First human hNT neurons patterned on parylene-C/silicon dioxide substrates: Combining an accessible cell line and robust patterning technology for the study of the pathological adult human brain

Journal of Neuroscience Methods

Volumn 194, Issue 1, 2010, Pages 154-157

  Unsworth, C P ^a   Graham, E S ^a   Delivopoulos, E ^b   Dragunow, M ^a   Murray, A F ^c  

^a UNIVERSITY OF AUCKLAND   (New Zealand)

^b UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^c UNIVERSITY OF EDINBURGH   (United Kingdom)

Author keywords

Cell patterning; HNT neuron; Human brain cell; NT2; NTera2 cell line; On chip; Parylene; Parylene C; Silicon chip

Indexed keywords

SILICON DIOXIDE;

ARTICLE; BRAIN NERVE CELL; CANCER CELL CULTURE; HISTOPATHOLOGY; HUMAN; IMMERSION; MICROCHIP ANALYSIS; MICROTECHNOLOGY; NEUROIMAGING; PRIORITY JOURNAL; TERATOCARCINOMA;

ANIMALS; BRAIN; BRAIN NEOPLASMS; CELL DIFFERENTIATION; CELL LINE, TUMOR; CYTOLOGICAL TECHNIQUES; HUMANS; LAB-ON-A-CHIP DEVICES; MICROCOMPUTERS; MICROTECHNOLOGY; NEURONS; POLYMERS; RATS; RATS, SPRAGUE-DAWLEY; SILICON DIOXIDE; TERATOMA; XYLENES;

EID: 78649524002     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jneumeth.2010.09.022     Document Type: Article

References (11)

1. Andrews P.W. Retinoic acid induces neuronal differentiation of a cloned human embryonal carcinoma cell line in vitro. Dev Biol 1984, 103:285-293.
2. Delivopoulos E., Murray A.F., MacLeod N.K., Curtis J.C. Guided growth of neurons and glia using microfabricated patterns of parylene-C on a SiO2 background. Biomaterials 2009, 30:2048-2058.
3. Görtz P., Fleischer W., Rosenbaum C., Otto F., Siebler M. Neuronal properties of human teratocarcinoma cell line-derived neurons. Brain Res 2004, 1018:18-25.
4. Howard B.M., Mo Z., Filipovic R., Moore A.R., Antic S.D., Zecevic N. Radial glia cells in the developing human brain. Neuroscientist 2008, 14(5):459-473.
5. Lim J.H., Gibbons H.M., O'Carroll S.J., Narayan P.J., Faull R.L.M., Dragunow M. Extracellular signal-regulated kinase involvement in human astrocyte migration. Brain Res 2007, 1164(1):1-13.
6. Murray AF. 'Cell patterning'. UK patent application number: 0820572.6. Filed 11th November 2008.
7. Palmer T.D., Schwartz P.H., Taupin P., Kaspar B. Progenitor cells from human brain after death. Nature 2001, 411:42-43.
8. Pleasure S.J., Page C.D., Lee V.M. Pure, postmitotic polarized human neurons derived from Ntera2 cells provide a system for expressing exogenous proteins in terminally differentiated neurons. J Neurosci 1992, 12:1802-1815.
9. Regan E.M. Differential patterning of neuronal, glial and neural progenitor cells on phosphorus-doped and UV irradiated diamond-like carbon. Biomaterials 2010, 31(2):207-215.
10. Svendsen C.N., Ter Borg M.G., Armstrong R.J.E., Rosser A.E., Chandran S., Ostenfeld T., et al. A new method for the rapid and long term growth of human neural precursor cells. J Neurosci Met 1998, 85(2):141-152.
11. Wheeler B.C., Brewer G.J. Designing neural networks in culture. Proc IEEE 2010, 98(3):398-406.