Extraordinarily stable disulfide-linked homodimer of human growth hormone

Protein Science

Volumn 14, Issue 4, 2005, Pages 902-913

  Grigorian, Alexei L ^a   Bustamante, Juan J ^b   Hernandez, Peter ^c   Martinez, Andrew O ^d   Haro, Luis S ^d  

^a UNIVERSITY OF ALABAMA SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF KANSAS MEDICAL CENTER   (United States)

^c Palo Alto College   (United States)

^d UNIVERSITY OF TEXAS AT SAN ANTONIO   (United States)

Author keywords

Dimer; Human growth hormone; Isoform; Mass spectrometry; Preparative electrophoresis; Protein structure; Purification

Indexed keywords

AMINO ACID; DIMER; DISULFIDE; EDETIC ACID; EGTAZIC ACID; GENE PRODUCT; HUMAN GROWTH HORMONE; MERCAPTOETHANOL; METAL ION; MONOMER; POLYPEPTIDE;

ALKYLATION; AMINO ACID SEQUENCE; ARTICLE; CHROMATOGRAPHY; CONTROLLED STUDY; DENSITOMETRY; HORMONE STRUCTURE; HUMAN; HUMAN TISSUE; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN STRUCTURE; PURIFICATION; WESTERN BLOTTING;

CHELATING AGENTS; DIMERIZATION; DISULFIDES; ELECTROPHORESIS; HUMAN GROWTH HORMONE; HUMANS; MERCAPTOETHANOL; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; PROTEIN SUBUNITS; REDUCING AGENTS; SEQUENCE ANALYSIS, PROTEIN; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

EID: 15244362275     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1110/ps.041048805     Document Type: Article

References (62)

1. Altszuler, N. 1974. Actions of growth hormone on carbohydrate metabolism. In Handbook of physiology, Section 7: Endocrinology, Vol. 4, Part 2 (eds. E. Knobil and W.H. Sawyer), pp. 233-252. American Physiological Society, Washington, DC.
2. Ansorge, W. 1985. Fast and sensitive detection of protein and DNA bands by treatment with potassium permanganate. J. Biochem. Biophys. Methods 11: 13-20.
3. Barrera-Saldana, H.A. 1998. Growth hormone and placental lactogen: Biology, medicine and biotechnology. Gene 211: 11-18.
4. Bauman, D.E. 1999. Bovine somatotropin and lactation: From basic science to commercial application. Domest. Anim. Endocrinol. 17: 101-116.
5. Bauman, D.E., Eisemann, J.H., and Currie, W.B. 1982. Hormonal effects on partitioning of nutrients for tissue growth: Role of growth hormone and prolactin. Fed. Proc. 41: 2538-2544.
6. Baumann, G. 1991. Growth hormone heterogeneity: Genes, isohormones, variants, and binding proteins. Endocrin. Rev. 12: 424-449.
7. -. 1999. Growth hormone heterogeneity in human pituitary and plasma. Horm. Res. 51 (Suppl. 1): 2-6.
8. Boguszewski, C.L. 2003. Molecular heterogeneity of human GH: From basic research to clinical implications. J. Endocrinol. Invest. 26: 274-288.
9. Brostedt, P. and Roos, P. 1988. Isolation of four isomers of the 20,000 dalton variant of human pituitary growth hormone. Prep. Biochem. 18: 277-291.
10. -. 1989. Isolation of dimeric forms of human pituitary growth hormone. Prep. Biochem. 19: 217-229.
11. Brumby, P.J. 1959. The influence of growth hormone on growth in young cattle. N.Z.J. Agric. Rex. 2: 683-689.
12. Chapman, G.E., Rogers, K.M., Brittain, T., Bradshaw, R.A., Bates, O.J., Turner, C., Gary, P.D., and Crane-Robinson, C. 1981. The 20,000 molecular weight variant of human growth hormone. Preparation and some physical and chemical properties. J. Biol. Chem. 256: 2395-2401.
13. Charrier, J. and Martal, J. 1988. Growth hormones. I. Polymorphism. (Minireview). Reprod. Nutr. Dev. 28: 857-887.
14. Cheek, D.B. and Hill, D.E. 1974. Effect of growth hormone on cell and somatic growth. In Handbook of physiology, Section 7, Vol. 4 (eds. R.O. Greep and E.B. Astwood), pp. 159-185. American Physiological Society, Washington, DC.
15. Chen, E.Y., Liao, Y.-C., Smith, D.H., Barrera-Saldana, H.A., Gelinas, R.E., and Seeburg, P.H. 1989. The human growth hormone gene locus: Nucleotide sequence, biology, and evolution. Genomics 4: 479-497.
16. Chene, N., Martal, J., de la Llosa, P., and Charrier, J. 1989. Growth hormones. II. Structure-function relationships. Reprod. Nutr. Dev. 29: 1-25.
17. Chung, C.S., Etherton, T.D., and Wiggins, J.P. 1985. Stimulation of swine growth by porcine growth hormone. J. Animal. Sci. 60: 118-130.
18. Clauser, K.R., Baker, P.R., and Burlingame, A.L. 1999. Role of accurate mass measurement (+/-10 ppm) in protein identification strategies employing MS or MS/MS and database searching. Anal. Chem. 71: 2871-2882.
19. Creighton, T.E. 1980. Counting integral numbers of amino acid residues per polypeptide chain. Nature 284: 487-488.
20. Cunningham, B.C., Mulkerrin, M.G., and Wells, J.A. 1991. Dimerization of human growth hormone by zinc. Science 253: 545-548.
21. Diaz, M.J., Dominguez, F., Haro, L.S., Ling, N., and Devesa, J. 1993. A 12-kilodalton N-glycosylated growth hormone-related peptide is present in human pituitary extracts. J. Clin. Endocrin. Metab. 77: 134-138.
22. Evans, H.M. and Simpson, M.E. 1931. Hormones of the anterior hypophysis. Am. J. Physiol. 98: 511-546.
23. Goodman, H.M. and Schwartz, J. 1974. Growth hormone and lipid metabolism. In Handbook of physiology, Section 7: Endocrinology. Vol. 4, Part 2 (eds. E. Knobil and W.H. Sawyer), pp. 211-231. American Physiological Society, Washington, DC.
24. Graf, L., Li, C.H., and Bewley, T.A. 1975. Selective reduction and alkylation of the COOH-terminal disulfide bridge in bovine growth hormone. Int. J. Peptide Protein Res. 7: 467-473.
25. Grigorian, A.L., Bustamante, J.J., Aguilar. R.M., Martinez, A.O., and Haro, L.S. 2002. 45 kDa Mercaptoethanol-resistant human growth hormone binds to somatogenic and lactogenic receptors. Program of the 84th Annual Meeting of the Endocrine Society, p. 555. San Francisco, CA.
26. Grigorian, A.L., Bustamante, J.J., Aguilar, R.M., Muñoz, J., Martinez, A.O., and Haro, L.S. 2003. Characterization of 45 kDa mercaptoethanol-resistant human growth hormone by radioimmunoassay and bioassays. Program of the 85th Annual Meeting of the Endocrine Society, p. 381. Philadelphia, PA.
27. Hames, B.D. 1998. Gel electrophoresis of proteins: A practical approach, 3rd ed. pp. 34-35. Oxford University Press, Oxford, UK.
28. Haro, L.S., Lewis, U.J., Garcia, M., Bustamante, J., Martinez, A.O., and Ling, N.C. 1996. Glycosylated human growth hormone (hGH): A novel 24 kDa hGH-N variant. Biochem. Biophys. Res. Commun. 228: 549-556.
29. Haro, L.S., Cubriel, A., Bustamante, J., Flores, R., and Martinez, A.O. 1998. Divalent metal cation chelators enhance Chromatographic separation of structurally similar macromolecules: Separation of human growth hormone isoforms. J. Chromat. B 720: 39-47.
30. Hearn, M.T.W., Grego, B., and Chapman, G.E. 1983. Separation and assignment of the tryptic peptides of human growth hormone (hGH) and the 20K Dalton variant by reversed phase high performance liquid chromatography. J. Liquid Chrom. 6: 215-228.
31. Hernandez, P., Bustamante, J., and Haro, L.S. 1998. Analysis of a novel growth hormone variant from human pituitaries. 1998 Annual Meeting of the American Society for Biochemistry and Molecular Biology, Washington, DC. FASEB J. 12: A1363.
32. Ho, K.K., O'Sullivan, A.J., and Hoffman, D.M. 1996. Metabolic actions of growth hormone in man. Endocr. J. 43: S57-S63.
33. Hunkapiller, M.W., Hewick, R.M., Dreyer, W.J., and Hood, L.E. 1983. Isolation of microgram quantities of proteins from polyacrylamide gels for amino acid sequence analysis. Methods Enzymol. 91: 399-413.
34. Jacobs, L.S. and Lee, Y.-C. 1975. Polymeric growth hormone and prolactin in secretory granules: Sulfhydryl bonding masks immunoreactive sites. Program of the 57th Annual Meeting of the Endocrine Society, p. 83. New York.
35. Kostyo, J.J. and Nutting, D.F. 1974. Growth hormone and protein metabolism. In Handbook of physiology. Section 7: Endocrinology, Vol. 4, Part 2 (eds. E. Knobil and W.H. Sawyer), pp. 187-210. American Physiological Society, Washington, DC.
36. Laemmli, U.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.
37. Lewis, U.J. 1992. Growth hormone: What is it and what does it do? Trends Endocrinol. Metab. 3: 117-121.
38. Lewis, U.J., Peterson, S.M., Bonewald, L.F., Seavey, B.K., and VanderLaan, W.P. 1977. An interchain disulfide dimer of human growth hormone. J. Biol. Chem. 252: 3697-3702.
39. Lewis, U.J., Singh, R.N., Tutwiler, G.F., Sigel, M.B., VanderLaan, E.F., and VanderLaan, W.P. 1980. Human growth hormone: A complex of proteins. Recent Prog. Horm. Res. 36: 477-508.
40. Lewis, U.J., Singh, R.N.P., Lewis, L.J., and Abadi, N. 1987. Detection of multiple forms of human growth hormone. In Acromegaly: A century of scientific and clinical progress (eds. R.J. Robbins and S. Melmed), pp. 27-34. Plenum, New York.
41. Lewis, U.J., Sinha, Y.N., and Haro, L.S. 1994. Variant forms and fragmems of human growth hormone in serum. Acta. Paediatr. Suppl. 399: 29-31.
42. Lewis, U.J., Sinha, Y.N., and Lewis, G.P. 2000. Structure and properties of members of the hGH family: A review. Endocrin. J. 47: S1-S8.
43. Lorenson, M.Y. and Jacobs, L.S. 1982. Thiol regulation of protein, growth hormone, and prolactin release from isolated adenohypophysial secretory granules. Endocrinology 110: 1164-1172.
44. Lorenson, M.Y., Robson, D.L., and Jacobs, L.S. 1983. Detectabilily of pituitary PRL and GH by immunoassay is increased by thiols and suppressed by divalent cations. Endocrinology 112: 1880-1882.
45. Machlin, L.J. 1972. The effect of porcine growth hormone on growth and carcass composition of the pig. J. Anim. Sci. 35: 794-800.
46. Mao, B. 1990. Molecular topology and dimerization of recombinant bovine somatotropin. Biopolymers 30: 645-647.
47. Muir, L.A., Wien, S., Dugvette, P.F., Rickes, E.L., and Cordes, E.H. 1983. Effects of endogenous growth hormone and diethylstilbesterol on growth and carcass composition of growing lambs. J. Anim. Sci. 56: 1315-1323.
48. Muñoz, J., Esquivel, J., Bustamante, J.J., Martinez, A.O., and Haro, L.S. 2004. Anti-proliferative effects of a 2-mercaptoethanol resistant 45 kDa human growth hormone variant in MCF-7, MDA-MB-231 and T47D human breast cancer cell lines. Program of the 86th Annual Meeting of the Endocrine Society, p. 375. New Orleans, LA.
49. Nindl, B.C., Kraemer, W.J., Marx, J.O., Tuckow, A.P., and Hymer, W.C. 2003. Growth hormone molecular heterogeneity and exercise. Exerc. Sport Sci. Rev. 31: 161-166.
50. Ochs, D. 1983. Protein contaminants of sodium dodecyl sulfate-polyacrylamide gels. Anal. Biochem. 135: 470-474.
51. Okada, S. and Kopchick, J.J. 2001. Biological effects of growth hormone and its antagonist. Trends Mol. Med. 7: 126-132.
52. Paul-Pletzer, K. and Parness, J. 2001. Elimination of keratin contaminant from 2-mercaptoethanol. Anal. Biochem. 289: 98-99.
53. Renaville, R., Hammadi, M., and Portetelle, D. 2002. Role of the somatotropic axis in the mammalian metabolism. Domest. Anim. Endocrinol. 23: 351-360.
54. Shapiro, S.Z. 1987. Elimination of the detection of an artifactual 65 kDa keratin band from immunoblots. J. Immunol. Methods 102: 143-146.
55. Shevchenko, A., Wilm, M., Vorm, O., and Mann, M. 1996. Mass spectrometric sequencing of proteins from silver-stained polyacrylamide gels. Anal. Chem. 68: 850-858.
56. Singh, R.N.P., Seavey, B.K., and Lewis, U.J. 1974. Heterogeneity of human growth hormone. Endocrine Res. Commun. 1: 449-464.
57. Strobl, J.S. and Thomas, M.J. 1994. Human growth hormone. Pharmac. Rev. 46: 1-34.
58. Talamames, F., Lopez, J., Lewis, U.J., and Wilson, C.B. 1981. Multiple forms of growth hormone: Detection in medium from cultured pituitary adenoma explants. Acta Endocrinol. 98: 8-13.
59. Thamann, T.J. 1998. Raman spectroscopic studies of a dimeric form of recombinant bovine growth hormone. Anal. Biochem. 265: 202-207.
60. Tou, S.S., Violand, B.N., Schlittler, M.R., and Jennings, M.G. 1993. Structural characterization of the two refold dimers of recombinant bovine somatotropin (bST). J. Protein Chem. 12: 237-245.
61. Violand, B.N., Takano, M., Curran, D.F., and Bentle, L.A. 1989. A novel concatenated dimer of recombinant bovine somatotropin. J. Protein Chem. 8: 619-628.
62. Waters, M.J. and Kaye, P.L. 2002. The role of growth hormone in fetal development. Growth Horm. IGF Res. 12: 137-146.