Genotoxicity evaluation of dentine bonding agents by comet assay

International Endodontic Journal

Volumn 44, Issue 9, 2011, Pages 807-816

  Kaya, A ^a   Ündeǧer, Ü ^b   Aydin, S ^b   Omurlu H ^a   Basaran N ^b  

^a GAZI UNIVERSITY   (Turkey)

^b HACETTEPE UNIVERSITY   (Turkey)

Author keywords

Clearfil Protect Bond; Clearfil SE Bond; Comet assay; Dentine bonding agents; DNA damage; I Bond; SL Bond

Indexed keywords

CLEARFIL PROTECT BOND; CLEARFIL SE BOND; DENTIN BONDING AGENT; IBOND; RESIN CEMENT;

ADULT; ANALYSIS OF VARIANCE; ARTICLE; CELL SURVIVAL; COMET ASSAY; COMPARATIVE STUDY; DENTAL BONDING; DNA DAMAGE; DOSE RESPONSE; DRUG EFFECT; FEMALE; HUMAN; LYMPHOCYTE;

ADULT; ANALYSIS OF VARIANCE; CELL SURVIVAL; COMET ASSAY; DENTAL BONDING; DENTIN-BONDING AGENTS; DNA DAMAGE; DOSE-RESPONSE RELATIONSHIP, DRUG; FEMALE; HUMANS; LYMPHOCYTES; RESIN CEMENTS;

EID: 79961031988     PISSN: 01432885     EISSN: 13652591     Source Type: Journal    
DOI: 10.1111/j.1365-2591.2011.01884.x     Document Type: Article

References (52)

1. Akimoto N, Momo Y, Kohno A, Suzuki S, Otsuki M, Cox CF (1998) Biocompatibility of Clearfil Liner Bond 2 and Clearfil AP-X system on non-exposed primate teeth. Quintessence International 29, 177-88.
2. Al-Hiyasat AS, Darmani H, Milhem MN (2005) Cytotoxicity evaluation of dental resin composites and their flowable derivatives. Clinical Oral Investigations 9, 21-5.
3. Arossi GA, Dihl RR, Lehmann M, Cunha KS, Reguly ML, de Andrade HHR (2009) In vivo genotoxicity of dental bonding agents. Mutagenesis 24, 169-72.
4. Bakopoulou AA, Triviai IN, Tsiftsoglou AS, Garefis PD (2006) In vitro assessment of cytotoxicty of resin-based dental restorative materials on WEHI 13 var fibroblasts. International Journal of Prosthodontics 19, 13-6.
5. Becher R, Kopperud HM, Al RH et al. (2005) Pattern of cell death after in vitro exposure to GDMA, TEGDMA, HEMA and two compomer extracts. Dental Materials 22, 630-40.
6. Bouillaguet S, Virgillito M, Wataha J, Ciucchi B, Holz J (1998) The influence of dentine permeability on cytotoxicity of four dentine bonding systems in vitro. Journal of Oral Rehabilitation 25, 45-51.
7. Boyum A (1976) Isolation of lymphocytes, granulocytes, and macrophages. Scandinavian Journal of Immunology 5, 9-15.
8. Brzovic V, Miletic I, Zeljezic D et al. (2009) In vitro genotoxicity of root canal sealers. International Endodontic Journal 42, 253-63.
9. Cao T, Saw TY, Heng BC, Liu H, Yap AU, Ng ML (2005) Comparison of different test models for the assessment of cytotoxicity of composite resins. Journal of Applied Toxicology 25, 101-8.
10. Cavalcanti BN, Rode SM, Marques MM (2005) Cytotoxicity of substances leached or dissolved from pulp capping materials. International Endodontic Journal 38, 505-9.
11. Cehreli ZC, Turgut M, Olmez S, Dagdeviren A, Atilla P (2000) Short term human primary pulpal response after direct pulp capping with fourth-generation dentine adhesives. Journal of Clinical Pediatric Dentistry 25, 65-71.
12. Chang HH, Guo MK, Kasten FH et al. (2005) Stimulation of glutathione depletion, ROS production and cell cycle arrest of dental pulp cells and gingival epithelial cells by HEMA. Biomaterials 26, 745-53.
13. Cimpan MR, Matre R, Skaug N, Lie SA, Lygre H (2005) The coinitiator DMABEE induces death by apoptosis induces death by apoptosis and necrosis in human monoblastoid cells. Clinical Oral Investigations 9, 168-72.
14. Collins AR, Dobson VL, Dusinka M, Kennedy G, Stetina R (1997) The comet assay: what can it really tell us? Mutation Research 375, 183-93.
15. Costa CAS, Teixeira HM, Nascimento L, Batista A, Hebling J (2000) Biocompatibility of two current adhesive resins. Journal of Endodontics 26, 512-6.
16. Cox CF, Hafez AA, Akimoto N, Otsuki M, Suzuki S, Tarim B (1998) Biocompatibility of primer, adhesive and resin composite systems on non-exposed and exposed pulps of non-human primate teeth surface. American Journal of Dentistry 10, 55-63.
17. Cross NG, Taylor RF, Nunez L (1983) Single-step orthodontic bonding system: possible mutagenic potential. American Journal of Orthodontics 84, 345-50.
18. Demirci H, Hiller KA, Bosl C, Galler K, Schmalz G, Schwekl H (2008) The induction of oxidative stress, cytotoxicity, and genotoxicity by dental adhesives. Dental Materials 24, 362-71.
19. Dhawan A, Anderson D (2009) The comet assay in toxicology. Cambridge, UK: The Royal Society of Chemistry.
20. Fredericks HF (1981) Mutagenic potential of orthodontic bonding materials. American Journal of Orthodontics 80, 316-24.
21. Gerzina TM, Hume WR (1994) Effect of dentine on release of TEGDMA from resin composite in vitro. Journal of Oral Rehabilitation 21, 463-8.
22. Geurtsen W (2000) Biocompatibility of resin-modified filling materials. Critical Reviews in Oral Biology & Medicine 11, 333-55.
23. Geurtsen W (2001) Biocompatibility of root canal filling materials. Australian Endodontic Journal 27, 12-21.
24. Geurtsen W (2003) Toxicology of Dental Materials and 'Clinical Experience'. Journal of Dental Research 82, 500.
25. Goldberg M (2008) In vitro and in vivo studies on the toxicity of dental resin components: a review. Clinical Oral Investigations 12, 1-8.
26. Hamann CP, Rodgers PA, Sullivan KM (2004) Occupational allergens in dentistry. Current Opinion in Allergy and Clinical Immunology 4, 403-9.
27. Huang FM, Chang YC (2002) Cytotoxicity of dentine-bonding agents on human pulp cells in vitro. International Endodontic Journal 35, 905-9.
28. Huang FM, Chou MY, Chang YC (2003) Dentine bonding agents induce c-fos and c-jun protooncogenes expression in human gingival fibroblasts. Biomaterials 24, 157-63.
29. Isaksson M, Lindberg M, Sundberg K, Hallander A, Bruze M (2005) The development and course of patch-test reactions to 2-hydroxyethyl methacrylate and ethyleneglycol dimethacrylate. Contact Dermatitis 53, 292-7.
30. Janke V, von Neuhoff N, Schlegelberger B, Leyhausen G, Geurtsen W (2003) TEGDMA causes apoptosis in primary human gingival fibroblasts. Journal of Dental Research 82, 814-8.
31. Kleinsasser NH, Wallner BC, Harreus UA et al. (2004) Genotoxicity and cytotoxicity of dental materials in human lymphocytes as assessed by the single cell microgel electrophoresis (comet) assay. Journal of Dentistry 32, 229-34.
32. Kleinsasser NH, Schmid K, Sassen AW et al. (2006) Cytotoxic and genotoxic effects of resin monomers in human salivary gland tissue and lymphocytes as assessed by the single cell microgel electrophoresis (comet) assay. Biomaterials 27, 1762-70.
33. Miletic I, Anic I, Karlovic Z, Marsan T, Pezelj-Ribaric S, Omsak M (2000) Cytotoxic effect of four root filling materials. Endodontics and Dental Traumatology 16, 287-90.
34. Miller EG, Thompson LR, Zimmermann ER, Bowles WH (1984) In vivo studies on the carcinogenic potential of an orthodontic bonding resin. American Journal of Orthodontics 86, 342-6.
35. Müller BP, Eisenträger A, Jahnen-Dechent W, Dott W, Hollender J (2003) Effect of sample preparation on the in vitro genotoxicity of a light curable glass ionomer cement. Biomaterials 24, 611-7.
36. Noda M, Wataha JC, Kaga M, Lockwood PE, Volkmann KR, Sano H (2002) Components of dental adhesives modulate heat shock protein 72 expression in heat-stressed THP-1 human monocytes at sublethal concentrations. Journal of Dental Research 81, 265-9.
37. Ortengren U, Wellendorf H, Karlsson S, Ruyter IE (2001) Water sorption and solubility of dental composites and identification of monomers released in an aqueous environment. Journal of Oral Rehabilitation 28, 1106-15.
38. Peutzfeldt A (1997) Resin composites in dentistry: the monomer systems. European Journal of Oral Sciences 105, 97-116.
39. Prica D, Galic N, Zeljezic D, Prica A (2006) Genotoxicity evaluation of five different dentine bonding agents by chromosomal aberration analysis. Journal of Oral Rehabilitation 33, 462-71.
40. Reichl FX, Esters M, Simon S et al. (2006) Cell death effects of resin-based dental material compounds and materials in human gingival fibroblasts. Archives of Toxicology 80, 370-7.
41. Schedle A, Franz A, Rausch-Fan X et al. (1998) Cytotoxic effect of dental composites, adhesive substances, compomers and cements. Dental Materials 14, 429-40.
42. Schweikl H, Schmalz G, Spruss T (2001) The induction of micronuclei in vitro by unpolymerized resin monomers. Journal of Dental Research 80, 1615-20.
43. Schweikl H, Spagnuolo G, Schmalz G (2006) Genetic and cellular toxicology of dental resin monomers. Journal of Dental Research 85, 870-7.
44. Schwengberg S, Bohlen H, Kleinsasser N et al. (2005) In vitro embryotoxicity assessment with dental restorative materials. Journal of Dentistry 33, 49-55.
45. Singh NP, McCoy MT, Tice RR, Schneider EL (1988) A simple technique for quantitation of low levels of DNA damage in individual cells. Experimental Cell Research 175, 184-91.
46. Stanislawski L, Lefeuvre M, Bourd K, Soheili-Majd E, Goldberg M, Perianin A (2003) TEGDMA-induced toxicity in human fibroblasts is associated with early and drastic glutathione depletion with subsequent production of oxygen reactive species. Journal of Biomedical Materials Research 66, 476-82.
47. Strober W (2001) Trypan Blue Exclusion Test of Cell Viability. Current Protocols in Immunology A.3B.1-2.
48. Sutow EJ, Maillet WA, Hall GC (2006) Corrosion potential variation of aged dental amalgam restorations over time. Dental Materials 22, 325-9.
49. Szep S, Kunkel A, Ronge K, Heidemann D (2002) Cytotoxicity of modern dentine adhesives-in vitro testing on gingival fibroblasts. Journal of Biomedical Materials Research 63, 53-60.
50. Tosic G (2004) Occupational hazards in dentistry - part one: allergic reactions to dental restorative materials and latex sensitivity. Facta Universitatis 2, 317-24.
51. Valko M, Izakovic M, Mazur M, Rhodes CJ, Telser J (2006) Role of oxygen radicals in DNA damage and cancer incidence. Molecular and Cellular Biochemistry 266, 37-56.
52. Walther SC, Liebls B, Reichl FX, Kehe K, Nilius M, Hickel R (2002) Cytotoxicity of ingredients of various dental materials and related compounds in L2 and A549 cells. Journal of Biomedical Materials Research 63, 643-9.