Firm wheat-germ cell-free system with extended vector usage for high-throughput protein screening

Journal of Bioscience and Bioengineering

Volumn 112, Issue 2, 2011, Pages 170-177

  Lee, SungGa ^a   Lassalle, Michael W ^a  

^a EHIME UNIVERSITY   (Japan)

Author keywords

In vitro protein expression; Polymerase chain reaction; Protein screening; Split primer; Wheat germ cell free system

Indexed keywords

CELL-FREE EXTRACTS; CELL-FREE SYSTEM; CHAIN REACTION; CULTURE MEDIUM; HIGH-THROUGHPUT; PROTEIN EXPRESSIONS; ROBOTIC AUTOMATION; SPLIT-PRIMER; THERMO DYNAMIC ANALYSIS; WHEAT GERM; WHEAT-GERM CELL FREE-SYSTEM;

CELL CULTURE; ESCHERICHIA COLI; POLYMERASE CHAIN REACTION; PROTEINS; RNA; ROBOTICS; THERMOANALYSIS; THROUGHPUT;

CELLS;

DNA; PLASMID DNA; PROTEIN C MYB; RNA; RNA POLYMERASE; UBIQUITIN;

ARTICLE; BACTERIAL GROWTH; BACTERIUM CULTURE; CELL FREE SYSTEM; CELL GROWTH; CONTROLLED STUDY; CULTURE MEDIUM; ESCHERICHIA COLI; GENE AMPLIFICATION; GENE VECTOR; GENETIC TRANSCRIPTION; HIGH THROUGHPUT SCREENING; NONHUMAN; PLASMID; POLYMERASE CHAIN REACTION; PROMOTER REGION; PROTEIN ANALYSIS; RNA TRANSLATION; RNA VECTOR; ROBOTICS; THERMODYNAMICS; WESTERN BLOTTING; WHEAT GERM;

CELL-FREE SYSTEM; CULTURE MEDIA; DNA; DNA PRIMERS; ESCHERICHIA COLI; GENETIC VECTORS; HIGH-THROUGHPUT SCREENING ASSAYS; PLASMIDS; POLYMERASE CHAIN REACTION; PROTEIN BIOSYNTHESIS; PROTEINS; PROTEOMICS; TRANSCRIPTION, GENETIC; TRITICUM;

ESCHERICHIA COLI; TRITICUM AESTIVUM;

EID: 79960710576     PISSN: 13891723     EISSN: 13474421     Source Type: Journal    
DOI: 10.1016/j.jbiosc.2011.04.006     Document Type: Article

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