Genetically encoding unnatural amino acids in neural stem cells and optically reporting voltage-sensitive domain changes in differentiated neurons

Stem Cells

Volumn 29, Issue 8, 2011, Pages 1231-1240

  Shen, Bin ^a   Xiang, Zheng ^a   Miller, Barbara ^a   Louie, Gordon ^a   Wang, Wenyuan ^a   Noel, Joseph P ^a,b   Gage, Fred H ^a   Wang, Lei ^a  

^a SALK INSTITUTE FOR BIOLOGICAL STUDIES   (United States)

^b HOWARD HUGHES MEDICAL INSTITUTE   (United States)

Author keywords

Fluorescence imaging; Neural stem cells; Unnatural amino acids; Voltage sensing

Indexed keywords

AMINO ACID; AMINOACYL TRANSFER RNA; GREEN FLUORESCENT PROTEIN; LENTIVIRUS VECTOR; PHOSPHATIDATE PHOSPHATASE; TRANSFER RNA; UNCLASSIFIED DRUG; UNNATURAL AMINO ACID;

ANIMAL CELL; ARTICLE; CELL DIFFERENTIATION; CONFORMATIONAL TRANSITION; GENETIC TRANSFECTION; MEMBRANE DEPOLARIZATION; MEMBRANE POTENTIAL; NERVE CELL; NERVOUS SYSTEM DEVELOPMENT; NEURAL STEM CELL; NONHUMAN; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; RAT;

ALANINE; AMINO ACYL-TRNA SYNTHETASES; ANIMALS; BENZOPHENONES; CELL DIFFERENTIATION; CELL LINE; DANSYL COMPOUNDS; FLUORESCENCE; GENETIC ENGINEERING; GENETIC VECTORS; HUMANS; LENTIVIRUS; MEMBRANE POTENTIALS; NEURAL STEM CELLS; NEURONS; PATCH-CLAMP TECHNIQUES; PHENYLALANINE; PHOSPHORIC MONOESTER HYDROLASES; PROTEIN CONFORMATION; PROTEIN STRUCTURE, TERTIARY; RATS; TIME-LAPSE IMAGING; TYROSINE-TRNA LIGASE;

BACTERIA (MICROORGANISMS); MAMMALIA;

EID: 79960877220     PISSN: 10665099     EISSN: None     Source Type: Journal    
DOI: 10.1002/stem.679     Document Type: Article

References (40)

1. Wang L Schultz PG. Expanding the genetic code. Angew Chem Int Ed Engl 2005 44:34-66.
2. Wang Q, Parrish AR, Wang L. Expanding the genetic code for biological studies. Chem Biol 2009;16:323-336.
3. Wang L, Brock A, Herberich B et al. Expanding the genetic code of Escherichia coli. Science 2001;292:498-500.
4. Wang Q, Wang L. New methods enabling efficient incorporation of unnatural amino acids in yeast. J Am Chem Soc 2008;130:6066-6067.
5. Sakamoto K, Hayashi A, Sakamoto A et al. Site-specific incorporation of an unnatural amino acid into proteins in mammalian cells. Nucleic Acids Res 2002;30:4692-4699.
6. Liu W, Brock A, Chen S et al. Genetic incorporation of unnatural amino acids into proteins in mammalian cells. Nat Methods 2007;4:239-244.
7. Wang W, Takimoto JK, Louie GV et al. Genetically encoding unnatural amino acids for cellular and neuronal studies. Nat Neurosci 2007;10:1063-1072.
8. Murry CE, Keller G. Differentiation of embryonic stem cells to clinically relevant populations: Lessons from embryonic development. Cell 2008;132:661-680.
9. Rossi DJ, Jamieson CH, Weissman IL. Stems cells and the pathways to aging and cancer. Cell 2008;132:681-696.
10. Ma DK, Marchetto MC, Guo JU et al. Epigenetic choreographers of neurogenesis in the adult mammalian brain. Nat Neurosci 2010;13:1338-1344.
11. Murata Y, Iwasaki H, Sasaki M et al. Phosphoinositide phosphatase activity coupled to an intrinsic voltage sensor. Nature 2005;435:1239-1243.
12. Gage FH, Coates PW, Palmer TD et al. Survival and differentiation of adult neuronal progenitor cells transplanted to the adult brain. Proc Natl Acad Sci USA 1995;92:11879-11883.
13. Hamill OP, Marty A, Neher E et al. Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflugers Arch 1981;391:85-100.
14. Zhao C, Deng W, Gage FH. Mechanisms and functional implications of adult neurogenesis. Cell 2008;132:645-660.
15. Holst J, Vignali KM, Burton AR et al. Rapid analysis of T-cell selection in vivo using T cell-receptor retrogenic mice. Nat Methods 2006;3:191-197.
16. Takimoto JK, Adams KL, Xiang Z et al. Improving orthogonal tRNA-synthetase recognition for efficient unnatural amino acid incorporation and application in mammalian cells. Mol Biosyst 2009;5:931-934.
17. Summerer D, Chen S, Wu N et al. A genetically encoded fluorescent amino acid. Proc Natl Acad Sci USA 2006;103:9785-9789.
18. Kohout SC, Ulbrich MH, Bell SC et al. Subunit organization and functional transitions in Ci-VSP. Nat Struct Mol Biol 2008;15:106-108.
19. Wu S, Zhang Y. LOMETS: A local meta-threading-server for protein structure prediction. Nucleic Acids Res 2007;35:3375-3382.
20. Chen X, Wang Q, Ni F et al. Structure of the full-length Shaker potassium channel Kv1.2 by normal-mode-based X-ray crystallographic refinement. Proc Natl Acad Sci USA 2010;107:11352-11357.
21. + channel in a lipid membrane-like environment. Nature 2007;450:376-382.
22. Dimitrov D, He Y, Mutoh H et al. Engineering and characterization of an enhanced fluorescent protein voltage sensor. PLoS One 2007;2:e440.
23. Villalba-Galea CA, Sandtner W, Starace DM et al. S4-based voltage sensors have three major conformations. Proc Natl Acad Sci USA 2008;105:17600-17607.
24. Lundby A, Mutoh H, Dimitrov D et al. Engineering of a genetically encodable fluorescent voltage sensor exploiting fast Ci-VSP voltagesensing movements. PLoS One 2008;3:e2514.
25. Brun S, Faucon-Biguet N, Mallet J. Optimization of transgene expression at the posttranscriptional level in neural cells: Implications for gene therapy. Mol Ther 2003;7:782-789.
26. ter Brake O, t Hooft K, Liu YP et al. Lentiviral vector design for multiple shRNA expression and durable HIV-1 inhibition. Mol Ther 2008;16:557-564.
27. Hong S, Hwang DY, Yoon S et al. Functional analysis of various promoters in lentiviral vectors at different stages of in vitro differentiation of mouse embryonic stem cells. Mol Ther 2007;15:1630-1639.
28. Mannuzzu LM, Moronne MM, Isacoff EY. Direct physical measure of conformational rearrangement underlying potassium channel gating. Science 1996;271:213-216.
29. + channel with fluorescence. Neuron 1997;19:1127-1140.
30. Glauner KS, Mannuzzu LM, Gandhi CS et al. Spectroscopic mapping of voltage sensor movement in the Shaker potassium channel. Nature 1999;402:813-817.
31. Smith PL, Yellen G. Fast and slow voltage sensor movements in HERG potassium channels. J Gen Physiol 2002;119:275-293.
32. Zheng J, Zagotta WN. Gating rearrangements in cyclic nucleotide-gated channels revealed by patch-clamp fluorometry. Neuron 2000;28:369-374.
33. Dahan DS, Dibas MI, Petersson EJ et al. A fluorophore attached to nicotinic acetylcholine receptor beta M2 detects productive binding of agonist to the alpha delta site. Proc Natl Acad Sci USA 2004;101:10195-10200.
34. + channel, revealed by voltage-clamp fluorometry. Proc Natl Acad Sci USA 2006;103:12619-12624.
35. Bezanilla F. Ion channels: From conductance to structure. Neuron 2008;60:456-468.
36. + channel. Nature 2003;423:42-48.
37. Ahern CA, Horn R. Focused electric field across the voltage sensor of potassium channels. Neuron 2005;48:25-29.
38. +channel voltage sensor measured with luminescence energy transfer. Nature 2005;436:848-851.
39. Chanda B, Asamoah OK, Blunck R et al. Gating charge displacement in voltage-gated ion channels involves limited transmembrane movement. Nature 2005;436:852-856.
40. Swartz KJ. Sensing voltage across lipid membranes. Nature 2008;456:891-897.