Simvastatin induces apoptosis by a Rho-dependent mechanism in cultured cardiac fibroblasts and myofibroblasts

Toxicology and Applied Pharmacology

Volumn 255, Issue 1, 2011, Pages 57-64

  Copaja, Miguel ^a   Venegas, Daniel ^a   Aránguiz, Pablo ^a   Canales, Jimena ^a   Vivar, Raúl ^a   Catalán, Mabel ^a   Olmedo, Ivonne ^a   Rodríguez, Andrea E ^a   Chiong, Mario ^a   Leyton, Lisette ^a,b   Lavandero, Sergio ^a,b   Díaz Araya, Guillermo ^a  

^a UNIVERSITY OF CHILE   (Chile)

^b UNIVERSITY OF CHILE   (Chile)

Author keywords

Apoptosis; Cell death; Fibroblast; Heart; HMG coA reductase; Myofibroblast; Rho A; Statin

Indexed keywords

CASPASE 3; DNA; FARNESYL DIPHOSPHATE; GERANYLGERANYL PYROPHOSPHATE; MEVALONIC ACID; RHO FACTOR; RHOA GUANINE NUCLEOTIDE BINDING PROTEIN; SIMVASTATIN; SQUALENE; TRYPAN BLUE;

ANIMAL CELL; APOPTOSIS; ARTICLE; CARDIAC FIBROBLAST; CELL TYPE; CELL VIABILITY; CONCENTRATION RESPONSE; CONTROLLED STUDY; DNA FRAGMENTATION; DRUG MECHANISM; ENZYME ACTIVATION; FIBROBLAST; FLOW CYTOMETRY; MYOFIBROBLAST; NEWBORN; NONHUMAN; RAT; WESTERN BLOTTING;

ANIMALS; APOPTOSIS; BENZAMIDES; CASPASES; CELL SURVIVAL; CELLS, CULTURED; CHOLESTEROL; FIBROBLASTS; HEART; HYDROXYMETHYLGLUTARYL-COA REDUCTASE INHIBITORS; METHIONINE; MYOFIBROBLASTS; RATS; RATS, SPRAGUE-DAWLEY; RHOA GTP-BINDING PROTEIN; SIMVASTATIN;

RATTUS;

EID: 79960834633     PISSN: 0041008X     EISSN: 10960333     Source Type: Journal    
DOI: 10.1016/j.taap.2011.05.016     Document Type: Article

References (38)

1. Arora P.D., McCulloch C,.A. The deletion of transforming growth factor-beta-induced myofibroblasts depends on growth conditions and actin organization. Am. J. Pathol. 1999, 155:2087-2099.
2. Banerjee I., Fuseler J.W., Price R.L., Borg T.K., Baudino T.A. Determination of cell types and numbers during cardiac development in the neonatal and adult rat and mouse. Am. J. Physiol. 2007, 293:H1883-H1891.
3. + exchanger. FEBS Lett. 1994, 343:151-154.
4. Blanco-Colio L.M., Villa A., Ortego M., Hernandez-Presa M.A., Pascual A., Plaza J.J., Egido J. 3-Hydroxy-3-methyl-glutaryl coenzyme A reductase inhibitors, atorvastatin and simvastatin, induce apoptosis of vascular smooth muscle cells by downregulation of Bcl-2 expression and Rho A prenylation. Atherosclerosis 2002, 161:17-26.
5. Brown R.D., Ambler S.K., Mitchell M.D., Long C.S. The cardiac fibroblast: therapeutic target in myocardial remodeling and failure. Annu. Rev. Pharmacol. Toxicol. 2005, 45:657-687.
6. Chen S., Crawford M., Day R.M., Briones V.R., Leader J.E., Jose P.A., Lechleider R.J. RhoA modulates Smad signaling during transforming growth factor-beta-induced smooth muscle differentiation. J. Biol. Chem. 2006, 281:1765-1770.
7. Dandapat A., Hu C.P., Li D., Liu Y., Chen H., Hermonat P.L., Mehta J.L. Overexpression of TGFbeta1 by adeno-associated virus type-2 vector protects myocardium from ischemia-reperfusion injury. Gene Ther. 2008, 6:415-423.
8. Demyanets S., Kaun C., Pfaffenberger S., Hohensinner P.J., Rega G., Pammer J., Maurer G., Huber K., Wojta J. Hydroxymethylglutaryl-coenzyme A reductase inhibitors induce apoptosis in human cardiac myocytes in vitro. Biochem. Pharmacol. 2006, 71:1324-1330.
9. Dugina V., Fontao L., Chaponnier C., Vasiliev J., Gabbiani G. Focal adhesion features during myofibroblastic differentiation are controlled by intracellular and extracellular factors. J. Cell Sci. 2001, 114:3285-3296.
10. Fritz G., Just I., Wollenberg P., Aktories K. Differentiation-induced increase in Clostridium botulinum C3 exoenzyme-catalyzed ADP-ribosylation of the small GTP-binding protein Rho. Eur. J. Biochem. 1994, 223:909-916.
11. Fujii T., Onohara N., Maruyama Y., Tanabe S., Kobayashi H., Fukutomi M., Nagamatsu Y., Nishihara N., Inoue R., Sumimoto H., Shibasaki F., Nagao T., Nishida M., Kurose H. Galpha12/13-mediated production of reactive oxygen species is critical for angiotensin receptor-induced NFAT activation in cardiac fibroblasts. J. Biol. Chem. 2005, 280:23041-23047.
12. Gabbiani G. The myofibroblast in wound healing and fibrocontractive diseases. J. Pathol. 2003, 200:500-503.
13. Gauthaman K., Manasi N., Bongso A. Statins inhibit the growth of variant human embryonic stem cells and cancer cells in vitro but not normal human embryonic stem cells. Br. J. Pharmacol. 2009, 157:962-973.
14. Goldstein J.L., Brown M.S. Regulation of the mevalonate pathway. Nature 1990, 343:425-430.
15. Guijarro C., Blanco-Colio L.M., Ortego M., Alonso C., Ortiz A., Plaza J.J., Díaz C., Hernández G., Egido J. 3-Hydroxy-3-methylglutaryl coenzyme a reductase and isoprenylation inhibitors induce apoptosis of vascular smooth muscle cells in culture. Circ. Res. 1998, 83:490-500.
16. Hall A. Rho GTPases and the actin cytoskeleton. Science 1998, 279:509-514.
17. Hayashidani S., Tsutsui H., Shiomi T., Suematsu N., Kinugawa S., Ide T., Wen J., Takeshita A. Fluvastatin, a 3-hydroxy-3-methylglutaryl coenzyme a reductase inhibitor, attenuates left ventricular remodeling and failure after experimental myocardial infarction. Circulation 2002, 105:868-873.
18. He Y.P., Zhao L.Y., Zheng Q.S., Liu S.W., Zhao X.Y., Lu X.L., Niu X.L., Li X. Involvement of ERK and AKT signaling in the growth effect of arginine vasopressin on adult rat cardiac fibroblast and the modulation by simvastatin. Mol. Cell. Biochem. 2008, 317:33-41.
19. Hinz B. Masters and servants of the force: the role of matrix adhesions in myofibroblast force perception and transmission. Eur. J. Cell Biol. 2006, 85:175-181.
20. Joy T.R., Hegele R.A. Narrative review: statin-related myopathy. Ann. Intern. Med. 2009, 150:858-868.
21. Li X., Liu L., Tupper J.C., Bannerman D.D., Winn R.K., Sebti S.M., Hamilton A.D., Harlan J.M. Inhibition of protein geranylgeranylation and RhoA/RhoA kinase pathway induces apoptosis in human endothelial cells. J. Biol. Chem. 2002, 277:15309-15316.
22. Long C.S., Brown R.D. The cardiac fibroblast, another therapeutic target for mending the broken heart?. J. Mol. Cell. Cardiol. 2002, 34:1273-1278.
23. Moulin V., Larochelle S., Langlois C., Thibault I., Lopez-Vallé C.A., Roy M. Normal skin wound and hypertrophic scar myofibroblasts have differential responses to apoptotic inductors. J. Cell. Physiol. 2004, 198:350-358.
24. Moore M., Marroquin B.A., Gugliotta W., Tse R., White S.R. Rho kinase inhibition initiates apoptosis in human airway epithelial cells. Am. J. Respir. Cell Mol. Biol. 2004, 30:379-387.
25. Ogata Y., Takahashi M., Takeuchi K., Ueno S., Mano H., Ookawara S., Kobayashi E., Ikeda U., Shimada K. Fluvastatin induces apoptosis in rat neonatal cardiac myocytes: a possible mechanism of statin-attenuated cardiac hypertrophy. J. Cardiovasc. Pharmacol. 2002, 40:907-915.
26. Olson E.R., Shamhart P.E., Naugle J.E., Meszaros J.G. Angiotensin II-induced extracellular signal-regulated kinase 1/2 activation is mediated by protein kinase C delta and intracellular calcium in adult rat cardiac fibroblasts. Hypertension 2008, 51:704-711.
27. Porter K.E., Turner N.A., O'Regan D.J., Balmforth A.J., Ball S.G. Simvastatin reduces human atrial myofibroblast proliferation independently of cholesterol lowering via inhibition of RhoA. Cardiovasc. Res. 2004, 61:745-755.
28. Roberts P.J., Mitin N., Keller P.J., Chenette E.J., Madigan J.P., Currin R.O., Cox A.D., Wilson O., Kirschmeier P., Der C.J. Rho family GTPase modification and dependence on CAAX motif-signaled posttranslational modification. J. Biol. Chem. 2008, 283:25150-25163.
29. Sacks F.M., Pfeffer M.A., Moye L.A., Rouleau J.L., Rutherford J.D., Cole T.G., Brown L., Warnica J.W., Arnold J.M., Wun C.C., Davis B.R., Braunwald E. The effect of pravastatin on coronary events after myocardial infarction in patients with average cholesterol levels, Cholesterol and Recurrent Events Trial investigators. N. Engl. J. Med. 1996, 335:1001-1009.
30. Stark W.W., Blaskovich M.A., Johnson B.A., Qian Y., Vasudevan A., Pitt B., Hamilton A.D., Sebti S.M., Davies P. Inhibiting geranylgeranylation blocks growth and promotes apoptosis in pulmonary vascular smooth muscle cells. Am. J. Physiol. 1998, 275:L55-L63.
31. Sun Y., Kiani M.F., Postlethwaite A.E., Weber K.T. Infarct scar as living tissue. Basic Res. Cardiol. 2002, 97:343-347.
32. Takemoto M., Liao J.K. Pleiotropic effects of 3-hydroxy-3-methylglutaryl coenzyme a reductase inhibitors. Arterioscler. Thromb. Vasc. Biol. 2001, 21:1712-1719.
33. Tanaka K., Honda M., Takabatake T. Anti-apoptotic effect of atorvastatin, a 3-hydroxy-3-methylglutaryl coenzyme a reductase inhibitor, on cardiac myocytes through protein kinase C activation. Clin. Exp. Pharmacol. Physiol. 2004, 31:360-364.
34. Tsai C.T., Lai L.P., Kuo K.T., Hwang J.J., Hsieh C.S., Hsu K.L., Tseng C.D., Tseng Y.Z., Chiang F.T., Lin J.L. Angiotensin II activates signal transducer and activators of transcription 3 via Rac1 in atrial myocytes and fibroblasts: implication for the therapeutic effect of statin in atrial structural remodeling. Circulation 2008, 117:344-355.
35. van de Donk N.W., Schotte D., Kamphuis M.M., van Marion A.M., van Kessel B., Bloem A.C., Lokhorst H.M. Protein geranylgeranylation is critical for the regulation of survival and proliferation of lymphoma tumor cells. Clin. Cancer Res. 2003, 9:5735-5748.
36. Vivar R., Soto C., Copaja M., Mateluna F., Aranguiz P., Munoz J.P., Chiong M., Garcia L., Letelier A., Thomas W.G., Lavandero S., Díaz-Araya G. Phospholipase C/protein kinase C pathway mediates angiotensin II-dependent apoptosis in neonatal rat cardiac fibroblasts expressing AT1 receptor. J. Cardiovasc. Pharmacol. 2008, 52:184-190.
37. Watts K.L., Sampson E.M., Schultz G.S., Spiteri M.A. Simvastatin inhibits growth factor expression and modulates profibrogenic markers in lung fibroblasts. Am. J. Respir. Cell Mol. Biol. 2005, 32:290-300.
38. Weber K.T., Sun Y., Tyagi S.C., Cleutjens J.P. Collagen network of the myocardium: function, structural remodeling and regulatory mechanisms. J. Mol. Cell. Cardiol. 1994, 26:279-292.