Effects of transketolase cofactors on its conformation and stability

Life Sciences

Volumn 78, Issue 1, 2005, Pages 8-13

  Esakova, Olga A ^a   Meshalkina, Ludmilla E ^a   Kochetov, German A ^a  

^a MOSCOW STATE UNIVERSITY   (Russian Federation)

Author keywords

Circular dichroism spectroscopy; Conformation; Differential scanning calorimetry; Fluorescence spectroscopy; Folding; Thiamine diphosphate; Transketolase

Indexed keywords

CALCIUM ION; COCARBOXYLASE; DIVALENT CATION; HOLOENZYME; MAGNESIUM ION; TRANSKETOLASE; TRYPTOPHAN;

ARTICLE; CIRCULAR DICHROISM; DATA ANALYSIS; DIFFERENTIAL SCANNING CALORIMETRY; ENZYME ACTIVE SITE; ENZYME ANALYSIS; ENZYME CONFORMATION; ENZYME KINETICS; ENZYME RECONSTITUTION; ENZYME STABILITY; FLUORESCENCE SPECTROSCOPY; PROTEIN SECONDARY STRUCTURE;

CALORIMETRY, DIFFERENTIAL SCANNING; CATIONS; CIRCULAR DICHROISM; COENZYMES; KINETICS; MODELS, MOLECULAR; PROTEIN CONFORMATION; PROTEIN DENATURATION; SACCHAROMYCES CEREVISIAE; SPECTROMETRY, FLUORESCENCE; TEMPERATURE; TRANSKETOLASE; TRYPTOPHAN;

SACCHAROMYCES CEREVISIAE;

EID: 23444455100     PISSN: 00243205     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.lfs.2004.12.055     Document Type: Article

References (24)

1. A.G. Datta, and E. Racker Mechanism of action of transketolase: I. Properties of the crystalline yeast enzyme Journal of Biological Chemistry 236 1961 617 623
2. R.M. Egan, and H.Z. Sable Transketolase kinetics. The slow reconstitution of the holoenzyme is due to rate-limiting dimerization of the subunits Journal of Biological Chemistry 256 1981 4877 4883
3. S. Gerhardt, S. Echt, M. Busch, J. Freigang, G. Auerbach, G. Bader, W.F. Martin, A. Bacher, R. Huber, and M. Fischer Structure and properties of an engineered transketolase from maize Plant Physiology 132 2003 1941 1949
4. N.J. Greenfield Methods to estimate the conformation of proteins and polypeptides from circular dichroism data Analytical Biochemistry 235 1996 1 10
5. P.C. Heinrich, H. Steffen, P. Janser, and O. Wiss Studies on the reconstitution of apotransketolase with thiamine pyrophosphate and analogs of the coenzyme European Journal of Biochemistry 30 1972 533 541
6. C.P. Heinrich, K. Noack, and O. Wiss Chemical modification of tryptophan at the binding site of thiamine-pyrophosphate in transketolase from Baker's yeast Biochemical and Biophysical Research Communications 49 1972 1427 1432
7. G.A. Kochetov Transketolase from yeast, rat liver, and pig liver Methods in Enzymology 90 1982 209 223
8. G.A. Kochetov, and A.E. Izotova Reconstruction of holotransketolase from the apoenzyme and coenzyme Biokhimiia 38 1973 552 560 (in Russian)
9. G.A. Kochetov, and P.P. Philippov Calcium: cofactor of transketolase from baker's yeast Biochemical and Biophysical Research Communications 38 1970 930 933
10. G.A. Kochetov, N.K. Tikhomirova, and P.P. Philippov The binding of thiamine pyrophosphate with transketolase in equilibrium conditions Biochemical and Biophysical Research Communications 63 1975 924 930
11. M.V. Kovina, V.A. Selivanov, N.V. Kochevova, and G.A. Kochetov Kinetic mechanism of active site non-equivalence in transketolase FEBS Letters 418 1997 11 14
12. Y. Lindqvist, G. Schneider, U. Ermler, and M. Sundström Three-dimensional structure of transketolase, a thiamine diphosphate dependent enzyme, at 2.5 A resolution EMBO Journal 11 1992 2373 2379
13. L.E. Meshalkina, and G.A. Kochetov The role of bivalent cations in the interaction of thiamin diphosphate with apotransketolase from baker yeast Doklady Akademii Nauk USSR 248 1979 1482 1486
14. M. Nikkola, Y. Lindqvist, and G. Schneider Refined structure of transketolase from Saccharomyces cerevisiae at 2.0 A resolution Journal of Molecular Biology 238 1994 387 404
15. V.A. Selivanov, M.V. Kovina, N.V. Kochevova, L.E. Meshalkina, and G.A. Kochetov Studies of thiamin diphosphate binding to the yeast apotransketolase Journal of Molecular Catalysis. B, Enzymatic 26 2003 33 40
16. V.A. Selivanov, M.V. Kovina, N.V. Kochevova, L.E. Meshalkina, and G.A. Kochetov Kinetic study of the H103A mutant yeast transketolase FEBS Letters 567 2004 270 274
17. M. Sundström, Y. Lindqvist, and G. Schneider Three-dimensional structure of apotransketolase FEBS Letters 313 1992 229 231
18. C. Tanford Protein denaturation Advances in Protein Chemistry 23 1968 121 282
19. C. Tanford Protein denaturation. C. Theoretical models for the mechanism of denaturation Advances in Protein Chemistry 24 1970 91 95
20. C. Tanford, and P.K. De The unfolding of beta-lactoglobulin at pH 3 by urea, formamide, and other organic substances Journal of Biological Chemistry 236 1961 1711 1715
21. J. Ullrich, and A. Mannschreck Studies on the properties of 2-alpha-hydroxyethyl-thiamine pyrophosphate ("active acetaldehyde") European Journal of Biochemistry 1 1967 110 116
22. R.A. Usmanov, and G.A. Kochetov Study of different conformational states of transketolase by the method of perturbation UV-spectrophotometry Biokhimiia 43 1978 1796 1804 (in Russian)
23. N.J. Veitch, D.A. Maugeri, J.J. Cazzulo, Y. Lindqvist, and M.P. Barrett Transketolase from Leishmania mexicana has a dual subcellular localization Biochemical Journal 382 2004 759 767
24. C. Wikner, L. Meshalkina, U. Nilsson, M. Nikkola, Y. Lindqvist, M. Sundström, and G. Schneider Analysis of an invariant cofactor-protein interaction in thiamin diphosphate-dependent enzymes by site-directed mutagenesis. Glutamic acid 418 in transketolase is essential for catalysis Journal of Biological Chemistry 269 1994 32144 32150