Discrimination of recombinant from natural human growth hormone using DNA aptamers

Journal of Biomolecular Techniques

Volumn 22, Issue 1, 2011, Pages 27-36

  Bruno, John G ^a   Carrillo, Maria P ^a   Phillips, Taylor ^a   Edge, Allison ^a  

^a Operational Technologies Corporation   (United States)

Author keywords

Doping; ELISA; Post translational modification; SELEX; Somatotropin

Indexed keywords

APTAMER; BOVINE SERUM ALBUMIN; COLLAGEN; DIMER; DNA; EPITOPE; HUMAN GROWTH HORMONE; MYOGLOBIN; RECOMBINANT GROWTH HORMONE; VIRUS ENVELOPE PROTEIN; RECOMBINANT PROTEIN;

ADSORPTION; AMINO ACID SEQUENCE; ARTICLE; ATHLETE; CONTROLLED STUDY; CROSS REACTION; DNA BINDING; DNA SEQUENCE; ENZYME LINKED IMMUNOSORBENT ASSAY; HORMONE DETERMINATION; LABORATORY TEST; MICROPARTICLE ENZYME IMMUNOASSAY; MOLECULAR SIZE; NUCLEOTIDE SEQUENCE; POLYACRYLAMIDE GEL ELECTROPHORESIS; SCREENING; BIOASSAY; COMPARATIVE STUDY; CONFORMATION; DOPING; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METHODOLOGY; MOLECULAR GENETICS;

AMINO ACID SEQUENCE; APTAMERS, NUCLEOTIDE; ATHLETES; BASE SEQUENCE; BIOLOGICAL ASSAY; DOPING IN SPORTS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; EPITOPES; HUMAN GROWTH HORMONE; HUMANS; MOLECULAR SEQUENCE DATA; NUCLEIC ACID CONFORMATION; RECOMBINANT PROTEINS;

EID: 79960610786     PISSN: 15240215     EISSN: 19434731     Source Type: Journal    
DOI: None     Document Type: Article

References (23)

1. Bidlingmaier M, Strasburger CJ. Technology insight: detecting growth hormone abuse in athletes. Nat Clin Pract Endocrinol Metab 2007;3:769-777.
2. Hepner F, Cszasar E, Roitinger E, Lubec G. Mass spectrometrical analysis of recombinant human growth hormone (Genotropin) reveals amino acid substitution in 2% of the expressed protein. Proteome Sci 2005;3:1-12.
3. Hepner F, Csaszar E, Roitinger E, Pollak A, Lubec G. Mass spectrometrical analysis of recombinant human growth hormone Norditropin reveals amino acid exchange at M14-V14 rhGH. Proteomics 2006;6:775-784.
4. Ikehara M, Ohtsuka E, Tokunaga T, et al. Synthesis of a gene for human growth hormone and its expression in Escherichia coli. Proc Natl Acad Sci USA 1984;81:5956-5960.
5. McHugh CM, Park RT, Sönksen PH, Holt RI. Challenges in detecting the abuse of growth hormone in sport. Clin Chem 2005;51:1587-1593.
6. Chung L, Clifford D, Buckley M, Baxter RC. Novel biomarkers of human growth hormone action from serum proteomic profiling using protein chip mass spectrometry. J Endocrinol Metab 2006;91:671-677.
7. Bruno JG, Carrillo MP, Phillips T, King B. Development of DNA aptamers for cytochemical detection of acetylcholine. In Vitro Cell Devel Biol Anim 2008;44:63-72.
8. Bruno JG, Carrillo MP, Cadieux CL, Lenz DL, Cerasoli DM, Phillips T. DNA aptamers developed against a soman derivative cross-react with methylphosphonic acid but not with the flanking hydrophobic groups. J Mol Recognit 2009;22:197-204.
9. Jenison RD, Gill SC, Pardi A, Polisky B. High-resolution molecular discrimination by RNA. Science 1994;263:1425-1429.
10. Jayasena SD. Aptamers: an emerging class of molecules that rival antibodies in diagnostics. Clin Chem 1999;45:1628-1650.
11. Vivekananda J, Kiel JL. Anti-Francisella tularensis DNA aptamers detect tularemia antigen from different subspecies by aptamer-linked immobilized sorbent assay. Lab Invest 2006;86: 610-618.
12. Calik P, Balci O, Ozdamar TH. Human growth hormonespecific aptamer identification using improved oligonucleotide ligand evolution method. Prot Expr Purif 2010;69:21-28.
13. Bruno JG, Carrillo MP, Crowell R. Preliminary development of DNA aptamer-Fc conjugate opsonins. J Biomed Mater Res A 2009;90:1152-1161.
14. Bruno JG, Carrillo MP, Phillips T, Andrews CJ. A novel screening method for competitive FRET-aptamers applied to E. coli assay development. J Fluoresc 2010;20:1211-1223.
15. Bruno JG, Carrillo MP, Phillips T. Development of DNA aptamers to a foot-and-mouth disease peptide for competitive FRET-based detection. J Biomol Tech 2008;19:109-115.
16. Bruno JG, Carrillo MP, Phillips T. Effects of immobilization chemistry on enzyme-linked aptamer assays for Leishmania surface antigens. J Clin Ligand Assay 2007;30:37-43.
17. Bruno JG, Kiel JL. Use of magnetic beads in selection and detection of biotoxin aptamers by ECL and enzymatic methods. BioTechniques 2002;32:178-183.
18. Gruber AR, Lorenz R, Bernhart SH, Neuböck R, Hofacker IL. The Vienna RNA website. Nucleic Acids Res 2008;36:W70-W74.
19. Hofacker IL. Vienna RNA secondary structure server. Nucleic Acids Res 2003;31:3429-3431.
20. SantaLucia J. A unified view of polymer, dumbbell, and oligonucleotide DNA nearest-neighbor thermodynamics. Proc Natl Acad Sci USA 1998;95:1460-1465.
21. Haro LS, Lewis UJ, Garcia M, Bustamante J, Martinez AO, Ling NC. Glycosylated human growth hormone (hGH): a novel 24 kDa hGH-N variant. Biochem Biophys Res Comm 1996;228: 549-556.
22. Cunningham BC, Mulkerrin MG, Wells JA. Dimerization of human growth hormone by zinc. Science 1991;253:545-548.
23. Grigorian AI, Bustamante JJ, Hernandez P, Martinez AO, Haro LS. Extraordinarily stable disulfide-linked homodimer of human growth hormone. Protein Sci 2004;14:902-913.