A fluorophore attached to nicotinic acetylcholine receptor βM2 detects productive binding of agonist to the αδ site

Proceedings of the National Academy of Sciences of the United States of America

Volumn 101, Issue 27, 2004, Pages 10195-10200

  Dahan, David S ^a   Dibas, Mohammed I ^b   Petersson, E James ^a   Auyeung, Vincent C ^b   Chanda, Baron ^c   Bezanilla, Francisco ^c   Dougherty, Dennis A ^a   Lester, Henry A ^b  

^a California Institute of Technology   (United States)

^b CALIFORNIA INSTITUTE OF TECHNOLOGY   (United States)

^c UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACETYLCHOLINE; CYSTEINE; EPIBATIDINE; FLUORESCENT DYE; NICOTINIC RECEPTOR; RHODAMINE;

ANIMAL CELL; ARTICLE; BINDING AFFINITY; BINDING SITE; CONCENTRATION RESPONSE; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; DESENSITIZATION; FLUORESCENCE; HYDROPHOBICITY; NONHUMAN; PRIORITY JOURNAL; RECEPTOR BINDING; RECEPTOR INTRINSIC ACTIVITY; XENOPUS;

ACETYLCHOLINE; ANIMALS; BICYCLO COMPOUNDS, HETEROCYCLIC; BINDING SITES; FEMALE; FLUORESCENCE; FLUORESCENT DYES; HYDROPHOBICITY; ION CHANNEL GATING; NICOTINIC AGONISTS; PROTEIN CONFORMATION; PROTEIN SUBUNITS; PYRIDINES; RECEPTORS, NICOTINIC; XENOPUS;

ANIMALIA;

EID: 3042829624     PISSN: 00278424     EISSN: None     Source Type: Journal    
DOI: 10.1073/pnas.0301885101     Document Type: Article

References (26)

1. Miyazawa, A., Fujiyoshi, Y., Stowell, M. & Unwin, N. (1999) J. Mol. Biol. 288, 765-786.
2. Karlin, A., Holtzman, E., Yodh, N., Lobel, P., Wall, J. & Hainfeld, J. (1983) J. Biol. Chem. 258, 6678-6681.
3. Brejc, K., van Dijk, W. J., Klaassen, R. V., Schuurmans, M., van Der Oost, J., Smit, A. B. & Sixma, T. K. (2001) Nature 411, 269-276.
4. Miyazawa, A., Fujiyoshi, Y. & Unwin, N. (2003) Nature 424, 949-955.
5. Karlin, A. (2002) Nat. Rev. Neurosci. 3, 102-114.
6. Changeux, J. P. & Edelstein, S. J. (1998) Neuron 21, 959-980.
7. Katz, B. & Thesleff, S. (1957) J. Physiol. (London) 138, 63-80.
8. Zhang, Y., Chen, J. & Auerbach, A. (1995) J. Physiol. (London) 486, 189-206.
9. Blount, P. & Merlie, J. P. (1989) Neuron 3, 349-357.
10. Prince, R. J. & Sine, S. M. (1998) Biophys. J. 75, 1817-1827.
11. Sine, S. M. & Claudio, T. (1991) J. Biol. Chem. 266, 19369-19377.
12. Chang, Y. & Weiss, D. S. (2002) Nat. Neurosci. 5, 1163-1168.
13. Nerbonne, J. M., Sheridan, R. E., Chabala, L. D. & Lester, H. A. (1983) Mol. Pharmacol. 23, 344-349.
14. Quick, M. & Lester, H. A. (1994) in Ion Channels of Excitable Cells, ed. Narahashi, T. (Academic, San Diego), pp. 261-279.
15. Zhang, H. & Karlin, A. (1998) Biochemistry 37, 7952-7964.
16. Cha, A. & Bezanilla, F. (1998) J. Gen. Physiol. 112, 391-408.
17. Li, M., Farley, R. A. & Lester, H. A. (2000) J. Gen. Physiol. 115, 491-508.
18. Hille, B. (2001) Ion Channels of Excitable Membranes (Sinauer, Sunderland, MA).
19. Lester, H. A. & Nerbonne, J. M. (1982) Annu. Rev. Biophys. Bioeng. 11, 151-175.
20. Rang, H. P. & Ritter, J. M. (1970) Mol. Pharmacol. 6, 357-382.
21. Chang, Y., Ghansah, E., Chen, Y., Ye, J. & Weiss, D. S. (2002) J. Neurosci. 22, 7982-7990.
22. Sine, S. M., Ohno, K., Bouzat, C., Auerbach, A., Milone, M., Pruitt, J. N. & Engel, A. G. (1995) Neuron 15, 229-239.
23. Unwin, N. (2003) FEBS Lett. 555, 91-95.
24. Jackson, M. B. (1994) Trends Biochem. Sci. 19, 396-399.
25. Karpen, J. W. & Ruiz, M. (2002) Trends Biochem. Sci. 27, 402-409.
26. Koshland, D. E., Jr., Nemethy, G. & Filmer, D. (1966) Biochemistry 5, 365-385.