In vitro and ex vivo models of extended reflux exposure demonstrate that weakly acidic mixed reflux heightens NF-kB-mediated gene expression

Diseases of the Esophagus

Volumn 24, Issue 5, 2011, Pages 360-370

  Cronin, J ^a   Alhamdani, A ^a,b   Griffiths, A P ^b   Baxter, J N ^b   Brown, T ^b   Jenkins, G J S ^a  

^a SWANSEA UNIVERSITY   (United Kingdom)

^b MORRISTON HOSPITAL   (United Kingdom)

Author keywords

Deoxycholic; Il 8; Nf kb; Oesophagus; Reflux

Indexed keywords

BILE ACID; DEOXYCHOLIC ACID; I KAPPA B; IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; INTERLEUKIN 8; CHOLAGOGUE; NFKB1 PROTEIN, HUMAN;

ARTICLE; CELL LINE; CONTROLLED STUDY; ESOPHAGUS BIOPSY; ESOPHAGUS PH; EX VIVO STUDY; GASTROESOPHAGEAL REFLUX; GENE EXPRESSION; GENE TARGETING; GENETIC TRANSCRIPTION; HUMAN; HUMAN CELL; HUMAN TISSUE; IN VITRO STUDY; PRIORITY JOURNAL; UPREGULATION; ADENOCARCINOMA; BARRETT ESOPHAGUS; DRUG EFFECTS; ESOPHAGUS TUMOR; GENETICS; METABOLISM; PH; SIGNAL TRANSDUCTION; TUMOR CELL LINE;

ADENOCARCINOMA; BARRETT ESOPHAGUS; CELL LINE, TUMOR; CHOLAGOGUES AND CHOLERETICS; DEOXYCHOLIC ACID; ESOPHAGEAL NEOPLASMS; GENE EXPRESSION; HUMANS; HYDROGEN-ION CONCENTRATION; I-KAPPA B PROTEINS; IN VITRO TECHNIQUES; INTERLEUKIN-8; NF-KAPPA B P50 SUBUNIT; SIGNAL TRANSDUCTION; UP-REGULATION;

EID: 79960575718     PISSN: 11208694     EISSN: 14422050     Source Type: Journal    
DOI: 10.1111/j.1442-2050.2010.01148.x     Document Type: Article

References (30)

1. Bremner C G, Mason R J. "Bile" in the oesophagus. Br J Surg 1993; 80: 1374-6.
2. Champion G, Richter J E etal. Duodenogastroesophageal reflux: relationship to pH and importance in Barrett's esophagus. Gastroenterology 1994; 107: 747-54.
3. Gotley D C, Morgan A P etal. Composition of gastro-oesophageal refluxate. Gut 1991; 32: 1093-9.
4. Fitzgerald R C, Omary M B, Triadafilopoulos G. Dynamic effects of acid on Barrett's esophagus. An ex vivo proliferation and differentiation model. J Clin Invest 1996; 98: 2120-8.
5. Jankowski J A, Harrison R F, Perry I, Balkwill F, Tselepis C. Barrett's metaplasia. Lancet 2000; 356: 2079-85.
6. Lagergren J, Bergstrom R etal. Symptomatic gastroesophageal reflux as a risk factor for esophageal adenocarcinoma. N Engl J Med 1999; 340: 825-31.
7. Locke G R 3rd, Talley N J etal. Prevalence and clinical spectrum of gastroesophageal reflux: a population-based study in Olmsted County, Minnesota. Gastroenterology 1997; 112: 1448-56.
8. Tselepis C, Morris C D, Wakelin D etal. Upregulation of the oncogene c-myc in Barrett's adenocarcinoma: induction of c-myc by acidified bile acid in vitro. Gut 2003; 52: 174-80.
9. Jenkins G J, Harries K etal. The bile acid deoxycholic acid (DCA) at neutral pH activates NF-kappaB and induces IL-8 expression in oesophageal cells in vitro. Carcinogenesis 2004; 25: 317-23.
10. Dvorak K, Payne C M, Chavarria M etal. Bile acids in combination with low pH induce oxidative stress and oxidative DNA damage: relevance to the pathogenesis of Barrett's oesophagus. Gut 2007; 56: 763-71.
11. Feagins L A, Zhang H Y, Zhang X etal. Mechanisms of oxidant production in esophageal squamous cell and Barrett's cell lines. Am J Physiol Gastrointest Liver Physio 2008; 294: G411-17.
12. Boonstra J J, van der Velden A W, Beerens E C W etal. Mistaken identity of widely used esophageal adenocarcinoma cell line Te-7. Cancer Res 2009; 67: 7996-8001.
13. Boonstra J J, van Marion R, Beer D G etal. Verification and unmasking of widely used human esophageal adenocarcinoma cell lines. J Natl Cancer Inst 2010; 102: 271-4.
14. Trowell O A. Cytocidal effects of radiation on organ cultures. Ann N Y Acad Sci 1961; 95: 849-65.
15. Browning J S, Trier T H. Organ culture of human small intestine. J Clin Invest 1969; 48: 85A.
16. Rafiee P, Nelson V M, Manley S etal. Effect of curcumin on acidic pH-induced expression of IL-6 and IL-8 in human esophageal epithelial cells (HET-1A): role of PKC, MAPKs, and NF-kappaB. Am J physiol 2009; 296: G388-398.
17. Triadafilopoulos G. Acid and bile reflux in Barrett's esophagus: a tale of two evils. Gastroenterology 2001; 121: 1502-5.
18. Ireland A P, Peters J H, Smyrk T C etal. Gastric juice protects against the development of esophageal adenocarcinoma in the rat. Ann Surg 1996; 224: 358-70.
19. Souza R F, Shewmake K, Pearson S etal. Acid increases proliferation via ERK and p38 MAPK-mediated increases in cyclooxygenase-2 in Barrett's adenocarcinoma cells. Am J Physiol Gastrointest Liver Physiol 2004; 287: G743-8.
20. Abdel-Latif M M, O'Riordan J etal. NF-kappaB activation in esophageal adenocarcinoma: relationship to Barrett's metaplasia, survival, and response to neoadjuvant chemoradiotherapy. Ann Surg 2004; 239: 491-500.
21. Hormi-Carver K, Zhang X, Zhang H Y etal. Unlike esophageal squamous cells, Barrett's epithelial cells resist apoptosis by activating the nuclear factor-kappaB pathway. Cancer Res 2009; 69: 672-7.
22. Konturek P C, Nikiforuk A, Kania J, Raithel M, Hahn E G, Muhldorfer S. Activation of NFkappaB represents the central event in the neoplastic progression associated with Barrett's esophagus: a possible link to the inflammation and overexpression of COX-2, PPARgamma and growth factors. Dig Dis Sci 2004; 49: 1075-83.
23. Rayet B, Gelinas C. Aberrant rel/nfkb genes and activity in human cancer. Oncogene 1999; 18: 6938-47.
24. Nehra D, Howell P etal. Toxic bile acids in gastro-oesophageal reflux disease: influence of gastric acidity. Gut 1999; 44: 598-602.
25. Jenkins G J, D'Souza F R etal. Deoxycholic acid at neutral and acid pH, is genotoxic to oesophageal cells through the induction of ROS: the potential role of anti-oxidants in Barrett's oesophagus. Carcinogenesis 2007; 28: 136-42.
26. Jolly A J, Wild C P, Hardie L J. Acid and bile salts induce DNA damage in human oesophageal cell lines. Mutagenesis 2004; 19: 319-24.
27. Hao Y, Sood S, Triadafilopoulos G etal. Gene expression changes associated with Barrett's esophagus and Barrett's-associated adenocarcinoma cell lines after acid or bile salt exposure. BMC Gastroenterol 2007; 7: 24.
28. Muhlbauer M, Allard B, Bosserhoff A K etal. Differential effects of deoxycholic acid and taurodeoxycholic acid on NF-kB signal transduction and IL8 gene expression in colonic epithelial cells. Am J Physiol Gastrointest Liver Physiol 2004; 286: G1000-1008.
29. Kauer W K, Peters J H etal. Mixed reflux of gastric and duodenal juices is more harmful to the esophagus than gastric juice alone. The need for surgical therapy re-emphasized. Ann Surg 1995; 222: 525-31; discussion 531-3.
30. Vaezi M F, Richter J E. Synergism of acid and duodenogastroesophageal reflux in complicated Barrett's esophagus. Surgery 1995; 117: 699-704.