Bacterial diversity and aerobic biodegradation potential in a BTEX-contaminated aquifer

Water, Air, and Soil Pollution

Volumn 183, Issue 1-4, 2007, Pages 415-426

  Alfreider, Albin ^a,b   Vogt, Carsten ^a  

^a HELMHOLTZ CENTRE FOR ENVIRONMENTAL RESEARCH UFZ   (Germany)

^b UNIVERSITY OF INNSBRUCK   (Austria)

Author keywords

Benzene; Biodegradation; BTEX; Groundwater bacterial community; Polyphasic approach

Indexed keywords

BENZENE; BIODEGRADATION; CONTAMINATION; GROUNDWATER POLLUTION; OLIGOMERS; RNA;

FLUORESCENT IN SITU HYBRIDIZATION (FISH); GROUNDWATER BACTERIAL COMMUNITY; POLYPHASIC APPROACH;

WATER ANALYSIS;

AROMATIC COMPOUND; BENZENE; CATECHOL 2,3 DIOXYGENASE; DNA 16S; GROUND WATER; OLIGONUCLEOTIDE; RIBOSOME RNA; TOLUENE;

BENZENE; BIODEGRADATION; CONTAMINATION; GROUNDWATER POLLUTION; OLIGOMERS; RNA; WATER ANALYSIS;

AQUIFER POLLUTION; BACTERIUM; BENZENE; BIODEGRADATION; BTEX; COMMUNITY STRUCTURE; DNA; GENETIC ANALYSIS; PLUME; TOLUENE; XYLENE;

AEROBIC BACTERIUM; AQUIFER; ARTICLE; BACTERIAL FLORA; BIODEGRADATION; BIODIVERSITY; CONTROLLED STUDY; DNA SEQUENCE; FIRMICUTES; FLUORESCENCE IN SITU HYBRIDIZATION; GENE TRANSFER; GEOBACTER; MOLECULAR PROBE; NONHUMAN; NUCLEOTIDE SEQUENCE; PLUME DISPERSION; POLLUTION; PROTEOBACTERIA; SAMPLING; SPOROGENESIS; SULFATE REDUCING BACTERIUM;

BACTERIA (MICROORGANISMS); BETAPROTEOBACTERIA; FIRMICUTES; GEOBACTER; PROTEOBACTERIA;

EID: 34249791212     PISSN: 00496979     EISSN: 15732932     Source Type: Journal    
DOI: 10.1007/s11270-007-9390-4     Document Type: Article

References (45)

1. Alfreider, A., Pernthaler, J., Amann, R., Sattler, B., Glöckner, F. O., Wille, A., et al. (1996). Community analysis of the bacterial assemblages in the winter cover and pelagic layers of a high mountain lake by in situ hybridization. Applied and Environmental Microbiology, 62, 2138-2144.
2. Alfreider, A., Vogt, C., & Babel, W. (2002). Microbial diversity in an in situ reactor system treating monochlorobenzene contaminated groundwater as revealed by 16S ribosomal DNA analysis. Systematic and Applied Microbiology, 25, 232-240.
3. Altschul, S. F., Gish, W., Miller, W., Myers, E. W., & Lipman, D. J. (1990). Basic local alignment search tool. Journal of Molecular Biology, 215, 403-410.
4. Aronson, D., & Howard, P. H. (1997). Anaerobic degradation of organic chemicals in groundwater: A summary of field and laboratory studies. Final Report. New York: Environmental Science Center, Syracus Research Cooperation.
5. Brinkhoff, T., Santegoeds, C. M., Sahm, K., Kuever, J., & Muyzer, G. (1998). A polyphasic approach to study the diversity and vertical distribution of sulfur-oxidizing thiomicrospira species in coastal sediments of the german wadden Sea. Applied and Environmental Microbiology, 64, 4650-4657.
6. Brune, A., Ludwig, W., & Schink, B. (2002). Propionivibrio limicola sp. nov., a fermentative bacterium specialized in the degradation of hydroaromatic compounds, reclassification of Propionibacter pelophilus as Propionivibrio pelophilus comb. nov and amended description of the genus Propionivibrio. International Journal of Systematic and Evolutionary Microbiology, 52, 441-444.
7. Cerniglia, C. E. (1984). Microbial metabolism of polycyclic aromatic hydrocarbons. Advances in Applied Microbiology, 30, 31-71.
8. Christensen, H., Hansen, M., & Sorensen, J. (1999). Counting and size classification of active soil bacteria by fluorescence in situ hybridization with an rRNA oligonucleotide probe. Applied and Environmental Microbiology, 65, 1753-1761.
9. Cole, J. R., Chai, B., Farris, R. J., Wang, Q., Kulam, S. A., McGarrell, D. M., et al. (2005). The Ribosomal Database Project (RDP-II): Sequences and tools for high-throughput rRNA analysis. Nucleic Acids Research, 33, D294-D296.
10. Dojka, M. A., Hugenholtz, P., Haack, S. K., & Pace, N. R. (1998). Microbial diversity in a hydrocarbon- and chlorinated-solvent-contaminated aquifer undergoing intrinsic bioremediation. Applied and Environmental Microbiology, 64, 3869-3877.
11. Ellis, R. J., Morgan, P., Weightman, A. J., & Fry, J. C. (2003). Cultivation-dependent and -independent approaches for determining bacterial diversity in heavy-metal-contaminated soil. Applied and Environmental Microbiology, 69, 3223-3330.
12. Fahy, A., McGenity, T. J., Timmis, K. N., & Ball, A. S. (2006). Heterogeneous aerobic benzene-degrading communities in oxygen-depleted groundwaters. FEMS Microbiology Ecology, 58, 260-270.
13. Feris, K. P., Hristova, K., Gebreyesus, B., Mackay, D., & Scow, K. M. (2004). A shallow BTEX and MTBE contaminated aquifer supports a diverse microbial community. Microbial Ecology, 48, 589-600.
14. Geyer, R., Peacock, A. D., Miltner, A., Richnow, H. H., White, D. C., Sublette, K. L., et al. (2005). In situ assessment of biodegradation potential using biotraps amended with C-13-labeled benzene or toluene. Environmental Science & Technology, 39, 4983-4989.
15. Glöckner, F. O., Amann, R., Alfreider, A., Pernthaler, J., Psenner, R., Trebesius, K. H., et al. (1996). An in situ hybridization protocol for detection and identification of planktonic bacteria. Systematic and Applied Microbiology, 19, 403-406.
16. Grabowski, A., Nercessian, O., Fayolle, F., Blanchet, D., & Jeanthon, C. (2005). Microbial diversity in production waters of a low-temperature biodegraded oil reservoir. FEMS Microbiology Ecology, 54, 427-443.
17. Hugenholtz, P., Pitulle, C., Hershberger, K. L., & Pace, N. R. (1998). Novel division level bacterial diversity in a yellowstone hot spring. Journal of Bacteriology, 180, 366-376.
18. Hughes, J. B., Hellmann, J. J., Ricketts, T. H., & Bohannan, B. J. M. (2001). Counting the uncountable: Statistical approaches to estimating microbial diversity. Applied and Environmental Microbiology, 67, 4399-4406.
19. Johnson, S. J., Woolhouse, K. J., Prommer, H., Barry, D. A., & Christofi, N. (2003). Contribution of anaerobic microbial activity to natural attenuation of benzene in groundwater. Engineering Geology, 70, 343-349.
20. Kalmbach, S., Manz, W., Wecke, J., & Szewzyk, U. (1999). Aquabacterium gen. nov., with description of Aquabacterium citratiphilum sp. nov., Aquabacterium parvum sp. nov. and Aquabacterium commune sp. nov., three in situ dominant bacterial species from the Berlin drinking water system. International Journal of Systematic Bacteriology, 49, 769-777.
21. Kemp, P. F., & Aller, J. Y. (2004). Estimating prokaryotic diversity: When are 16S rDNA libraries large enough? Limnology and Oceanography: Methods, 2, 114-125.
22. Kowalchuk, G. A., de Bruijn, F. J., Head, I. M., Akkermans, A. D., & van Elsas, J. D. (Eds.) (2004). Molecular microbial ecology manual (2nd ed., vols. 1 and 2). Dordrecht, The Netherlands: Kluwer.
23. Kukor, J. J., & Olsen, R. H. (1996). Catechol 2,3-dioxygenases functional in oxygen-limited (hypoxic) environments. Applied and Environmental Microbiology, 62, 1728-1740.
24. Lane, D. J. (1991). 16S/23S sequencing. In Nucleic acid techniques in bacterial systematics (pp 148-163). New York: Wiley.
25. Ludwig, W., Strunk, O., Westram, R., Richter, L., Meier, H., Yadhukumar, et al. (2004). ARB: A software environment for sequence data. Nucleic Acids Research, 32, 1363-1371.
26. Maidak, B. L., Cole, J. R., Lilburn, T. G., Parker, C. T. Jr., Saxman, P. R., Farris, R. J., et al. (2001). The RDP-II (Ribosomal Database Project). Nucleic Acids Research, 29, 173-174.
27. Meckenstock, R. U., Morasch, B., Griebler, C., & Richnow, H. H. (2004). Stable isotope fractionation analysis as a tool to monitor biodegradation in contaminated aquifers. Journal of Contaminant Hydrology, 75, 215-255.
28. Olsen, R. H., Mikesell, M. D., Kukor, J. J., & Byrne, A. M. (1995). Physiological attributes of microbial BTEX degradation in oxygen-limited environments. Environmental Health Perspectives, 103, 49-51.
29. Reasoner, D. J., & Geldreich, E. E. (1985). A new medium for the enumaration and subculture of bacteria from potable water. Applied and Environmental Microbiology, 49, 1-7.
30. Robertson, W. J., Franzmann, P. D., & Mee, B. J. (2000). Spore-forming, Desulfosporosinus-like sulphate-reducing bacteria from a shallow aquifer contaminated with gasoline. Journal of Applied Microbiology, 88, 248-259.
31. Rooney-Varga, J. N., Anderson, R. T., Fraga, J. L., Ringelberg, D., & Lovley, D. R. (1999). Microbial communities associated with anaerobic benzene degradation in a petroleum-contaminated aquifer. Applied and Environmental Microbiology, 65, 3056-3063.
32. Schippers, A., Neretin, L. N., Kallmeyer, J., Ferdelman, T. G., Cragg, B. A., Parkes, R. J., et al. (2005). Prokaryotic cells of the deep sub-seafloor biosphere identified as living bacteria. Nature, 43, 861-864.
33. Schirmer, M., Dahmke, A., Dietrich, P., Dietze, M., Gödeke, S., Richnow, H. H., et al. (2006). Natural attenuation research at the contaminated megasite Zeitz. Journal of Hydrology, 328, 393-407.
34. Schirmer, M., Jones, I., Teutsch, G., & Lerner, D. N. (1995). Development and testing of multiport sock samplers for groundwater. Journal of Hydrology, 171, 239-257.
35. Sei, K., Asano, K., Tateishi, N., Mori, K., Ike, M., & Fujita, M. (1999). Design of PCR primers and gene probes for the general detection of bacterial populations capable of degrading aromatic compounds via catechol cleavage pathways. Journal of Bioscience and Bioengineering, 88, 542-550.
36. Stelzer, N., Büning, C., Pfeifer, F., Dohrmann, A. B., Tebbe, C., Nijenhuis, I., et al. (2006). In situ microcosms to evaluate natural attenuation potentials in contaminated aquifers. Organic Geochemistry, 37, 1394-1410.
37. Straub, K. L., Schonhuber, W. A., Buchholz-Cleven, B. E. E., & Schink, B. (2004). Diversity of ferrous iron-oxidizing, nitrate-reducing bacteria and their involvement in oxygen-independent iron cycling. Geomicrobiology Journal, 21, 371-378.
38. Torsvik, V., Salte, K., Sorheim, R., & Goksoyr, J. (1990). Comparison of phenotypic diversity and DNA heterogeneity in a population of soil bacteria. Applied and Environmental Microbiology, 56, 776-781.
39. Vieth, A., Kästner, M., Schirmer, M., Weiß, H., Gödeke, S., Meckenstock, R. U., et al. (2005). In-situ biodegradation of benzene and toluene in a contaminated aquifer monitored by stable isotope fractionation. Environmental Toxicology and Chemistry, 24, 51-60.
40. Vogt, C., Alfreider, A., Lorbeer, H., Ahlheim, J., Feist, B., Böhme, O., et al. (2002). Two pilot plant reactors designed for the in situ bioremediation of chlorobenzene-contaminated ground water: Hydro-geological and chemical characteristics and bacterial consortia. Water, Air, & Soil Pollution: Focus, 2, 161-170.
41. Vogt, C., Alfreider, A., Lorbeer, H., Hoffmann, D., Wünsche, L., & Babel, W. (2004). Bioremediation of chlorobenzene-contaminated ground water in an in situ reactor mediated by hydrogen peroxide. Journal of Contaminant Hydrology, 68, 121-141.
42. Vogt, C., Gödeke, S., Treutler, H. C., Weiß, H., Schirmer, M., & Richnow, H. H. (2007). Benzene oxidation under sulfate reducing conditions in columns simulating in situ conditions. Biodegradation, doi: 10.1007/s10532-006-9095-1.
43. Von Wintzingerode, F., Selent, B., Hegemann, W., & Göbel, U. B. (1999). Phylogenetic analysis of an anaerobic, trichlorobenzene-transforming microbial consortium. Applied and Environmental Microbiology, 65, 283-286.
44. Wagner, M., Amann, R., Lemmer, H., & Schleifer, K. H. (1993). Probing activated sludge with oligonucleotides specific for proteobacteria: Inadequacy of culture-dependent methods for describing microbial community structure. Applied and Environmental Microbiology, 59, 1520-1525.
45. Wilson, L. P., & Bouwer, E. J. (1997). Biodegradation of aromatic compounds under mixed oxygen/denitrifying conditions: A review. Journal of Industrial Microbiology & Biotechnology, 18, 116-130.