DNA methylation levels in porcine fetal fibroblasts induced by an inhibitor of methylation, 5-azacytidine

Cell and Tissue Research

Volumn 325, Issue 3, 2006, Pages 445-454

  Mohana Kumar, B ^a   Jin, H F ^a   Kim, J G ^a   Song, H J ^a   Hong, Y ^b   Balasubramanian, S ^a,c   Choe, S Y ^a   Rho, G J ^a  

^a Gyeongsang National University   (South Korea)

^b Inje University   (South Korea)

^c MADRAS VETERINARY COLLEGE   (India)

Author keywords

5 Azacytidine; Cell cycle; DNA methylation; Fetal fibroblasts; Porcine

Indexed keywords

AZACITIDINE; DNA; DNA METHYLTRANSFERASE 1; DNA METHYLTRANSFERASE INHIBITOR; METHYL GROUP; PROTEIN BAX; PROTEIN BCL 2;

ANIMAL CELL; APOPTOSIS; ARTICLE; CELL CULTURE; CELL CYCLE G1 PHASE; CELL FRACTIONATION; CELL STRUCTURE; CONFOCAL MICROSCOPY; CONTROLLED STUDY; DNA METHYLATION; DNA REPLICATION; EXPOSURE; FEMALE; FETUS; FIBROBLAST; GENE EXPRESSION; GENE INDUCTION; GENOME IMPRINTING; NICK END LABELING; NONHUMAN; NUCLEOTIDE SEQUENCE; PLOIDY; PRIORITY JOURNAL; PROTEIN EXPRESSION; SWINE;

ANIMALS; APOPTOSIS; AZACITIDINE; CELL CYCLE; CELL DIVISION; CELLS, CULTURED; DNA METHYLATION; DOSE-RESPONSE RELATIONSHIP, DRUG; FEMALE; FIBROBLASTS; FLOW CYTOMETRY; MICROSCOPY, CONFOCAL; PLOIDIES; PREGNANCY; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; SWINE; TIME FACTORS;

SUIDAE;

EID: 33746456410     PISSN: 0302766X     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00441-006-0201-9     Document Type: Article

References (42)

1. Beaujean N, Hartshorne G, Cavilla J, Taylor J, Gardner J, Wilmut I, Meehan R, Young LE (2004) Non-conservation of mammalian preimplantation methylation dynamics. Curr Biol 14:R266-R267
2. Bird A (2002) DNA methylation patterns and epigenetic memory. Genes Dev 16:6-21
3. Boquest AC, Day BN, Prather RS (1999) Flow cytometric analysis of cultured porcine fetal fibroblast cells. Biol Reprod 60:1013-1019
4. Boquest AC, Grupen CG, Harrison SJ, Mcllfatrick SM, Ashman RJ, d'Apice AJF, Nottle MB (2002) Production of cloned pigs from cultured fetal fibroblast cells. Biol Reprod 66:1283-1287
5. Bour'chis D, LeBourhis D, Patin D, Niveleau A, Cornizolli P, Renard JP, Viegas-Pequignot E (2001) Delayed and incomplete reprogramming of chromosome methylation patterns in bovine embryos. Curr Biol 11:1542-1546
6. Brison DR, Schultz RM (1997) Apoptosis during mouse blastocyst ormation: evidence for a role for survival factors including transforming growth factor a. Biol Reprod 56:1088-1096
7. Christman JK (2002) 5-Azacytidine and 5-aza-2-deoxycytidine as inhibitors of DNA methylation: mechanistic studies and their implications for cancer therapy. Oncogene 21:5483-5495
8. Cooper S (1998) On the interpretation of the shortening of the G1-phase by overexpression of cyclins in mammalian cells. Exp Cell Res 238:110-115
9. Dean W, Santos F, Stojkovic M, Zakhartchenko V, Walter J, Wolf E, Reik W (2001) Conservation of methylation reprogramming in mammalian development: aberrant reprogramming in cloned embryos. Proc Natl Acad Sci USA 98:13734-13738
10. Dean W, Santos F, Reik W (2003) Epigenetic reprogramming in early mammalian development and following somatic nuclear transfer. Semin Cell Dev Biol 14:93-100
11. Enright BP, Kubota C, Yang X, Tian XC (2003) Epigenetic characteristics and development of embryos cloned from donor cells treated by trichostatin A or 5-aza-2′-deoxycytidine. Biol Reprod 69:896-901
12. Enright BP, Sung LY, Chang CC, Yang X, Tian XC (2005) Methylation and acetylation characteristics of cloned bovine embryos from donor cells treated with 5-aza-2′-deoxycytidine. Biol Reprod 72:944-948
13. Fairburn HR, Young LE, Hendrich BD (2002) Epigenetic reprogramming: how now, cloned cow? Curr Biol 12:R68-R70
14. Haaf T, Schmidt M (1989) 5-Azadeoxycytidine induced under condensation in the giant X chromosomes of Microtus agrestis. Chromosoma 98:93-98
15. Hu JF, Nguyen PH, Pharn NV, Vu TH, Hoffman AR (1997) Modulation of Igf2 genomic imprinting in mice induced by 5-azacytidine, an inhibitor of DNA methylation. Mol Endocrinol 11:1891-1898
16. Humpherys D, Eggan K, Akutsu H, Hochedlinger K, Rideout WM 3rd, Biniszkiewicz D, Yanagimachi R, Jaenisch R (2001) Epigenetic instability in ES cells and cloned mice. Science 293:95-97
17. Jablonka E, Goitein R, Marcus M, Cedar H (1985) DNA hypomethylation causes an increase in DNase-I sensitivity and an advance in the time of replication of the entire inactive chromosome. Chromosoma 93:152-156
18. Jackson-Grusby L, Beard C, Possemato R, Tudor M, Fambrough D, Csankovszki G, Dausman J, Lee P, Wilson C, Lander E, Jaenisch R (2001) Loss of genomic methylation causes p53-dependent apoptosis and epigenetic deregulation. Nat Genet 27:31-39
19. Jones KL, Hill J, Shin TY, Lui L, Westhusin M (2001) DNA hypomethylation of karyoplasts for bovine nuclear transfer. Mol Reprod Dev 60:208-213
20. Kang YK, Park JS, Choi YH, Chung AS, Lee KK, Han YM (2001a) Aberrant methylation of donor genome in cloned bovine embryos. Nat Genet 28:173-177
21. Kang YK, Koo DB, Park JS, Choi YH, Kim SU, Chang WK, Lee KK, Han YM (2001b) Typical demethylation events in cloned pig embryos. Clues on species-specific differences in epigenetic reprogramming of a cloned donor genome. J Biol Chem 276:39980-39984
22. Kang YK, Park JS, Koo DB, Choi YH, Kim SU, Lee KK, Han YM (2002) Limited demethylation leaves mosaic-type methylation states in bovine pre-implantation embryos. EMBO J 21:1092-1100
23. Kharroubi AE, Piras G, Stewart CL (2001) DNA demethylation reactivates a subset of imprinted genes in uniparental mouse embryonic fibroblasts. J Biol Chem 276:8674-8680
24. King WA, Linares T, Gustavsson I, Bane AA (1979) Method for preparation of chromosomes from bovine zygotes and blastocysts. Vet Sci Commun 3:51-56
25. Li E (2002) Chromatin modification and epigenetic reprogramming in mammalian development. Nat Rev Genet 3:662-673
26. Mayer W, Niveleau A, Walter J, Fundele R, Haaf T (2000) Demethylation of the zygotic paternal genome. Nature 403:501-502
27. Memili E, First NL (2000) Zygotic and embryonic gene expression in cow: a review of timing and mechanisms of early gene expression as compared with other species. Zygote 8:87-96
28. Morgan HD, Santos F, Green K, Dean W, Reik W (2005) Epigenetic reprogramming in mammals. Hum Mol Gen 14:R47-R58
29. Ohgane J, Wakayama T, Kogo Y, Seneda S, Hattori N, Tanaka S, Yanagimachi R, Shiota K (2001) DNA methylation variation in cloned mice. Genesis 30:45-50
30. Oswald J, Engemann S, Lane N, Mayer W, Olek A, Fundele R, Dean W, Reik W, Walter J (2000) Active demethylation of the parental genome in the mouse zygote. Curr Biol 10:475-478
31. Pedone PV, Pikaart MJ, Cerrato F, Vernucci M, Ungaro P, Bruni CB, Riccio A (1999) Role of histone acetylation and DNA methylation in the maintenance of imprinted expression of the H19 and Igf2 genes. FEBS Lett 458:45-50
32. Petti MC, Mandelli F, Zagonel V, De Gregoris C, Merola MC, Latagne R, Gattei V, Fazi P, Monfardini S, Pinto A (1993) Pilot study of 5-aza-2-deoxycytidine (decitabine) in the treatment of poor prognosis acute myelogenous leukemia patients: preliminary results. Leukemia 1(Suppl):36-41
33. Polejaeva IA, Chen SH, Vaught TD, Page RL, Mullins J, Ball S, Dai YF, Boone J, Walker S, Ayares DL, Colman A, Campbell KH (2000) Cloned pigs produced by nuclear transfer from adult somatic cells. Nature 407:86-90
34. Reik W, Dean W (2001) DNA methylation and mammalian epigenetics. Electrophoresis 22:2838-2843
35. Reik W, Walter J (2001) Genomic imprinting: parental influence on the genome. Nat Rev Genet 2:21-32
36. Santini V, Kantarjian HM, Issa JP (2001) Changes in DNA methylation in neoplasia: pathophysiology and therapeutic implications. Ann Intern Med 134:573-586
37. Santos F, Dean W (2004) Epigenetic reprogramming during early development in mammals. Reproduction 127:643-651
38. Santos F, Hendrich B, Reik W, Dean W (2002) Dynamic reprogramming of DNA methylation in the early mouse embryo. Dev Biol 241:172-182
39. Santos F, Zakhartchenko V, Stojkovic M, Peters A, Jenuwein T, Wolf E, Reik W, Dean W (2003) Epigenetic marking correlates with developmental potential in cloned bovine preimplantation embryos. Curr Biol 13:1116-1121
40. Schneider-Stock R, Diab-Assef M, Rohrbeck A, Foltzer-Jourdainne C, Boltze C, Hartig R, Schonfeld P, Roessner A, Gali-Muhtasib H (2005) 5-Azacytidine is a potent inhibitor of DNA methyltransferase 3a and induces apoptosis in HCT-116 colon cancer cells via Gadd45- and p53-dependent mechanisms. J Pharma Exp Ther 312:525-536
41. Shi W, Zakhartchenko V, Wolf E (2003a) Epigenetic reprogramming in mammalian nuclear transfer. Differentiation 71:91-113
42. Shi W, Hoeflich A, Flaswinkel H, Stojkovic M, Wolf E, Zakhartchenko V (2003b) Induction of a senescent-like phenotype does not confer the ability of bovine immortal cells to support the development of nuclear transfer embryos. Biol Reprod 69:374-380