E. coli and insect cell expression, automated purification and quantitative analysis

Methods in Molecular Biology

Volumn 498, Issue , 2009, Pages 143-156

  Chambers, Stephen P ^a   Fulghum, John R ^a   Austen, Douglas A ^a   Lu, Fan ^a   Swalley, Susanne E ^a  

^a VERTEX PHARMACEUTICALS   (United States)

Author keywords

Automated purification; E. coli and insect cell; Protein expression screen; Quantitative analysis

Indexed keywords

ESCHERICHIA COLI; HEXAPODA;

EID: 84934441135     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-59745-196-3_10     Document Type: Article

References (7)

1. Chambers, S. P., Austen, D. A., Fulghum, J. R., and Kim, W. M. (2004) High-throughput screening for soluble recombinant expressed kinases in Escherichia coli and insect cells. Protein Expr Purif. 36, 40-47.
2. Anderson, D., Harris, R., Polayes, D., Ciccarone, V., Donahue, R., Gerard, G., and Jessee, J. (1996) Rapid generation of recombinant baculoviruses and expression of foreign genes using Bac to Bac baculovirus expression system. Focus. 17, 53-58.
3. Luckow, V. A., Lee, S. C., Barry, G. F., and Olins, P.O. (1993) Efficient generation of infectious recombinant baculoviruses by site-specific transposon-mediated insertion of foreign genes into a baculovirus genome propagated in Escherichia coli. J Virol. 67, 4566-4579.
4. Swalley, S. E., Fulghum, J. R., and Chambers, S. P. (2006) Screening factors effecting a response in soluble protein expression: Formalized approach using design of experiments. Anal Biochem. 351, 122-127.
5. Bousse, L., Mouradian, S., Minalla, A., Yee, H., Williams, K., and Dubrow, R. (2001) Protein sizing on a microchip. Anal Chem. 73, 1207-1212.
6. Pijlman, G. P., van den Born, E., Martens, D. E., and Vlak, J. M. (2001) Autographa californica baculoviruses with large genomic deletions are rapidly generated in infected insect cells. Virology. 283, 132-138.
7. Donaldson, M.S., and Shuler, M.L. (1998) Effects of long-term passing of BTI-Tn5B1-4 insect cells on growth and recombinant protein production. Biotechnol Prog. 14, 543-547.