Real-time profiling of NK cell killing of human astrocytes using xCELLigence technology

Journal of Neuroscience Methods

Volumn 200, Issue 2, 2011, Pages 173-180

  Moodley, Kriebashne ^a   Angel, Catherine E ^b   Glass, Michelle ^a   Graham, E Scott ^a  

^a UNIVERSITY OF AUCKLAND   (New Zealand)

^b UNIVERSITY OF AUCKLAND   (New Zealand)

Author keywords

Brain; Cell death; Cytotoxicity; Glia; Human; Immunology

Indexed keywords

INTERLEUKIN 2; LACTATE DEHYDROGENASE; LYMPHOKINE;

ARTICLE; ASTROCYTE; BIOSENSOR; BIOTECHNOLOGY; CARCINOGENESIS; CELL ADHESION; CELL ASSAY; CELL CULTURE; CELL KILLING; COMPUTER PROGRAM; CONTROLLED STUDY; CYTOTOXICITY; ENZYME RELEASE; HUMAN; HUMAN CELL; IMMUNOCYTOCHEMISTRY; IMPEDANCE; LYMPHOKINE ACTIVATED KILLER CELL; MEASUREMENT; MICROSCOPY; NATURAL KILLER CELL; NERVE CELL DIFFERENTIATION; PHENOTYPE; PRIORITY JOURNAL; SENSITIVITY ANALYSIS; SIGNAL TRANSDUCTION; TARGET CELL DESTRUCTION; TIME LAPSE IMAGING;

EID: 80051593626     PISSN: 01650270     EISSN: 1872678X     Source Type: Journal    
DOI: 10.1016/j.jneumeth.2011.07.005     Document Type: Article

References (30)

1. Bani-Yaghoub M., Felker J.M., Naus C.C. Human NT2/D1 cells differentiate into functional astrocytes. Neuroreport 1999, 10:3843-3846.
2. Brehin A.C., Mouries J., Frenkiel M.P., Dadaglio G., Despres P., Lafon M., et al. Dynamics of immune cell recruitment during West Nile encephalitis and identification of a new CD19+B220-BST-2+leukocyte population. J Immunol 2008, 180:6760-6767.
3. Caligiuri M.A. Human natural killer cells. Blood 2008, 112:461-469.
4. Darlington P.J., Podjaski C., Horn K.E., Costantino S., Blain M., Saikali P., et al. Innate immune-mediated neuronal injury consequent to loss of astrocytes. J Neuropathol Exp Neurol 2008, 67:590-599.
5. Ehlers A., Stempin S., Al-Hamwi R., Lampen A. Embryotoxic effects of the marine biotoxin okadaic acid on murine embryonic stem cells. Toxicon 2010, 55:855-863.
6. Goodfellow C.E., Graham S.E., Dragunow M., Glass M. Characterization of NTera2/D1 cells as a model system for the investigation of cannabinoid function in human neurons and astrocytes. J Neurosci Res 2011.
7. Graham E.S., Angel C.E., Schwarcz L.E., Dunbar P.R., Glass M. Detailed characterisation of CB2 receptor protein expression in peripheral blood immune cells from healthy human volunteers using flow cytometry. Int J Immunopathol Pharmacol 2010, 23:25-34.
8. Graham E.S., Ball N., Scotter E.L., Narayan P., Dragunow M., Glass M. Induction of Krox-24 by endogenous cannabinoid type 1 receptors in Neuro2A cells is mediated by the MEK-ERK MAPK pathway and is suppressed by the phosphatidylinositol 3-kinase pathway. J Biol Chem 2006, 281:29085-29095.
9. Hanusova V., Kralova V., Schroterova L., Trilecova L., Pakostova A., Skalova L. The effectiveness of oracin in enhancing the cytotoxicity of doxorubicin through the inhibition of doxorubicin deactivation in breast cancer MCF7 cells. Xenobiotica 2010, 40:681-690.
10. Hatalski C.G., Hickey W.F., Lipkin W.I. Evolution of the immune response in the central nervous system following infection with Borna disease virus. J Neuroimmunol 1998, 90:137-142.
11. Hedtjarn M., Mallard C., Hagberg H. Inflammatory gene profiling in the developing mouse brain after hypoxia-ischemia. J Cereb Blood Flow Metab 2004, 24:1333-1351.
12. Ke N., Wang X., Xu X., Abassi Y.A. The xCELLigence system for real-time and label-free monitoring of cell viability. Methods Mol Biol 2011, 740:33-43.
13. Keogh R.J. New technology for investigating trophoblast function. Placenta 2010, 31:347-350.
14. Larsson L.C., Czech K.A., Brundin P., Widner H. Intrastriatal ventral mesencephalic xenografts of porcine tissue in rats: immune responses and functional effects. Cell Transplant 2000, 9:261-272.
15. Lim J.H., Gibbons H.M., O'Carroll S.J., Narayan P.J., Faull R.L., Dragunow M. Extracellular signal-regulated kinase involvement in human astrocyte migration. Brain Res 2007, 1164:1-13.
16. Liu C., Luo D., Streit W.J., Harrison J.K. CX3CL1 and CX3CR1 in the GL261 murine model of glioma: CX3CR1 deficiency does not impact tumor growth or infiltration of microglia and lymphocytes. J Neuroimmunol 2008, 198:98-105.
17. Lunemann A., Lunemann J.D., Roberts S., Messmer B., Barreira da Silva R., Raine C.S., et al. cells kill resting but not activated microglia via NKG2D- and NKp46-mediated recognition. J Immunol 2008, 181:6170-6177.
18. Mittelbronn M., Schittenhelm J., Bakos G., de Vos R.A., Wehrmann M., Meyermann R., et al. CD8(+)/perforin/granzyme B(+) effector cells infiltrating cerebellum and inferior olives in gluten ataxia. Neuropathology 2010, 30:92-96.
19. Mittelbronn M., Simon P., Loffler C., Capper D., Bunz B., Harter P., et al. Elevated HLA-E levels in human glioblastomas but not in grade I to III astrocytomas correlate with infiltrating CD8+cells. J Neuroimmunol 2007, 189:50-58.
20. Prat A., Biernacki K., Wosik K., Antel J.P. Glial cell influence on the human blood-brain barrier. Glia 2001, 36:145-155.
21. Rahim S., Uuml Ren A. A real-time electrical impedance based technique to measure invasion of endothelial cell monolayer by cancer cells. J Vis Exp 2011.
22. Saikali P., Antel J.P., Newcombe J., Chen Z., Freedman M., Blain M., et al. NKG2D-mediated cytotoxicity toward oligodendrocytes suggests a mechanism for tissue injury in multiple sclerosis. J Neurosci 2007, 27:1220-1228.
23. Sandhu J.K., Sikorska M., Walker P.R. Characterization of astrocytes derived from human NTera-2/D1 embryonal carcinoma cells. J Neurosci Res 2002, 68:604-614.
24. Slanina H., Konig A., Claus H., Frosch M., Schubert-Unkmeir A Real-time impedance analysis of host cell response to meningococcal infection. J Microbiol Methods 2011, 84:101-108.
25. Smout M.J., Kotze A.C., McCarthy J.S., Loukas A A novel high throughput assay for anthelmintic drug screening and resistance diagnosis by real-time monitoring of parasite motility. PLoS Negl Trop Dis 2010, 4:e885.
26. Ungefroren H., Sebens S., Groth S., Gieseler F., Fandrich F. Differential roles of Src in transforming growth factor-ss regulation of growth arrest, epithelial-to-mesenchymal transition and cell migration in pancreatic ductal adenocarcinoma cells. Int J Oncol 2011.
27. Unsworth C.P., Graham E.S., Delivopoulos E., Dragunow M., Murray A.F. First human hNT neurons patterned on parylene-C/silicon dioxide substrates: combining an accessible cell line and robust patterning technology for the study of the pathological adult human brain. J Neurosci Methods 2010, 194:154-157.
28. Unsworth C.P., Holloway H., Delivopoulos E., Murray A.F., Simpson M.C., Dickinson M.E., et al. Patterning and detailed study of human hNT astrocytes on parylene-C/silicon dioxide substrates to the single cell level. Biomaterials 2011.
29. Urcan E., Haertel U., Styllou M., Hickel R., Scherthan H., Reichl F.X. Real-time xCELLigence impedance analysis of the cytotoxicity of dental composite components on human gingival fibroblasts. Dent Mater 2010, 26:51-58.
30. Vaquero J., Coca S., Oya S., Martinez R., Ramiro J., Salazar F.G. Presence and significance of NK cells in glioblastomas. J Neurosurg 1989, 70:728-731.