolism of the aliphatic nitramine 4-nitro-2, 4-diazabutanal by Methylobacterium sp. strain JS178. Appl Environ Microbiol 71: 4199-4202.
19. Greening R, Leedle J (1989) Enrichment and isolation of Acetitomaculum ruminis, gen. nov., sp. nov.: acetogenic bacteria from the bovine rumen. Archives of Microbiology 151(5): 399-406.
20. Hawari J, Beaudet S, Halasz A, Thiboutot S, Ampleman G (2000) Microbial degradation of explosives: biotransformation versus mineralization. Appl Microbiol Biotechnol 54: 605-618.
21. Hawari J, Halasz A, Sheremata T, Beaudet S, Groom C, Paquet L, Rhofir C, Ampleman G, Thiboutot S (2000) Characterization of metabolites during biodegradation of hexahydro-1, 3, 5-trinitro-1, 3, 5-triazine (RDX) with municipal anaerobic sludge. Appl Environ Microbiol 66: 2652-2657.
22. Hernandez-Eugenio G, Fardeau ML, Cayol JL, Patel BKC, Thomas P, Macarie H, Garcia JL, Ollivier B (2002) Sporanaerobacter acetigenes gen. nov., sp. nov., a novel acetogenic, facultatively sulfur-reducing bacterium. Int J Syst Evol Microbiol 52: 1217-1223.
23. Hobson PN, Stewart CS (1997) The rumen microbial ecosystem. Blackie Academic and Professional, New York, NY, p 709.
24. Hwang K, Shin SG, Kim J, Hwang S (2008) Methanogenic profiles by denaturing gradient gel electrophoresis using order-specific primers in anaerobic sludge digestion. Appl Microbiol Biotechnol 80: 269-276.
25. Joblin KN (1999) Ruminal acetogens and their potential to lower ruminant methane emissions. Aust J Agric Res 50: 1307-1313.
26. Krumholz LR, Bryant MP (1985) Clostridium pfennigii sp. nov. Uses Methoxyl Groups of Monobenzenoids and Produces Butyrate. Int J Syst Bacteriol 35(4): 454-456.
27. Krumholz LR, Bryant MP (1986) Syntrophococcus sucromutans sp. nov. gen. nov. uses carbohydrates as electron donors and formate, methoxymonobenzenoids or Methanobrevibacter as electron acceptor systems. Archives of microbiology 143(4): 313-318.
28. Liu C, Finegold SM, Song Y, Lawson PA (2008) Reclassification of Clostridium coccoides, Ruminococcus hansenii, Ruminococcus hydrogenotrophicus, Ruminococcus luti, Ruminococcus productus and Ruminococcus schinkii as Blautia coccoides gen. nov., comb. nov., Blautia hansenii comb. nov., Blautia hydrogenotrophica comb. nov., Blautia luti comb. nov., Blautia producta comb. nov., Blautia schinkii comb. nov. and description of Blautia wexlerae sp. nov., isolated from human faeces. Int J Syst Evol Microbiol 58: 1896-1902.
29. Long R, Lappin-Scott H, Stevens J (2009) Enrichment and identification of polycyclic aromatic compound-degrading bacteria enriched from sediment samples. Biodegradation 20: 521-531.
30. Ozutsumi Y, Tajima K, Takenaka A, Itabashi H (2005) The effect of protozoa on the composition of rumen bacteria in cattle using 16S rRNA gene clone libraries. Bioscience, Biotechnol and Biochem 69: 499-506.
31. Pearson WR (1991) Searching protein sequence libraries: comparison of the sensitivity and selectivity of the Smith-Waterman and FASTA algorithms. Genomics 11: 635-650.
32. Rattray RM, Craig AM (2007) Molecular characterization of sheep ruminal enrichments that detoxify pyrrolizidine alkaloids by denaturing gradient gel electrophoresis and cloning. Microb Ecol 54: 264-275.
33. Rieu-Lesme F, Fonty G et al (1995) Isolation and characterization of a new hydrogen-utilizing bacterium from the rumen. FEMS Microbiology Letters 125(1): 77-82.
34. Rieu-Lesme F, D C et al (1996a) Acetogenic coccoid spore-forming bacteria isolated from the rumen. Research in Microbiology 147(9): 753-764.
35. Rieu-Lesme F, Morvan B et al (1996b) A new H2 / CO2-using acetogenic bacterium from the rumen: description of Ruminococcus schinkii sp. nov. FEMS Microbiology Letters 140(2-3): 281-286.
36. Rieu-Lesme F, Dauga C et al (1998) Isolation from the rumen of a new acetogenic bacterium phylogenetically closely related to Clostridium difficile. Anaerobe 4(2): 89-94.
37. Roling WFM, Couto de Brito IR, Swannell RPJ, Head IM (2004) Response of archaeal communities in beach sediments to spilled oil and bioremediation. Appl Environ Microbiol 70: 2614-2620.
38. Rylott EL, Jackson RG, Edwards J, Womack GL, Seth-Smith HMB, Rathbone DA, Strand SE, Bruce NC (2006) An explosive-degrading cytochrome p450 activity and its targeted application for the phytoremediation of RDX. Nat Biotechnol 24: 216-219.
39. Saia F, Damianovic M, Cattony E, Brucha G, Foresti E, Vazoller R (2007) Anaerobic biodegradation of pentachlorophenol in a fixed-film reactor inoculated with polluted sediment from Santos-São Vicente Estuary. Brazil Appl Microbiol Biotechnol 75: 665-672.
40. Sejrsen K, Hvelplund T, Nielsen MO (2006) Ruminant physiology: digestion, metabolism and impact of nutrition on gene expression, immunology and stress. Wageningen Academic Publishers, Wageningen, The Netherlands, p 600.
41. Smith DJ, Craig AM, Duringer JM, Chaney RL (2008) Absorption, tissue distribution, and elimination of residues after 2, 4, 6-trinitro[14C]toluene administration to sheep. Environ Sci Technol 42: 2563-2569.
42. Smith TF, Waterman MS (1981) Identification of common molecular subsequences. J Mol Biol 147: 195-197.
43. Sunahara GI, Guilherme L, Kuperman RG, Hawari J (2009) Ecotoxicology of explosives. CRC Press, Boca Raton, FL, p 325.
44. Tajima K, Aminov RI, Nagamine T, Matsui H, Nakamura M, Benno Y (2001) Diet-dependent shifts in the bacterial population of the rumen revealed with real-time PCR. Appl Environ Microbiol 67: 2766-2774.
45. Tajima K, Aminov RI, Nagamine T, Ogata K, Nakamura M, Matsui H, Benno Y (1999) Rumen bacterial diversity as determined by sequence analysis of 16S rDNA libraries. FEMS Microbiol Ecol 29: 159-169.
46. Talley JW, Sleeper PM (1997) Roadblocks to the implementation of biotreatment strategies. Annals New York Academy of Sciences 829: 16-29.
47. Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22: 4673-4680.
48. U. S. Environmental Protection Agency (2006) Method 8330B (SW-846): Nitroaromatics, Nitramines, and Nitrate Esters by High Performance Liquid Chromatography (HPLC). Washington, DC: EPA, p. 60.
49. U. S. Environmental Protection Agency (2009) Hexahydro-1, 3, 5-trinitro-1, 3, 5-triazine (RDX). Washington, DC: Environmental Protection Agency.
50. Ventura M, Canchaya C, Tauch A, Chandra G, Fitzgerald GF, Chater KF, van Sinderen D (2007) Genomics of Actinobacteria: tracing the evolutionary history of an ancient phylum. Microbiol Mol Biol Rev 71: 495-548.
51. Vila M, Lorber-Pascal S, Laurent F (2007) Fate of RDX and TNT in agronomic plants. Environ Pollut 148: 148-154.
52. Vila M, Mehier S, Lorber-Pascal S, Laurent F (2007) Phytotoxicity to and uptake of RDX by rice. Environ Pollut 145: 813-817.
53. Zhao JS, Paquet L, Halasz A, Hawari J (2003) Metabolism of hexahydro-1, 3, 5-trinitro-1, 3, 5-triazine through initial reduction to hexahydro-1-nitroso-3, 5-dinitro-1, 3, 5-triazine followed by denitration in Clostridium bifermentans HAW-1. Appl Microbiol Biotechnol 63: 187-193.
54. Zrafi-Nouira I, Guermazi S, Chouari R, Safi N, Pelletier E, Bakhrouf A, Saidane-Mosbahi D, Sghir A (2009) Molecular diversity analysis and bacterial population dynamics of an adapted seawater microbiota during the degradation of Tunisian zarzatine oil. Biodegradation 20: 467-486.