Detailed characterisation of CB2 receptor protein expression in peripheral blood immune cells from healthy human volunteers using flow cytometry

International Journal of Immunopathology and Pharmacology

Volumn 23, Issue 1, 2010, Pages 25-34

  Graham, E Scott ^a   Angel, C E ^b   Schwarcz, L E ^a   Dunbar, P R ^a,b   Glass, M ^a  

^a UNIVERSITY OF AUCKLAND   (New Zealand)

^b UNIVERSITY OF AUCKLAND   (New Zealand)

Author keywords

Cannabinoids; CB1; CB2; Human; Immune cells; Leukocytes

Indexed keywords

CANNABINOID 2 RECEPTOR; ENDOCANNABINOID; POLYCLONAL ANTIBODY;

ARTICLE; B LYMPHOCYTE; CD4+ T LYMPHOCYTE; CD8+ T LYMPHOCYTE; CELL SPECIFICITY; CELL SURFACE; CONTROLLED STUDY; FLOW CYTOMETRY; HUMAN; HUMAN CELL; HUMAN EXPERIMENT; IMMUNOCOMPETENT CELL; INFLAMMATION; LEUKOCYTE; MONOCYTE; NATURAL KILLER CELL; NEUTROPHIL; NORMAL HUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; STAINING;

EID: 77951703978     PISSN: 03946320     EISSN: None     Source Type: Journal    
DOI: 10.1177/039463201002300103     Document Type: Article

References (36)

1. Munro S, Thomas KL, Abu-Shaar M. Molecular characterization of a peripheral receptor for cannabinoids. Nature 1993;365:61-65 (Pubitemid 23305569)
2. Matsuda LA. Molecular aspects of cannabinoid receptors. Crit Rev Neurobiol 1997;11:143-166
3. Cabral GA, Raborn ES, Griffin L, Dennis J, Marciano-Cabral F. CB2 receptors in the brain: role in central immune function. Br J Pharmacol 2008;153:240-251
4. Mackie K. Cannabinoid receptors as therapeutic targets. Annu Rev Pharmacol Toxicol 2006;46:101-122
5. Fernandez-Ruiz J, Pazos MR, Garcia-Arencibia M, Sagredo O, Ramos JA. Role of CB2 receptors in neuroprotective effects of cannabinoids. Mol Cell Endocrinol 2008;286:S91-6.
6. Guindon J, Hohmann AG. Cannabinoid CB2 receptors: a therapeutic target for the treatment of inflammatory and neuropathic pain. Br J Pharmacol 2008;153:319-334
7. Galiegue S, Mary S, Marchand J, et al. Expression of central and peripheral cannabinoid receptors in human immune tissues and leukocyte subpopulations. Eur J Biochem 1995;232:54-61.
8. Bai M, Sexton M, Stella N, Bornhop DJ. MBC94, a conjugable ligand for cannabinoid CB 2 receptor imaging. Bioconjug Chem 2008;19:988-992 (Pubitemid 351748305)
9. Gonsiorek W, Hesk D, Chen SC, et al. Characterization of peripheral human cannabinoid receptor (hCB2) expression and pharmacology using a novel radioligand, [35S]Sch225336. J Biol Chem 2006;281:28143-28151 (Pubitemid 44414528)
10. Arevalo-Martin A, Vela JM, Molina-Holgado E, Borrell J, Guaza C. Therapeutic action of cannabinoids in a murine model of multiple sclerosis. J Neurosci 2003;23:2511-2516 (Pubitemid 36418583)
11. Zhang M, Martin BR, Adler MW, Razdan RK, Ganea D, Tuma RF. Modulation of the balance between cannabinoid CB(1) and CB(2) receptor activation during cerebral ischemic/reperfusion injury. Neuroscience 2008;152:753-760
12. Zhang M, Martin BR, Adler MW, Razdan RK, Jallo JI, Tuma RF. Cannabinoid CB(2) receptor activation decreases cerebral infarction in a mouse focal ischemia/reperfusion model. J Cereb Blood Flow Metab 2007;27:1387-1396
13. Baldwin GC, Tashkin DP, Buckley DM, Park AN, Dubinett SM, Roth MD. Marijuana and cocaine impair alveolar macrophage function and cytokine production. Am J Respir Crit Care Med 1997;156:1606-1613
14. Gonsiorek W, Lunn CA, Fan X, Deno G, Kozlowski J, Hipkin RW. Sch35966 is a potent, selective agonist at the peripheral cannabinoid receptor (CB2) in rodents and primates. Br J Pharmacol 2007;151:1262-1271 (Pubitemid 47255903)
15. Nunez E, Benito C, Pazos MR, Barbachano A, Fajardo O, Gonzalez S, Tolon RM, Romero J. Cannabinoid CB2 receptors are expressed by perivascular microglial cells in the human brain: an immunohistochemical study. Synapse 2004;53:208-213 (Pubitemid 39045539)
16. Jbilo O, Derocq JM, Segui M, Le Fur G, Casellas P. Stimulation of peripheral cannabinoid receptor CB2 induces MCP-I and IL-8 gene expression in human promyelocytic cell line HL60. FEBS Lett 1999;448:273-277 (Pubitemid 29168502)
17. Lunn CA, Fine JS, Rojas-Triana A, et al. A novel cannabinoid peripheral cannabinoid receptor-selective inverse agonist blocks leukocyte recruitment in vivo. J Pharmacol Exp Ther 2006;316:780-788 (Pubitemid 43131006)
18. Lunn CA, Reich EP, Fine JS, Lavey B, Kozlowski JA, Hipkin RW, Lundell DJ, Bober L. Biology and therapeutic potential of cannabinoid CB2 receptor inverse agonists. Br J Pharmacol 2008;153:226-239
19. Bouaboula M, Rinaldi M, Carayon P, Carillon C, Delpech B, Shire D, Le Fur G, Casellas P. Cannabinoid-receptor expression in human leukocytes. Eur J Biochem 1993;214:173-180
20. Nong L, Newton C, Cheng Q, Friedman H, Roth MD, Klein TW Altered cannabinoid receptor mRNA expression in peripheral blood mononuclear cells from marijuana smokers. J Neuroimmunol 2002;127:169-176 (Pubitemid 34603588)
21. Rayman N, Lam KH, Van Leeuwen J, Mulder AH, Budel LM, Lowenberg B, Sonneveld P, Delwel R. The expression of the peripheral cannabinoid receptor on cells of the immune system and non-Hodgkin's lymphomas. Leuk Lymphoma 2007;48:1389-1399 (Pubitemid 47079367)
22. Stander S, Schmelz M, Metze D, Luger T, Rukwied R. Distribution of cannabinoid receptor 1 (CB1) and 2 (CB2) on sensory nerve fibers and adnexal structures in human skin. J Dermatol Sci 2005;38:177-188 (Pubitemid 40779571)
23. Nunez E, Benito C, Tolon RM, Hillard CJ, Griffin WS, Romero J. Glial expression of cannabinoid CB(2) receptors and fatty acid amide hydrolase are beta amyloid-linked events in Down's syndrome. Neuroscience 2008;151:104-110
24. Benito C, Romero JP, Tolon RM, Clemente D, Docagne F, Hillard CJ, Guaza C, Romero J. Cannabinoid CB1 and CB2 receptors and fatty acid amide hydrolase are specific markers of plaque cell subtypes in human multiple sclerosis. J Neurosci 2007;27:2396-2402
25. Ashton JC, Rahman RM, Nair SM, Sutherland BA, Glass M, Appleton I. Cerebral hypoxia-ischemia and middle cerebral artery occlusion induce expression of the cannabinoid CB2 receptor in the brain. Neurosci Lett 2007;412:114-117 (Pubitemid 46074356)
26. Ashton JC, Glass M. The Cannabinoid CB2 Receptor as a target for inflammation-dependent neurodegeneration. Curr Neuropharmacol 2007;5:73-80. (Pubitemid 47019422)
27. Abdi R, Fiorina P, Adra CN, Atkinson M, Sayegh MH. Immunomodulation by mesenchymal stem cells: a potential therapeutic strategy for type 1 diabetes. Diabetes 2008;57:1759-1767
28. Angel CE, George E, Brooks AE, Ostrovsky LL, Brown TL, Dunbar PR. Cutting edge: CD1a+ antigen-presenting cells in human dermis respond rapidly to CCR7 ligands. J Immunol 2006;176:5730-5734
29. Angel CE, Lala A, Chen CJ, Edgar SG, Ostrovsky LL, Dunbar PR. CD 14+ antigen-presenting cells in human dermis are less mature than their CD1a+ counterparts. Int Immunol 2007;19:1271-1279 (Pubitemid 47604728)
30. Mardiney M, 3rd, Brown MR, Fleisher TA. Measurement of T-cell CD69 expression: a rapid and efficient means to assess mitogen- or antigen-induced proliferative capacity in normals. Cytometry 1996;26:305-310
31. Bogunia-Kubik K, Natarajan P, Madrigal JA, Cohen SB. The effect of cord blood sera on CD69 expression. Immunol Lett 2002;84:77-80. (Pubitemid 34879923)
32. Coopman K, Smith LD, Wright KL, Ward SG. Temporal variation in CB2R levels following T lymphocyte activation: evidence that cannabinoids modulate CXCL12-induced Chemotaxis. Int Immunopharmacol 2007;7:360-371 (Pubitemid 46186175)
33. Faucher JL, Lacronique-Gazaille C, Frebet E, Trimoreau F, Donnard M, Bordessoule D, Lacombe F, Feuillard J. "6 markers/5 colors" extended white blood cell differential by flow cytometry. Cytometry A 2007;71:934-944
34. Michel M, Wieland T, Tsujimoto G. How reliable are G-protein-coupled receptor antibodies. Naunyn-Schmied Arch Pharmacol 2009;379:385-388
35. Grimsey NL, Goodfellow CE, Scotter EL, Dowie MJ, Glass M, Graham ES. Specific detection of CB1 receptors; cannabinoid CB1 receptor antibodies are not all created equal! J Neurosci Methods 2008;171:78-86.
36. Montecucco F, Burger F, Mach F, Steffens S. CB2 cannabinoid receptor agonist JWH-015 modulates human monocyte migration through defined intracellular signaling pathways. Am J Physiol Heart Circ Physiol 2008;294:1145-1155 (Pubitemid 351468709)