A novel method for producing mono-biotinylated, biologically active neurotrophic factors: An essential reagent for single molecule study of axonal transport

Journal of Neuroscience Methods

Volumn 200, Issue 2, 2011, Pages 121-128

  Sung, Kijung ^a   Maloney, Michael T ^a   Yang, Jingkun ^b   Wu, Chengbiao ^c  

^a STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

^c UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Axonal transport; Biotinylation; Dorsal root ganglion; Microfluidic nerve cell chamber; Nerve growth factor; PC12 cells; Quantum dots; Receptors; Signaling pathways; Single molecule imaging

Indexed keywords

BIOTIN; COMPLEMENTARY DNA; LIGASE; MOLECULE TRANSPORT AGENT; NERVE GROWTH FACTOR; NEUROTROPHIC FACTOR; NEUROTROPIN; QUANTUM DOT; REAGENT; RESIN; STREPTAVIDIN;

AMINO TERMINAL SEQUENCE; ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BIOTINYLATION; CELL CULTURE; CELL STRAIN HEK293; DNA VECTOR; ESCHERICHIA COLI; HUMAN; HUMAN CELL; NERVE FIBER TRANSPORT; NONHUMAN; PRIORITY JOURNAL; PROTEIN SECRETION; PURIFICATION; RAT; SIGNAL TRANSDUCTION; SPINAL GANGLION; SUBMANDIBULAR GLAND;

ANIMALS; AXONS; BIOLOGICAL TRANSPORT, ACTIVE; BIOTINYLATION; CARBON-NITROGEN LIGASES; CELLS, CULTURED; CLONING, MOLECULAR; EMBRYO, MAMMALIAN; ESCHERICHIA COLI PROTEINS; GANGLIA, SPINAL; GREEN FLUORESCENT PROTEINS; HUMANS; MICE; MICROSCOPY, CONFOCAL; MOLECULAR PROBE TECHNIQUES; NERVE GROWTH FACTOR; NEURONS; RATS; REPRESSOR PROTEINS; TRANSFECTION;

EID: 80051577633     PISSN: 01650270     EISSN: 1872678X     Source Type: Journal    
DOI: 10.1016/j.jneumeth.2011.06.020     Document Type: Article

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