An optimized method for suicide vector-based allelic exchange in Klebsiella pneumoniae

Journal of Microbiological Methods

Volumn 86, Issue 3, 2011, Pages 313-319

  van Aartsen, Jon Jurriaan ^a   Rajakumar Kumar, K ^a,b  

^a UNIVERSITY OF LEICESTER   (United Kingdom)

^b UNIVERSITY HOSPITALS OF LEICESTER NHS TRUST   (United Kingdom)

Author keywords

Allelic exchange; Homologous recombination; Klebsiella pneumoniae; SacB counterselection; Targeted mutagenesis

Indexed keywords

AMPICILLIN; CHLORAMPHENICOL; GENTAMICIN; KANAMYCIN; RECOMBINASE;

ALLELE; ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL STRAIN; BACTERIUM ISOLATE; CONTROLLED STUDY; COUNTERSELECTION; GENE CASSETTE; GENE IDENTIFICATION; GENE LOCUS; GENE MUTATION; GENE VECTOR; GENETIC MANIPULATION; GENETIC SELECTION; HIGH TEMPERATURE; KLEBSIELLA PNEUMONIAE; MOLECULAR CLONING; MULTIDRUG RESISTANCE; NONHUMAN; NUCLEOTIDE SEQUENCE; PHENOTYPE; PLASMID; PRIORITY JOURNAL; SUICIDE VECTOR;

BASE SEQUENCE; DNA NUCLEOTIDYLTRANSFERASES; DNA, BACTERIAL; GENE TRANSFER TECHNIQUES; GENES, TRANSGENIC, SUICIDE; GENETIC VECTORS; GENOME; KLEBSIELLA INFECTIONS; KLEBSIELLA PNEUMONIAE; PLASMIDS;

BACTERIA (MICROORGANISMS); KLEBSIELLA PNEUMONIAE;

EID: 79960644739     PISSN: 01677012     EISSN: 18728359     Source Type: Journal    
DOI: 10.1016/j.mimet.2011.06.006     Document Type: Article

References (24)

1. Ausubel F.M., Brent R., Kingston R.E., Moore D., Seidman J.G., Struhl K. Current Protocols in Molecular Biology 2004, John Wiley & Sons.
2. Blomfield I.C., Vaughn V., Rest R.F., Eisenstein B.I. Allelic exchange in Escherichia coli using the Bacillus subtilis sacB gene and a temperature-sensitive pSC101 replicon. Mol. Microbiol. 1991, 5(6):1447-1457.
3. Choi K.H., Schweizer H.P. An improved method for rapid generation of unmarked Pseudomonas aeruginosa deletion mutants. BMC Microbiol. 2005, 5:1-11.
4. Choi K., Gaynor J., White K., Lopez C., Bosio C., Karkhoff-Schweizer R.R., Schweizer H.P. A Tn7-based broad-range bacterial cloning and expression system. Nat. Methods 2005, 2(6):443-448.
5. Chuang Y.P., Fang C.T., Lai S.Y., Chang S.C., Wang J.T. Genetic determinants of capsular serotype K1 of Klebsiella pneumoniae causing primary pyogenic liver abscess. J. Infect. Dis. 2006, 193(5):645-654.
6. Clements A., Tull D., Jenney A.W., Farn J.L., Kim S.-H., Bishop R.E., McPhee J.B., Hancock R.E.W., Hartland E.L., Pearse M.J., Wijburg O.L.C., Jackson D.C., McConville M.J., Strugnell R.A. Secondary acylation of Klebsiella pneumoniae lipopolysaccharide contributes to sensitivity to antibacterial peptides. J. Biol. Chem. 2007, 282(21):15569-15577.
7. Datsenko K., Wanner B.L. One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. PNAS 2000, 97(12):6640-6645.
8. Fournet-Fayard S., Joly B., Forestier C. Transformation of wild type Klebsiella pneumoniae with plasmid DNA by electroporation. J. Microbiol. Methods 1995, 24(1):49-54.
9. Fouts D.E., Tyler H.L., DeBoy R.T., Daugherty S., Ren Q., Badger J.H., Durkin A.S., Huot H., Shrivastava S., Kothari S., Dodson R.J., Mohamoud Y., Khouri H., Roesch L.F., Krogfelt K.A., Struve C., Triplett E.W., Methe B.A. Complete genome sequence of the N2-fixing broad host range endophyte Klebsiella pneumoniae 342 and virulence predictions verified in mice. PLoS Genet. 2008, 4(7):1-18.
10. Gay P., Le Coq D., Steinmetz M., Berkelman T., Kado C.I. Positive selection procedure for entrapment of insertion sequence elements in gram-negative bacteria. J. Bacteriol. 1985, 164(2):918-921.
11. Hoang T.T., Karkhoff-Schweizer R.R., Kutchma A.J., Schweizer H.P. A broad-host-range Flp-FRT recombination system for site-specific excision of chromosomally-located DNA sequences: application for isolation of unmarked Pseudomonas aeruginosa mutants. Gene 1998, 212(1):77-86.
12. Li M., Elledge S.J. Harnessing homologous recombination in vitro to generate recombinant DNA via SLIC. Nat. Methods 2007, 4(3):251-256.
13. Philippe N., Alcaraz J.P., Coursange E., Geiselmann J., Schneider D. Improvement of pCVD442, a suicide plasmid for gene allele exchange in bacteria. Plasmid 2004, 51(3):246-255.
14. Podschun R., Ullmann U. Klebsiella spp. as nosocomial pathogens: epidemiology, taxonomy, typing methods, and pathogenicity factors. Clin. Microbiol. Rev. 1998, 11(4):589-603.
15. Poteete A., Rosadini C., St Pierre C. Gentamicin and other cassettes for chromosomal gene replacement in Escherichia coli. Biotechniques 2006, 41(3):261-264.
16. Reyrat J., Pelicic V., Gicquel B.R. Counterselectable markers: untapped tools for bacterial genetics and pathogenesis. Infect. Immun. 1998, 66(9):4011-4017.
17. Rice L.B. Progress and challenges in implementing the research on ESKAPE pathogens. Infect. Control Hosp. Epidemiol. 2010, 31(Suppl. 1):S7-S10.
18. Rosen D.A., Pinkner J.S., Jones J.M., Walker J.N., Clegg S., Hultgren S.J. Utilization of an intracellular bacterial community pathway in Klebsiella pneumoniae urinary tract infection and the effects of FimK on type 1 pilus expression. Infect. Immun. 2008, 76(7):3337-3345.
19. Simon R., Priefer U., Pühler A. A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in gram negative bacteria. Bio/Technology 1983, 1(9):784-791.
20. Struve C., Bojer M., Krogfelt K.A. Characterization of Klebsiella pneumoniae type 1 fimbriae by detection of phase variation during colonization and infection and impact on virulence. Infect. Immun. 2008, 76(9):4055-4065.
21. Struve C., Bojer M., Krogfelt K.A. Identification of a conserved chromosomal region encoding Klebsiella pneumoniae type 1 and type 3 fimbriae and assessment of the role of fimbriae in pathogenicity. Infect. Immun. 2009, 77(11):5016-5024.
22. Tsai F.-C. Pyogenic liver abscess as endemic disease, Taiwan. Emerg. Infect. Dis. 2008, 14(10):1592-1600.
23. Viret J.F. Meganuclease I-SceI as a tool for the easy subcloning of large DNA fragments devoid of selection marker. Biotechniques 1993, 14(3):325-326.
24. Zhang G., Ma B., Xu X., Li C., Wang L. Fast conversion of glycerol to 1,3-propanediol by a new strain of Klebsiella pneumoniae. Biochem. Eng. J. 2007, 37(3):256-260.