Mutant T7 RNA polymerase is capable of catalyzing DNA primer extension reaction

FEBS Letters

Volumn 423, Issue 2, 1998, Pages 189-192

  Rusakova, E E ^a   Tunitskaya, V L ^a   Memelova, L V ^a   Kochetkova, S V ^a   Kostyuk, D A ^a   Kochetkov, S N ^a  

^a ENGELHARDT INSTITUTE OF MOLECULAR BIOLOGY   (Russian Federation)

Author keywords

Bacteriophage T7 RNA polymerase; Mutant; Primer extension

Indexed keywords

DEOXYRIBONUCLEOSIDE; MUTANT PROTEIN; NUCLEOSIDE TRIPHOSPHATE; PRIMER DNA; RNA POLYMERASE;

ARTICLE; BACTERIOPHAGE T7; CATALYSIS; CONTROLLED STUDY; DNA TRANSCRIPTION; NONHUMAN; PRIORITY JOURNAL;

BACTERIOPHAGES; DEOXYRIBONUCLEOTIDES; DNA PRIMERS; DNA-DIRECTED DNA POLYMERASE; DNA-DIRECTED RNA POLYMERASES; OLIGODEOXYRIBONUCLEOTIDES; VIRAL PROTEINS;

EID: 0032548781     PISSN: 00145793     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0014-5793(98)00058-1     Document Type: Article

References (20)

1. Kornberg A. Methods Enzymol. 182:1990;783-788.
2. Sousa R., Rose J.P., Chung Y.J., Lafer E.M., Wang B.-C. Protein Struct. Funct. Genet. 5:1989;266-270.
3. Kostyuk D.A., Dragan S.M., Lyakhov D.L., Rechinsky V.O., Tunitskaya V.L., Chernov B.K., Kochetkov S.N. FEBS Lett. 369:1995;165-168.
4. Tunitskaya V.L., Memelova L.V., Kostyuk D.A., Gudima S.O., Kochetkov S.N. Mol. Biol. (Moscow). 31:1997;353-358.
5. Moroney S.E., Piccirilli J.A. Biochemistry. 30:1991;10343-10349.
6. Milligan J.F., Groebe D.R., Witherel G.W., Uhlenbeck O.C. Nucleic Acids Res. 15:1987;8783-8789.
7. Daube S.S., von Hippel P.H. Science. 258:1992;1320-1324.
8. Daube S.S., von Hippel P.H. Biochemistry. 33:1994;340-347.
9. Cazenave C., Uhlenbeck O.C. Proc. Natl. Acad. Sci. USA. 91:1994;6972-6976.
10. Maslak V., Martin C.T. Biochemistry. 32:1993;4281-4285.
11. Triana-Alonso F.J., Dabrowski M., Wadzack J., Nierhaus K.Y. J. Biol. Chem. 270:1995;6298-6307.
12. Krupp G. Nucleic Acids Res. 17:1989;3023-3036.
13. Sousa R., Padilla R. EMBO J. 14:1995;4609-4621.
14. Atkinson, T. and Smith, M. (1975) in: Oligonucleotide Synthesis. A Practical Approach (Gait, M.J., Ed.) Vol. 3, pp. 35-81, IRL Press, Oxford-Washington DC.
15. Maniatis, T., Fritsch, E.F. and Sambrook, J. (1982) in: Molecular Cloning: A Laboratory Manual. Cold Spring Harbor University Press, Cold Spring Harbor, NY.
16. Rechinsky V.O., Dragan S.M., Lyakhov D.L., Kochetkov S.N. Mol. Biol. (Moscow). 25:1991;1588-1593.
17. Promega Protocol and Application Guide (2nd edn.) (1991) Promega Corporation, Madison, WI.
18. Gudima S.O., Kazantseva E.G., Kostyuk D.A., Shchaveleva I.L., Grishchenko O.I., Memelova L.V., Kochetkov S.N. Nucleic Acids Res. 25:1997;4614-4618.
19. Gudima, S.O., Kostyuk, D.A., Kazantseva, E.G., Grishchenko, O.I., Memelova, L.V., Tunitskaya, V.L. and Kochetkov, S.N., manuscript in preparation.
20. Sousa R., Patra D., Lafer E.M. J. Mol. Biol. 224:1992;319-334.