In vitro genotoxicity of root canal sealers

International Endodontic Journal

Volumn 42, Issue 3, 2009, Pages 253-263

  Brzovic, V ^b   Miletic, I ^b   Zeljezic, D ^a   Mladinic, M ^a   Kasuba, V ^a   Ramic, S ^c   Anic, I ^b  

^a INSTITUTE FOR MEDICAL RESEARCH AND OCCUPATIONAL HEALTH   (Croatia)

^b UNIVERSITY OF ZAGREB   (Croatia)

^c SESTRE MILOSRDNICE UNIVERSITY HOSPITAL   (Croatia)

Author keywords

Chromosomal aberrations; Comet assay; Cytotoxicity; Genotoxicity; In vitro; Root canal sealers

Indexed keywords

BIOMATERIAL; BISMUTH; COLORING AGENT; DENTIN BONDING AGENT; DIAGNOSTIC AGENT; DIAKET; DIMETICONE; EPIPHANY SEALER; GUTTA PERCHA; GUTTAFLOW; HERMETIC; IRM CEMENT; METHACRYLIC ACID METHYL ESTER; MUTAGENIC AGENT; POLYVINYL DERIVATIVE; ROOT CANAL FILLING MATERIAL; SUPER EBA; TRYPAN BLUE; ZINC OXIDE; ZINC OXIDE EUGENOL;

ARTICLE; CELL CULTURE; CELL SURVIVAL; CHEMICALLY INDUCED DISORDER; CHROMATID; CHROMOSOME ABERRATION; CLASSIFICATION; COMET ASSAY; DRUG COMBINATION; DRUG EFFECT; HUMAN; LYMPHOCYTE; MATERIALS TESTING; TIME; TOOTH DISEASE;

BIOCOMPATIBLE MATERIALS; BISMUTH; CELL SURVIVAL; CELLS, CULTURED; CHROMATIDS; CHROMOSOME ABERRATIONS; COLORING AGENTS; COMET ASSAY; DENTAL LEAKAGE; DENTIN-BONDING AGENTS; DIMETHYLPOLYSILOXANES; DRUG COMBINATIONS; GUTTA-PERCHA; HUMANS; LYMPHOCYTES; MATERIALS TESTING; METHYLMETHACRYLATES; MUTAGENS; POLYVINYLS; ROOT CANAL FILLING MATERIALS; TIME FACTORS; TRYPAN BLUE; ZINC OXIDE; ZINC OXIDE-EUGENOL CEMENT;

EID: 59549107030     PISSN: 01432885     EISSN: 13652591     Source Type: Journal    
DOI: 10.1111/j.1365-2591.2008.01510.x     Document Type: Article

References (47)

1. Asrari M, Lobner D (2003) In vitro neurotoxic evaluation of root-end-filling materials. Journal of Endodontics 29, 743 6.
2. Bakopoulou A, Mourelatos D, Tsiftsoglou AS, Mioglou E, Garefis P (2008) Sister-chromatid exchange, chromosomal aberrations and delays in cell-cycle kinetics in human lymphocytes induced by dental composite resin eluates. Mutation Research 649, 79 90.
3. Bogdanffy MS, Valentine R (2003) Differentiating between local cytotoxicity, mitogenesis, and genotoxicity in carcinogen risk assessments: the case of vinyl acetate. Toxicology Letters 11, 83 98.
4. Briseno BM, Willershausen B (1991) Root canal sealer cytotoxicity on human gingival fibroblasts: 2. Silicone- and resin-based sealers. Journal of Endodontics 17, 537 40.
5. 2+ions into synthetic saliva from an high-copper dental amalgam. Journal of Materials Science: Materials in Medicine 18, 1521 7.
6. Chang YC, Tai KW, Huang FM, Huang MF (2000) Cytotoxic and nongenotoxic effects of phenolic compounds in human pulp cell cultures. Journal of Endodontics 26, 440 3.
7. Collins AR (2004) The Comet Assay for DNA damage and repair: principles, applications, and limitations. Molecular Biotechnology 26, 249 61.
8. Czarnecka B, Limanowska-Shaw H, Nicholson JW (2003) Ion-release, dissolution and buffering by zinc phosphate dental cements. Journal of Materials Science: Materials in Medicine 14, 601 4.
9. Demirci M, Hiller K-A, Bosl C, Galler K, Schmalz G, Schweikl H (2008) The induction of oxidative stress, cytotoxicity, and genotoxicity by dental adhesives. Dental Materials 24, 362 71.
10. Dufour EK, Kumaravel T, Nohynek GJ, Kirkland D, Toutain H (2006) Clastogenicity, photo-clastogenicity or pseudo-photo-clastogenicity: Genotoxic effects of zinc oxide in the dark, in pre-irradiated or simultaneously irradiated chinese hamster ovary cells. Mutation Research 607, 215 24.
11. Eldeniz AU, Erdemir A, Kurtoglu F, Esener T (2007a) Evaluation of pH and calcium ion release of Acroseal sealer in comparison with Apexit and Sealapex sealers. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics 103, e86 91.
12. Eldeniz AU, Mustafa K, Ørstavik D, Dahl JE (2007b) Cytotoxicity of new resin-, calcium hydroxide- and silicone-based root canal sealers on fibroblasts derived from human gingiva and L929 cell lines. International Endodontic Journal 40, 329 37.
13. Ellahuene MF, Perez-Alzola LP, Orellana-Valdebenito M, Munoz C, Lafuente-Indo N (1994) Genotoxic evaluation of eugenol using the bone marrow micronucleus assay. Mutation Research 320, 175 80.
14. Fujisawa S, Masuhara E (1979) The in vitro biological evaluation of dental cements using elution and binding by bovine serum albumin. Shika Rikogaku Zasshi (Journal of the Japan Society for Dental Apparatus and Materials) 20, 142 50.
15. Geurtsen W (1998) Substances releases from non-amalgam dental filling materials. European Journal of Oral Sciences 106, 687 95.
16. Geurtsen W (2000) Biocompatibility of resin-modified filling materials. Critical Reviews in Oral Biology and Medicine 11, 333 55.
17. Geurtsen W, Leyhausen G (1997) Biological aspects of root canal filling materials-histocompatibility, cytotoxicity, and mutagenicity. Clinical Oral Investigations 1, 5 11.
18. Gulati N, Chandra S, Aggarwal PK, Jaiswal JN, Singh M (1991) Cytotoxicity of eugenol in sealer containing zinc oxide. Endodontics and Dental Traumatology 7, 181 5.
19. Hagiwara M, Watanabe E, Barrett JC, Tsutsui T (2006) Assessment of genotoxicity of 14 chemical agents used in dental practice: ability to induce chromosome aberrations in Syrian hamster embryo cells. Mutation Research 603, 111 20.
20. Heil J, Reifferscheid G, Waldmann P, Leyhausen G, Geurtsen W (1996) Genotoxicity of dental materials. Mutation Research 28, 181 94.
21. Hikiba H, Watanabe E, Carl Barrett J, Tsutsui T (2005) Ability of fourteen chemical agents used in dental practice to induce chromosome aberrations in syrian hamster embryo cells. Journal of Pharmaceutical Sciences 97, 146 52.
22. Huang TS, Lee H, Kao CT (2001) Evaluation of the genotoxicity of zinc oxide eugenol-based, calcium hydroxide-based, and epoxy resin-based root canal sealers by comet assay. Journal of Endodontics 27, 744 8.
23. Huang FM, Chou MY, Chang YC (2003) Dentin bonding agents induce c-fos and c-jun protooncogenes expression in human gingival fibroblasts. Biomaterials 24, 157 63.
24. Imazato S, Horikawa D, Nishida M, Ebisu S (2009) Effects of monomers eluted from dental resin restoratives on osteoblast-like cells. Journal of Biomedical Materials Research. Part B, Applied Biomaterials, In Press.
25. International Atomic Energy Agency IAEA (2001) Cytogenetic Analysis for Radiation Dose Assessment. A Manual TechRepSer No 405. Vienna, Austria : International Atomic Energy Agency.
26. Kolokuris I, Katsaki-Kovatsi VP, Economides N, Pouloloulos A, Razos G, Vlemmas I (1998) Influence of zinc oxide and eugenol sealer on concentration of zinc, calcium and copper in rat tissues. Endodontics and Dental Traumatology 14, 210 3.
27. Maura A, Pino A, Ricci R (1989) Negative evidence in vivo of DNA-damaging, mutagenic and chromosomal effects of eugenol. Mutation Research 227, 125 9.
28. Meryon SD, Brook AM (1990) In vitro comparison of the cytotoxicity of twelve endodontic materials using a new technique. International Endodontic Journal 23, 203 10.
29. Miletic I, Anic I, Karlovic Z, Marsan T, Pezelj-Ribaric S, Osmak M (2000) Cytotoxic effect of four root filling materials. Endodontics and Dental Traumatology 16, 287 90.
30. Nencka D, Walia HD, Austin BP (1995) Histologic evaluation of the biocompatibilty of Diaket. Journal of Dental Research 74, 101.
31. Newell DG (1998) Trypan blue exclusion method for cell viability estimation. In : Doyle A, Griffiths JB, eds. Cell and Tissue Culture: Laboratory Procedures. London, UK : John Wiley and Sons Ltd, pp. 77 82.
32. Nishimura H, Higo Y, Ohno M, Tsutsui TW, Tsutsui T (2008) Ability of root canal antiseptics used in dental practice to induce chromosome aberrations in human dental pulp cells. Mutation Research 649, 45 53.
33. Obe G, Pfeiffer P, Savage JRK et al. (2005) Chromosomal aberrations: formation, identification and distribution. Mutation Research 504, 17 36.
34. Olsson B, Siwkowski A, Langeland K (1981) Intraosseus implantation for biological evaluation of endodontic materials. Journal of Endodontics 7, 253 65.
35. Organisation for Economic Cooperation and Development. OECD (1997) Genetic Toxicology: In Vitro Mammalian Cytogenetic Test Guidelines for the Testing of Chemicals No. 473 Paris, France : OECD Publishing.
36. Ørstavik D (2005) Materials used for root canal obturation: technical, biological and clinical testing. Endodontic Topics 12, 25 38.
37. Prica D, Galic N, Zeljezic D, Prica A (2006) Genotoxicity evaluation of five different dentin bonding agents by chromosomal aberration analysis. Journal of Oral Rehabilitation 33, 462 71.
38. Ribeiro DA (2008) Do endodontic compounds induce genetic damage? A comprehensive review. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics 105, 251 6.
39. Schweikl H, Schmalz G, Spruss T (2001) The induction of micronuclei in vitro by unpolymerized resin monomers. Journal of Dental Research 80, 1615 20.
40. Serene TP, Vesely J, Boackle RJ (1988) Complement activation as a possible in vitro indication of the inflammatory potential of endodontic materials. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics 65, 354 7.
41. Singh NP, McCoy MT, Tice RR, Schneider EL (1988) A simple technique for quantitation of low levels of DNA damage in individual cells. Experimental Cell Research 175, 184 91.
42. Spahl W, Budzikiewicz H (1994) Qualitative analysis of dental resin composites by gas and liquid chromatographyhass spectrometry. Fresenius Journal of Analytical Chemistry 350, 684 91.
43. Spangberg L (1969) Biological effects of root-canal-filling materials. Part 4. Effect in vitro of water-soluble components of root-canal-filling materials on HeLa cells. Odontologisk Revy 20, 289 99.
44. Stanley HR (1992) Local and systemic responses to dental composites and glass ionomers. Advances in Dental Research 6, 55 64.
45. Tai KW, Huang FM, Huang MS, Chang YC (2001) Assessment of the genotoxicity of resin and zinc-oxide eugenol-based root canal sealers using an in vitro mammalian test system. Journal of Biomedical Materials Research 59, 73 7.
46. Takahara K, Onodera A, Matsumoto K (1990) Toxicity of root canal sealers on rat bone cells in primary culture. Endodontics and Dental Traumatology 6, 200 7.
47. Torabinejad M, Hong CU, Pitt Ford TR, Kettering JD (1995) Cytotoxicity of four root end filling materials. Journal of Endodontics 21, 489 92.