53BP1 and MDC1 foci formation in HT-1080 cells for low- and high-LET microbeam irradiations

Radiation and Environmental Biophysics

Volumn 50, Issue 3, 2011, Pages 345-352

  Mosconi, Marita ^a   Giesen, Ulrich ^a   Langner, Frank ^a   Mielke, Christian ^b   Dalla Rosa, Ilaria ^b   Dirks, Wilhelm G ^c  

^a PHYSIKALISCH TECHNISCHE BUNDESANSTALT   (Germany)

^b HEINRICH HEINE UNIVERSITY   (Germany)

^c LEIBNIZ INSTITUTE DSMZ GERMAN COLLECTION OF MICROORGANISMS AND CELL CULTURES   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

BIOLOGICAL EFFECTS; COLOCALIZATION; DOSE DEPOSITION; EQUAL SIZES; FIBROSARCOMA CELLS; FLUORESCENT PROTEIN; FUSION PROTEINS; GREEN FLUORESCENT PROTEIN; HIERARCHICAL ASSEMBLIES; HT-1080; HT-1080 CELLS; IN-LINE; LINEAR ENERGY TRANSFER; LIVING CELL; LOW DOSE; MICRO BEAMS; MICROBEAM IRRADIATION; PARTICLE IRRADIATION; PRELIMINARY DATA; RADIATION BIOLOGY; RADIATION-INDUCED; RECOMBINANT CELLS; RELATED RISK; WILD TYPES;

CELL CULTURE; CELLS; ENERGY TRANSFER; FLUORESCENCE; IONIZING RADIATION; KINETICS; PROTEINS; PROTONS; RADIATION EFFECTS;

IRRADIATION;

GREEN FLUORESCENT PROTEIN; MDC1 PROTEIN, HUMAN; NUCLEAR PROTEIN; PHOTOPROTEIN; PROTON; RED FLUORESCENT PROTEIN; SIGNAL PEPTIDE; TP53BP1 PROTEIN, HUMAN; TRANSACTIVATOR PROTEIN;

ALPHA RADIATION; ARTICLE; CELL CLONE; CELL SURVIVAL; DNA DAMAGE; GENETICS; HUMAN; KINETICS; LINEAR ENERGY TRANSFER; METABOLISM; MOLECULAR IMAGING; RADIATION RESPONSE; TUMOR CELL LINE;

ALPHA PARTICLES; CELL LINE, TUMOR; CELL SURVIVAL; CLONE CELLS; DNA DAMAGE; DOSE-RESPONSE RELATIONSHIP, RADIATION; GREEN FLUORESCENT PROTEINS; HUMANS; INTRACELLULAR SIGNALING PEPTIDES AND PROTEINS; KINETICS; LINEAR ENERGY TRANSFER; LUMINESCENT PROTEINS; MOLECULAR IMAGING; NUCLEAR PROTEINS; PROTONS; TRANS-ACTIVATORS;

EID: 79961210398     PISSN: 0301634X     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00411-011-0366-9     Document Type: Article

References (26)

1. A Asaithamby DJ Chen 2009 Cellular responses to DNA double-strand breaks after low-dose γ-irradiation Nucleic Acids Res 37 3912 3923 10.1093/nar/gkp237
2. A Asaithamby N Uematsu A Chatterjee MD Story S Burma DJ Chen 2008 Repair of HZE-particle induced DNA double strand breaks in normal human fibroblasts Radiat Res 169 437 446 10.1667/RR1165.1 (Pubitemid 351620042)
3. M Audebert B Salles P Calsou 2004 Involvement of poly(ADP-ribose) Polymerase-1 and XRCC1/DNA Ligase III in an alternative route for DNA Double strand breaks rejoining J Biol Chem 279 55117 55126 10.1074/jbc.M404524200 (Pubitemid 40066505)
4. WM Bonner CE Redon JS Dickey AJ Nakamura OA Sedelnikova S Solier Y Pommier 2008 γH2AX and cancer Nat Rev Cancer 8 957 967 10.1038/nrc2523
5. Chronis F, Rogakou EP (2007) Interplay between γH2AX and 53BP1 pathways in DNA double strand break repair response. In: Gewirtz DA, Holt SE, Grant S (eds) Apoptosis, senescence, and cancer. 2nd edn. Humana Press, Totowa. pp 243-263
6. W Dirks M Wirth H Hauser 1993 Dicistronic transcription units for gene expression in mammalian cells Gene 128 247 249 10.1016/0378-1119(93)90569-O (Pubitemid 23189848)
7. Du G (2010) Focipicker3D, ImageJ plugin, http://rsbweb.nih.gov/ij/ plugins/foci-picker3d/index.html
8. B Elliott M Jasin 2001 Repair of double-strand breaks by homologous recombination in mismatch repair-defective mammalian cells Mol Cell Biol 21 2671 2682 10.1128/MCB.21.8.2671-2682.2001 (Pubitemid 32244912)
9. Gewirtz, DA, Holt, SE, Grant, S (eds) (2007) Apoptosis, senescence, and cancer. 2nd edn. Humana Press, Totowa
10. KD Greif HJ Brede D Frankenberg U Giesen 2004 The PTB single ion microbeam for irradiation of living cells Nucl Instrum Meth B 217 505 512 2004NIMPB.217..505G 10.1016/j.nimb.2003.11.082
11. KD Greif W Beverung F Langner D Frankenberg A Gellhaus F Banaz-Yasar 2006 The PTB microbeam: a versatile intrument for radiobiological research Radiat Prot Dosim 122 313 315 10.1093/rpd/ncl436 (Pubitemid 47090472)
12. Hable V (2010) Private communication
13. Z Hong J Jiang K Hashiguchi M Hoshi L Lan A Yasui 2008 Recruitment of mismatch repair proteins to the site of DNA damage in human cells J Cell Sci 121 3146 3154 10.1242/jcs.026393
14. B Jakob JH Rudolph N Gueven MF Lavin G Taucher-Scholz 2005 Live cell imaging of heavy-ion-induced radiation responses by beamline microscopy Radiat Res 163 681 690 10.1667/RR3374 (Pubitemid 40734767)
15. James F, Moneta L, Winkler M, Zsenei A (2008) Last MINUIT release. http://lcgapp.cern.ch/project/cls/work-packages/mathlibs/minuit/home.html
16. KK Khanna SP Jackson 2001 DNA double-strand breaks: signaling, repair and the cancer connection Nat Genet 27 247 254 10.1038/85798 (Pubitemid 32201842)
17. C Lukas F Melander M Stucki F Jacob S Bekker-Jensen M Goldberg Y Lerenthal SP jackson J Bartek J Lukas 2004 Mdc1 couples DNA double-strand break recognition by Nbs1 with its H2AX-dependent chromatin retention EMBO J 23 2674 2683 10.1038/sj.emboj.7600269 (Pubitemid 38988238)
18. M Lusizik-Bhadra F d'Errico L Lusini B Wiegel 1996 Microdosimetric investigations in a proton therapy beam with sequentially etched CR-39 track detectors Radiat Prot Dosim 66 353 358 (Pubitemid 126446911)
19. C Mielke M Tümmlerer D Schübeler I von Hoegen H Hause 2000 Stabilized, long-term expression of heterodimeric proteins from tricistronic mRNA Gene 254 1 8 10.1016/S0378-1119(00)00294-8
20. TT Paull M Gellert 1998 The 3' to 5' exonuclease activity of MRE11 facilitates repair of DNA double-strand breaks Mol Cel 1 969 979 10.1016/S1097-2765(00)80097-0 (Pubitemid 128379258)
21. R Rai H Dai AS Multani K Li K Chin J Gray JP Lahad J Liang GB Mills FM Bernstam S-Y Lin 2006 BRIT1 regulates early DNA damage response, chromosomal integrity, and cancer Cancer Cell 10 145 157 10.1016/j.ccr.2006.07.002 (Pubitemid 44175904)
22. EP Rogakou DR Pilch AH Orr VS Ivanova WM Bonner 1998 DNA Double-stranded breaks induce Histone H2AX Phosphorylation on Serine 139 J Biol Chem 273 5858 5868 10.1074/jbc.273.10.5858 (Pubitemid 28124064)
23. LB Schultz NH Chehab A Malikzay TD Halazonetis 2000 P53 binding protein 1 (53bp1) is an early participant in the cellular response to DNA double-strand breaks J Cell Biol 151 1381 1390 10.1083/jcb.151.7.1381 (Pubitemid 32047588)
24. D Simsek A Furda Y Gao J Artus E Brunet AK Hadjantonakis B Van Houten S Shuman PJ McKinnon M Jasin 2011 Crucial role for DNA ligase III in mitochondria but not in Xrcc1-dependent repair Nature 471 7337 245 248 2011Natur.471..245S 10.1038/nature09794
25. R Ugenskiene K Prise M Folkard J Lekki Z Stachura M Zazula J Stachura 2009 Dose response and kinetics of foci disappearance following exposure to high- and low-LET ionizing radiation Int J Radiat Biol 85 872 882
26. M Wang W Wu W Wu B Rosidi L Zhang H Wang G Iliakis 2006 PARP1 and Ku compete for repair of DNA double strand breaks by distinct NHEJ pathways Nucleic Acids Res 34 6170 6182 10.1093/nar/gkl840 (Pubitemid 44942738)