Cytochrome b5 coexpression increases the CYP2E1-dependent mutagenicity of dialkylnitrosamines in methyltransferase-deficient strains of Salmonella typhimurium

Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis

Volumn 484, Issue 1-2, 2001, Pages 61-68

  Cooper, Matthew T ^a   Porter, Todd D ^a,b  

^a UNIVERSITY OF KENTUCKY   (United States)

^b University of Kentucky   (United States)

Author keywords

Human CYP2E1; Human cytochrome b5; Mutagenicity; N nitrosamine; Salmonella typhimurium YG7104

Indexed keywords

COMPLEMENTARY DNA; CYTOCHROME B5; CYTOCHROME P450 REDUCTASE; DIBUTYLNITROSAMINE; DIETHYLNITROSAMINE; DIMETHYLNITROSAMINE; DIPROPYLNITROSAMINE; METHYLTRANSFERASE; NITROSAMINE;

ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME SPECIFICITY; FOOD CONTAMINATION; MUTAGEN TESTING; MUTAGENICITY; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION;

ANIMALS; BACTERIAL PROTEINS; BIOTRANSFORMATION; CYTOCHROME P-450 CYP2E1; CYTOCHROMES B5; ESCHERICHIA COLI PROTEINS; GENE EXPRESSION; HUMANS; METHYLTRANSFERASES; MUTAGENICITY TESTS; MUTAGENS; NADPH-FERRIHEMOPROTEIN REDUCTASE; NITROSAMINES; O(6)-METHYLGUANINE-DNA METHYLTRANSFERASE; PLASMIDS; RATS; RECOMBINANT PROTEINS; SALMONELLA TYPHIMURIUM; TRANSCRIPTION FACTORS;

ADA; SALMONELLA TYPHIMURIUM; TYPHIMURIUM;

EID: 0035852112     PISSN: 00275107     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0027-5107(01)00236-6     Document Type: Article

References (30)

1. 5, its functions, structure and membrane topology
2. 5
3. 5
4. Baculovirus expression and purification of human and rat cytochrome P450 2E1
5. Expression and coupling of human cytochrome P450 2E1 and NADPH-cytochrome P450 oxidoreductase in dual expression and coinfection systems with baculovirus in insect cells
6. 5 on the stoichiometry of P450 2E1-catalyzed reactions
7. Mutagenicity of nitrosamines in methyltransferase-deficient strains of Salmonella typhimurium coexpressing human cytochrome P450 2E1 and reductase
8. 6-methylguanine
9. Metabolic activation of N-alkylnitrosamines in genetically engineered Salmonella typhimurium expressing CYP2E1 or CYP2A6 together with human NADPH-cytochrome P450 reductase
10. Strategies to enhance the coexpression of cytochrome P450 2E1 and reductase in bacteria
11. 5 mRNA
12. Secretion cloning vectors in Escherichia coli
13. Coexpression of mammalian cytochrome P450 and reductase in Escherichia coli
14. Purified liver microsomal NADPH-cytochrome P-450 reductase. Spectral characterization of oxidation-reduction states
15. Bacterial mutation assays using reverse mutation
16. Effect of the length of alkyl chain on the cytochrome P450 dependent metabolism of N-diakylnitrosamines
17. Recent advances in the construction of bacterial genotoxicity assays
18. Bioactivation of aromatic amines by recombinant human cytochrome P4501A2 expressed in Ames tester strain bacteria: A substitute for activation by mammalian tissue preparations
19. Development of a new genotoxicity test system with Salmonella typhimurium OY1001/1A2 expressing human CYP1A2 and NADPH-P450 reductase
20. Metabolic activation of aromatic amine mutagens by simultaneous expression of human cytochrome P450 1A2, NADPH-cytochrome P450 reductase, and N-acetyltransferase in Escherichia coli
21. Genetic polymorphism of CYP2A6 in relation to cancer
22. Establishment of a Salmonella tester strain highly sensitive to mutagenic heterocylic amines
23. Expression of human cytochrome P450 1A2 in Escherichia coli: A system for biotransformation and genotoxicity studies of chemical carcinogens
24. Escherichia coli MTC, a NADPH cytochrome P450 reductase competent mutagenicity tester strain for the expression of human cytochrome P450: Comparison of three types of expression systems
25. Escherichia coli MTC, a human NADPH P450 reductase competent mutagenicity tester strain for the expression of human cytochrome P450 isoforms 1A1, 1A2, 2A6, 3A4, or 3A5: Catalytic activities and mutagenicity studies
26. 5 on CYP3A4 activity and stability in vivo
27. Participation of rat liver cytochrome P450 2E1 in the activation of N-nitrosodimethylamine and N-nitrosodiethylamine to products genotoxic in an acetyltransferase-overexpressing Salmonella typhimurium strain (NM2009)
28. 5 potentiation of cytochrome P-450 catalytic activity demonstrated by a Vaccinia virus-mediated in situ reconstitution system
29. 5 augments the 1720-lyase activity of human P450c17 without direct electron transfer
30. 5 in yeast