Human growth hormone-specific aptamer identification using improved oligonucleotide ligand evolution method

Protein Expression and Purification

Volumn 69, Issue 1, 2010, Pages 21-28

  Çalik, Pinar ^a   Balci, Oǧuz ^a   Özdamar, Tunçer H ^b  

^a MIDDLE EAST TECHNICAL UNIVERSITY   (Turkey)

^b ANKARA UNIVERSITY   (Turkey)

Author keywords

Aptamer; Elution; LETEG; Ligand; MALDI ToF; Protein; Purification; rhGH; Separation; ssDNA; Temperature gradient

Indexed keywords

APTAMER; BACTERIAL PROTEIN; HUMAN GROWTH HORMONE; IMMOBILIZED PROTEIN; LIGAND; RECOMBINANT PROTEIN;

ARTICLE; BACILLUS; CHEMISTRY; COMBINATORIAL CHEMISTRY; EXTRACELLULAR SPACE; FERMENTATION; HUMAN; ISOLATION AND PURIFICATION; KINETICS; MASS SPECTROMETRY; METABOLISM; METHODOLOGY; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; TEMPERATURE; TIME;

APTAMERS, NUCLEOTIDE; BACILLUS; BACTERIAL PROTEINS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; EXTRACELLULAR SPACE; FERMENTATION; HUMAN GROWTH HORMONE; HUMANS; HYDROGEN-ION CONCENTRATION; IMMOBILIZED PROTEINS; KINETICS; LIGANDS; RECOMBINANT PROTEINS; SELEX APTAMER TECHNIQUE; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; TEMPERATURE; TIME FACTORS;

BACILLUS SUBTILIS;

EID: 70350564295     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2009.05.015     Document Type: Article

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