Gentamicin and other cassettes for chromosomal gene replacement in Escherichia coli

BioTechniques

Volumn 41, Issue 3, 2006, Pages 261-264

  Poteete, Anthony R ^a   Rosadini, Charles ^a   St Pierre, Christine ^a  

^a UNIVERSITY OF MASSACHUSETTS MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMPICILLIN; CHLORAMPHENICOL; GENTAMICIN; KANAMYCIN; SPECTINOMYCIN; TETRACYCLINE; ANTIINFECTIVE AGENT; PRIMER DNA;

AADA1 GENE; ANTIBIOTIC RESISTANCE; BACTERIAL CHROMOSOME; BACTERIAL GENE; BACTERIUM MUTANT; ESCHERICHIA COLI; GENE CASSETTE; GENETIC RECOMBINATION; NONHUMAN; NUCLEOTIDE SEQUENCE; OPERON; REVIEW; ARTICLE; CHEMISTRY; GENETIC PROCEDURES; GENETICS; MOLECULAR GENETICS; PLASMID;

ESCHERICHIA COLI;

ANTI-BACTERIAL AGENTS; BASE SEQUENCE; CHROMOSOMES, BACTERIAL; DNA PRIMERS; DRUG RESISTANCE, MICROBIAL; ESCHERICHIA COLI; GENES, BACTERIAL; GENETIC TECHNIQUES; GENTAMICINS; MOLECULAR SEQUENCE DATA; PLASMIDS; RECOMBINATION, GENETIC;

EID: 34047235530     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/000112242     Document Type: Review

References (14)

1. Murphy, K.C. 1998. Use of bacteriophage λ recombination functions to promote gene replacement in Escherichia coli. J. Bacteriol. 180:2063-2071.
2. Zhang, Y., F. Buchholz, J.P.P. Muyrers, and A.F. Stewart. 1998. A new logic for DNA engineering using recombination in Escherichia coli. Nat. Genet. 20:123-128.
3. Yu, D., H.M. Ellis, E.-C. Lee, N.A. Jenkins, N.G. Copeland, and D.L. Court. 2000. An efficient recombination system for chromosome engineering in Escherichia coli. Proc. Natl. Acad. Sci. USA 97:5978-5983.
4. Datsenko, K.A. and B.L. Wanner. 2000. One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc. Natl. Acad. Sci. USA 97:6640-6645.
5. Poteete, A.R. 2004. Modulation of DNA repair and recombination by the bacteriophage lambda Orf function in Escherichia coli K-12. J. Bacteriol. 186:2699-2707.
6. Prentki, P. and H.M. Krisch. 1982. A modified pBR322 vector with improved properties for the cloning, recovery, and sequencing of blunt-ended DNA fragments. Gene 17:189-196.
7. Churchward, G., D. Belin, and Y. Nagamine. 1984. A pSC101-derived plasmid which shows no sequence homology to other commonly used cloning vectors. Gene 31:165-17l.
8. Hirsch, P.R., C.L. Wang, and M.J. Woodward. 1986. Construction of a Tn5 derivative determining resistance to gentamicin and spectinomycin using a fragment cloned from R1033. Gene 48:203-209.
9. Schweizer, H.D. 1993. Small broad-host-range gentamycin resistance gene cassettes for site-specific insertion and deletion mutagenesis. BioTechniques 15:831-833.
10. Gold, L. and J.D. Stormo. 1990. High-level translation initiation. Methods Enzymol. 185:89-94.
11. Jensen, P.R. and K. Hammer. 1998. The sequence of spacers between the consensus sequences modulates the strength of prokaryotic promoters. Appl. Environ. Microbiol. 64:82-87.
12. Murphy, K.C. and K.G. Campellone. 2003. Lambda Red-mediated recombinogenic engineering of enterohemorrhagic and enteropathogenic E. coli. BMC Mol. Biol. 4:11.
13. Wohlleben, W., W. Arnold, L. Bissonnette, A. Pelletier, A. Tanguay, P.H. Roy, G.C. Gamboa, G.F. Barry, et al. 1989. On the evolution of Tn21-like multiresistance transposons: sequence analysis of the gene (aacC1) for gentamicin acetyltransferase-3-I(AAC(3)-I), another member of the Tn21-based expression cassette. Mol. Gen. Genet. 217:202-208.
14. Coulondre, C., J.H. Miller, PJ. Farabaugh, and W. Gilbert. 1978. Molecular basis of base substitution hotspots in Escherichia coli. Nature 274:775-780.