Structural stability of E. coli transketolase to temperature and pH denaturation

Journal of Biotechnology

Volumn 155, Issue 2, 2011, Pages 209-216

  Jahromi, Raha R F ^a   Morris, Phattaraporn ^a   Martinez Torres, Ruben J ^a   Dalby, Paul A ^a  

^a UNIVERSITY COLLEGE LONDON   (United Kingdom)

Author keywords

Biocatalysis; Denaturation; Stability; Transketolase; Tryptophan fluorescence

Indexed keywords

BIOCATALYSIS; COFACTOR BINDING; COFACTORS; COMPLEX MECHANISMS; E. COLI; EFFECTS OF TEMPERATURE; ENZYME STRUCTURES; HIGH PH; HIGHER TEMPERATURES; HOMODIMERS; IN-PROCESS; INCREASED TEMPERATURE; IRREVERSIBLE AGGREGATION; IRREVERSIBLE DENATURATION; PARTIAL DENATURATION; PRE-INCUBATION; PRODUCT STABILITY; PROTEIN DENATURATION; STRUCTURAL CHANGE; STRUCTURAL STABILITIES; TRANSKETOLASE; TRYPTOPHAN FLUORESCENCE; UNDERLYING CAUSE;

AMINO ACIDS; DENATURATION; DISSOCIATION; ENZYMES; ESCHERICHIA COLI; METABOLISM; STABILITY; TEMPERATURE; UREA;

PH EFFECTS;

TRANSKETOLASE;

ARTICLE; BIOCATALYSIS; ENZYME ACTIVITY; ENZYME DENATURATION; ENZYME STABILITY; ESCHERICHIA COLI; HIGH TEMPERATURE; PH; PRIORITY JOURNAL; TEMPERATURE; THERMOSTABILITY;

CIRCULAR DICHROISM; ENZYME STABILITY; ESCHERICHIA COLI; FLUORESCENCE; HYDROGEN-ION CONCENTRATION; MODELS, MOLECULAR; PARTICLE SIZE; PROTEIN DENATURATION; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; TEMPERATURE; TRANSKETOLASE;

ESCHERICHIA COLI;

EID: 80051470803     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2011.06.023     Document Type: Article

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