A tricistronic human adrenodoxin reductase-adrenodoxin-cytochrome P450 27A1 vector system for substrate hydroxylation in Escherichia coli

Protein Expression and Purification

Volumn 79, Issue 2, 2011, Pages 231-236

  Salamanca Pinzón, S Giovanna ^a   Guengerich, F Peter ^a  

^a VANDERBILT UNIVERSITY   (United States)

Author keywords

Adrenodoxin; Cholesterol; Cytochrome P450; Tricistronic vector; Vitamin D

Indexed keywords

ADRENODOXIN; CHOLESTEROL; COLECALCIFEROL; CYTOCHROME P450; FERREDOXIN NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE REDUCTASE; HYBRID PROTEIN; NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE;

ANIMAL; ARTICLE; CATTLE; CHEMISTRY; ELECTROSPRAY MASS SPECTROMETRY; ESCHERICHIA COLI; GENE VECTOR; GENETICS; HUMAN; HYDROXYLATION; LIQUID CHROMATOGRAPHY; METABOLISM; METHODOLOGY; MITOCHONDRION; PROTEIN BINDING; PROTEIN ENGINEERING; TANDEM MASS SPECTROMETRY; WESTERN BLOTTING;

ADRENODOXIN; ANIMALS; BLOTTING, WESTERN; CATTLE; CHOLECALCIFEROL; CHOLESTEROL; CHROMATOGRAPHY, LIQUID; CYTOCHROME P-450 ENZYME SYSTEM; ESCHERICHIA COLI; FERREDOXIN-NADP REDUCTASE; GENETIC VECTORS; HUMANS; HYDROXYLATION; MITOCHONDRIA; NADP; PROTEIN BINDING; PROTEIN ENGINEERING; RECOMBINANT FUSION PROTEINS; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; TANDEM MASS SPECTROMETRY;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; EUKARYOTA;

EID: 79960674155     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2011.05.008     Document Type: Article

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